G007 lk

MTT assays were performed to quantify the viability of the cells following treatment with G007-LK (MedChemExpress: Cat. No. HY-12438), a tankyrase inhibitor, and Olaparib (LC Laboratories, Cat. No. O-9201), a poly(ADP-ribose) polymerase inhibitor. H58 cells were seeded into 96-well plates at a concentration of 5000 cells per well and left to attach overnight at 37˚C and 5% CO₂. G007-LK was then added to the culture medium to a final concentration of 0, 0.1, 0.5, 1, 2, 4 μmol/L. Olaparib was then added to the culture medium to a final concentration of 0, 1, 5, 10, 20, 40 μmol/L. After 96 hours, MTT (Thermo Fisher Scientific, Cat. No. M6494) was added to the culture media, and the cells were incubated for 4h at 37 ˚C. The culture media was then removed and the formazan crystals in the cells were solubilized using dimethyl sulfoxide (Sigma-Aldrich, Cat. No. 472301), with plate agitation for 10 min. Absorbance at 490 nm was the measure using a Bio-Rad Xmark Microplate Spectrophotometry (Bio-Rad, Hercules, California, USA).