Acetone (SZBE082SV) of analytical grade was purchased from Sigma-Aldrich Co. (USA). Absolute ethanol (E/0650DF/15) was purchased from Fischer Chemicals (UK). Sterile deionized water was produced by a water purification system (Milli-Q Reagent Water System, MA, USA). Yeast extract and Bacto™ peptone were purchased from Lab M Ltd. (Lancashire, UK). Ammonium chloride, calcium chloride, cholesterol, ciprofloxacin hydrochloride, dextrose, dimethyl sulphoxide (DMSO, molecular biology grade), levamisole hydrochloride, magnesium sulfate, (±)-miconazole nitrate salt, rupintrivir, potassium dihydrogen phosphate, di–sodium hydrogen phosphate, sodium chloride, sodium hydroxide, and sucrose were all purchased from Sigma–Aldrich (MO, USA). MTS (3–[4,5 dimethylthiazol–2–yl]–5–[3–carboxymethyl–phenyl]–2–[4–sulphophenyl]–2H–tetrazolium, inner salt), was purchased from Promega, Leiden, The Netherlands.
Bacto peptone
Manufactured by Lab M
Sourced in United Kingdom
Bacto™ peptone is a complex nitrogen source derived from the enzymatic digestion of proteins. It provides a rich source of amino acids, peptides, and other nutrients required for the growth and cultivation of microorganisms in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Miconazole nitrate salt, by Merck Group (2 mentions)
Dextrose, by Merck Group (2 mentions)
Yeast extract, by Lab M (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
Ciprofloxacin hydrochloride, by Merck Group (1 mentions)
Levamisole hydrochloride, by Merck Group (1 mentions)
Rupintrivir, by Merck Group (1 mentions)
Magnesium sulfate, by Merck Group (1 mentions)
Dimethyl sulphoxide, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Ammonium chloride, by Merck Group (1 mentions)
3 protocols using bacto peptone
1
Analytical Grade Reagents for Microbial Assays
2
Cytotoxicity and Inflammatory Assays
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Sodium hydroxide (NaOH), Bradford reagent, glucose, disodium hydrogen phosphate (Na2HPO4), yeast extract, citric acid (C6H8O7.H2O), methanol, and acetone were purchased from Merck KGaA Germany. Bacto peptone was from Lab M (MC024, UK); Gentamicin was purchased from Biochrom, UK. Bovine serum albumin (BSA), Fetal bovine serum (FBS), L-glutamine, tween 20®, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl- tetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO), trypan blue, sulfuric acid (H2SO4), Phorbol myristate acetate (PMA), ethanol, lipopolysaccharide (Escherichia coli LPS25), fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) [A-9771] were from Sigma Chemicals, USA. A syringe filter (0.22 μm) was provided from Sartorius, France. RPMI 1640 medium, phosphate buffer saline (PBS), human TNF-α and human IL-6 ELISA kit, and Imject™ alum adjuvant were purchased from Thermo Fisher Scientific, USA., DAPI (6-diamidino-2-phenylindole) Fluoromount – G (SouthernBiotech, 0100–20).
3
Antimicrobial Susceptibility Testing Procedure
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Acetone, acetonitrile, methanol, hexane, and ethyl acetate (all of HPLC grade) were purchased from Sigma-Aldrich Co. (USA). Sterile deionized water was produced by a water purification system (Milli-Q Reagent Water System, MA, USA). Yeast extract and Bacto™ peptone were purchased from Lab M Ltd. (Lancashire, UK) (Panda et al., 2018 (link)). Dimethyl sulfoxide (DMSO, molecular biology grade), dextrose, amphotericin B, (±)-miconazole nitrate salt, Roswell Park Memorial Institute (RPMI) medium with L-glutamine without sodium bicarbonate, and 4-morpholinepropanesulfonic acid (MOPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Resazurin dye was purchased from Acros Organics (New Jersey, USA) (Kipanga and Luyten, 2017 (link)).
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