Western blot analysis was performed as previously described.24 (link) Three independent experiments in a certain condition were subjected to Western blot analysis. The CAV2 (ab3417), EGFR (ab52894), PI3K (ab40776), Akt (ab38449) and GAPDH (ab8245) antibody were purchased from Abcam Inc. The antibody was dilution at 1:1,000.
Akt ab38449
Manufactured by Abcam
Sourced in United Kingdom
Akt (ab38449) is a primary antibody that recognizes the protein kinase Akt. Akt is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes, including cell proliferation, differentiation, and survival. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and study the expression and localization of Akt in biological samples.
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Lab products found in correlation
Gapdh ab8245, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ecl chemiluminescence detection kit, by Beyotime (1 mentions)
Gsk3β ab32391, by Abcam (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Abcam (1 mentions)
Bca protein concentration detection kit, by Beyotime (1 mentions)
2 protocols using akt ab38449
1
Western Blot Analysis of Key Proteins
2
Protein Expression Profiling in Cell Lines
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An FBN1 immunohistochemistry kit was purchased from Shanghai Qi Ming Biotechnology Co., Ltd. Fetal bovine serum, Roswell Park Memorial Institute (RPMI)-1640 medium, and trypsin were all purchased from Gibco, USA. The DKK-8 kit was purchased from Shanghai Enzyme-linked Biology. An Annexin V-FITC/propidium iodide (PI) staining kit was purchased from Merck Sigma-Aldrich, USA. The PrimeScript™ RT reagent Kit (Perfect Real Time) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) were purchased from Beijing Baoriyi Material Technology Co., LTD. BCA protein concentration detection kit and ECL chemiluminescence detection kit were purchased from Shanghai Beyotime Biotechnology Co., Ltd. Rabbit polyclonal antibody FBN1 (ab53076), rabbit polyclonal antibody FBN1 (ab231094), rabbit polyclonal antibody AKT (ab38449), rabbit monoclonal antibody GSK3β (ab32391), mouse monoclonal antibody β-actin (ab8226), and rabbit anti-human horseradish peroxidaseconjugated IgG secondary antibody (ab6759) were purchased from Abcam company, UK.
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