Pancreatic cancer cell lines PANC-1, BxPC-3, MiaPaCa-2, AsPC-1, Capan-1 and SUIT-2, as well as the epithelial cell line HPDE-E6E7, were purchased from ATCC and authenticated by the DKFZ Genomics Core Facility. All cells were regularly checked for mycoplasma contamination. PANC-1, BxPC-3 and Capan-1 were cultured in Iscove’s Modified Dulbecco’s Medium; MiaPaCa-2 and SUIT-2 were grown in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Waltham, MA, USA). AsPC-1 was maintained in RPMI-1640 medium (Thermo Fisher Scientific), HPDE-E6E7 cells in Keratinocyte-SFM medium (Thermo Fisher Scientific). Culture media were supplemented with 10% FBS, 1% penicillin and 1% streptomycin (Thermo Fisher Scientific). Cell growth was at 37 °C, 5% CO2 and 95% humidity.
Suit 2
Manufactured by American Type Culture Collection
Sourced in United States
The Suit-2 is a laboratory equipment designed for the safe handling and containment of biological materials. It provides a controlled environment for conducting various experiments and procedures involving potentially hazardous substances. The core function of the Suit-2 is to maintain a secure and isolated workspace, protecting both the user and the samples from potential contamination.
Automatically generated - may contain errors
Lab products found in correlation
Bxpc 3, by American Type Culture Collection (12 mentions)
Panc 1, by American Type Culture Collection (12 mentions)
Mia paca 2, by American Type Culture Collection (10 mentions)
Aspc 1, by American Type Culture Collection (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Capan 2, by American Type Culture Collection (6 mentions)
Capan 1, by American Type Culture Collection (5 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (4 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Hct116, by American Type Culture Collection (3 mentions)
Cfpac 1, by American Type Culture Collection (3 mentions)
Sw1990, by American Type Culture Collection (3 mentions)
Bxpc 3, by Merck Group (2 mentions)
Penicillin, by Fujifilm (2 mentions)
Streptomycin, by Fujifilm (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Cos 1, by American Type Culture Collection (2 mentions)
Hct15, by American Type Culture Collection (2 mentions)
Flp in t rex 293 cell, by Thermo Fisher Scientific (2 mentions)
Hek293, by American Type Culture Collection (2 mentions)
22 protocols using suit 2
1
Pancreatic Cancer Cell Lines: Cultivation and Authentication
2
Cultivation of Pancreatic Cancer Cell Lines
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PANC-1, a human pancreatic carcinoma of ductal origin were purchased from ATCC (Manassas, VA, USA) and SUIT-2, a human pancreatic cancer cell line established from liver metastasis from JCRB Cell Bank (Osaka, Japan). PANC-1 were cultivated in DMEM with 9% fetal bovine serum and 0.9% L-Glutamin, SUIT-2 in EMEM and 9.1% FBS, PaCa DD183 in DMEM, 13% FBS and 22% Keratinocyte-SFM, PANC-1SMAD4 (1−4) and PANC-1SMAD4 (2−6) in RPMI 1640, 9% FCS and 0.9% L-Glutamine. All cell lines were cultivated at 37°C and 5.0% CO2. For further passaging, trypsinization was performed using Trypsin-EDTA according to the manufacturer’s instructions.
3
Culturing Human Pancreatic Cancer Cells
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The human pancreatic cancer cell lines, BxPC-3 and SUIT-2, were purchased from the ATCC (Manassas, VA, United States). BxPC-3 cells were maintained in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, United States) supplemented with 10% fetal bovine serum (FBS) (Nichirei Biosciences, Tokyo, Japan), 100 U/mL penicillin-G sodium, and 100 mg/mL streptomycin sulfate (Invitrogen, Carlsbad, CA, United States) at 37 °C under a humidified atmosphere containing 5% CO2. SUIT-2 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (Wako, Osaka, Japan) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin.
