NR8383 AMs were cultured in Ham’s F12K medium supplemented with 15% fetal bovine serum (Excell Bio, Shanghai, China) in a humidified atmosphere with 5% CO2 at 37°C. Then, all of the cells were plated in a six-well plate for further experiments. The small interfering RNA (siRNA) duplexes for lncRNA4344 (NONRATT004344.2, sequence: 5ʹ-GCAGCAGAAGTCACTTATA-3ʹ) were commercially synthesized by RIBOBIO (RIBOBIO Co., Guangzhou, China). We used siRNA-lncRNA4344 and siRNA-scrambled to transfect NR8383 AMs at 70% confluence following Lipofectamine 2000 referencing the product instructions. After transfection for thirty-six hours, NR8383 AMs were exposed to LPS (1µg/ µL) (Sigma Chemical Co., USA) for 6 h. Total RNA was isolated and purified following the manufactory protocol. PCR analysis for lncRNAs and mRNAs was performed. The primer sequences for PCR are shown in Supplementary Table 1
Ham s f12k medium
Manufactured by ExCell Bio
Sourced in China
Ham's F12K medium is a cell culture medium formulation commonly used for the in vitro cultivation of a variety of cell types. It provides the necessary nutrients and growth factors to support the growth and maintenance of cells in a controlled laboratory environment. The composition of Ham's F12K medium is designed to optimize the growth and proliferation of the target cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by ExCell Bio (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Bulge loop mirna qrt pcr primer set, by RiboBio (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Propidium iodide, by MP Biomedicals (1 mentions)
Shikonin, by Selleck Chemicals (1 mentions)
Peg300, by Maclin Biochemical Technology (1 mentions)
Z vad fmk, by Selleck Chemicals (1 mentions)
Tween 80, by Maclin Biochemical Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by ExCell Bio (1 mentions)
Rpmi 1640, by ExCell Bio (1 mentions)
Anti human lc3b nb100 2220 antibody, by Novus Biologicals (1 mentions)
3 protocols using ham s f12k medium
1
Modulating lncRNA4344 in NR8383 AMs
2
Shikonin and Z-VAD-FMK Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Shikonin (>99.0%, #517-89-5) and Z-VAD-FMK (#S7023) were from Selleck Chemicals (Huston, TX, USA). Rapamycin (#D9542) was purchased from Sigma (St. Louis, MO, USA). PEG300 (#P815612) and Tween 80 (#T818928) were purchased from Macklin Biochemical Co (Shanghai, China) or Selleck Chemicals (#S6704 and S6702, respectively). Propidium iodide (PI, # ICN19545810) was purchased from MP Biomedicals (Solon, OH, USA). Cell lines were grown in DMEM (U2OS, PANC-1 and MDA-MB-231), RPMI-1640 (A549), or Ham’s F12K medium (LO2) with 10% FBS (ExCell bio, China) and 1% penicillin–streptomycin (Gibco/ThermoFisher, Franklin, MA, USA) at 37°C in 5% CO2 and 98% humidity.
3
Apoptosis Assay in Cancer Cell Lines
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