4
Validating Cell Line Protocols
5
Culturing Human Pancreatic Cancer Cell Lines
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The human pancreatic cancer cell lines BxPC3 and SUIT2 were purchased from ATCC (Rockville, MD, USA) and from the JCRB Cell Bank (Osaka, Japan) respectively. Both the cell lines were maintained in RPMI‐1640 (Wako, Osaka, Japan) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 100 units/mL penicillin, and 100 μg/mL streptomycin and 0.25 μg/mL amphotericin B suspension (Wako) in a 5% CO2 incubator at 37°C.
6
Pancreatic Cancer Cell Lines Characterization
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Pancreatic cancer cell lines; CFPAC-1 (ATCC: CRL-1918), Suit-2, BxPC-3 (ATCC: CRL-1687) and Panc-1 (ATCC: CRL-1469) were obtained from ATCC (Rockville, MD) and maintained in a humidified incubator at 37 °C in RPMI-1640 containing 10% FBS, 2 mm l -glutamine, 2500 IU/mL penicillin and 5 μg/mL streptomycin (all from Sigma, Poole, UK). All cell lines were authenticated using short tandem repeat profiling against international reference standards.
7
Comprehensive PDAC Cell Line Cultivation
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Human PDAC cell lines (PANC1, MIA‐PaCa2, BxPC3, AsPC1, Capan‐2, SW1990, CFPAC1 and Suit2) were purchased from the ATCC. MIA‐PaCa2‐luc cells were purchased from JCRB Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan). MIA‐PaCa2, MIA‐PaCa2‐luc, BxPC3, AsPC1, Capan‐2, CFPAC1 and Suit2 cells were cultured in DMEM (Sigma‐Aldrich) supplemented with 10% FBS (Thermo Fisher Scientific). SW1990 and PANC1 cells were cultured in RPMI1640 medium (Sigma‐Aldrich) supplemented with 10% FBS.
8
Validating Cell Line Protocols
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The cell lines A549, COS1, HCT-15, HCT-116, HEK293, HeLa, SUIT2 and U2OS were from ATCC (Manassas, VA, USA) where each line was validated by short tandem repeat profiling. Flp-In T-REx293™ cells were from Thermo Fisher Scientific (Waltham, MA, USA) and validated by successful insertion of a gene of interest upon ectopic expression of Flp recombinase. KRAS4A null A549 and SUIT2 cells were generated by introducing an indel into exon 4A with CRISPR/Cas9, and HK1 and HK2 deficient HEK293 cells were generated by targeting the second and third exons of HK1 and HK2, respectively, with the same strategy. All cell lines were maintained in DMEM, except HCT-15 cells, which were grown in RPMI medium. All media were supplemented with 10% FBS and 1× penicillin/streptomycin. Transgene expression was induced in Flp-In T-REx293™ cells with 0.75 mg/mL doxycycline treatment for 24 h. Serum starving was performed with media containing 0.1%–1.0% FBS. All cell lines were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.
9
Cell Culture of Human Cancer Lines
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The human cancer cell lines A549, MCF7, MDA-MB-231, PANC-1, MIA Paca-2 SW1990, BxPC3, AsPC1, CFPAC1, Capan2, Suit2, COLO320HSR, SW480, HCT116, HepG2, and HeLa were purchased from ATCC or provided by the RIKEN BRC through the National Bio-Resource Project of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. The cell line M7609 was kindly provided by Dr. S. Machida (Hirosaki University, Hirosaki, Japan). All cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA) at 37 °C and under 5% CO2 condition.
10
PDAC Cell Line Culture Protocol
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PDAC (BxPC3, Suit2, Capan2, and MIA PaCa-2) cell lines were purchased from ATCC (Manassas, VA) and were grown according to standard procedures. All media were purchased from the Media Preparation Facility at Memorial Sloan Kettering Cancer Center (MSKCC). All cell lines were mycoplasma free and maintained at 37 °C in a humidified atmosphere at 5% CO2. Cell lines were authenticated at MSKCC integrated genomics operation core using short tandem repeat analysis, and used within passage number 15. Deidentified patient non-tumor and pancreatic cancer samples were obtained from David M. Rubenstein Center for Pancreatic Cancer Research following institutional review board (IRB) approval.
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