Anitbiotic antimycotic

Ribavirin (>98% purity) and MTT were purchased from Tokyo Chemical Industry (Tokyo, Japan) and Sigma-Aldrich (St. Louis, MO), respectively. Anitbiotic-antimycotic and minimum essential medium (MEM) were purchased from Invitrogen (Grand Island, NY). Fetal bovine serum (FBS) was supplied by PAA Laboratories (Etobicoke, Ontario, Canada). The protein molecular weight standards (Precision Plus Protein all blue standards) were purchased from Bio-Rad Life Sciences (Hercules, CA). RIPA buffer and 1% mammalian cell protease inhibitor cocktail were purchased from Sigma-Aldrich. The primary antibodies used in this study were as follows: anti-ICAM-1 antibody [rabbit polyclonal to intercellular adhesion molecule-1 (ICAM-1)], anti-low-density lipoprotein receptor (LDLR) antibody (rabbit polyclonal to LDLR), and anti-actin antibody (rabbit polyclonal to actin) purchased from Abcam (Cambridge, UK). The secondary antibody (horseradish peroxidase conjugated goat polyclonal to rabbit) was supplied by Abcam. All of the other chemicals and reagents used in this study were of analytical grade quality and available commercially.

Ascorbic acid, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (MTT), DPPH, gallic acid, quercetin, and sulforhodamine B (SRB) were purchased from Sigma-Aldrich (St. Louis, MO). 5,5-Dithiobis(2-nitrobenzoic acid) (DTNB), Folin-Ciocalteu’s phenol reagent, and acetylthiocholine iodide (ATChI) were purchased from Sigma-Aldrich. Commercially available antibiotic ciprofloxacin, anticancer agent cisplatin, and AChE inhibitors donepezil hydrochloride, huperzine A and tacrine were supplied by Sigma-Aldrich. Ribavirin was purchased from Tokyo Chemical Industry (Tokyo, Japan). Anitbiotic-antimycotic was purchased from Invitrogen (Grand Island, NY). Recombinant human BACE-1 and fluorogenic peptide substrate (FPS) Mca-SEVNLDAEFRK (Dnp) RR-NH₂ were purchased from R&D system (Minneapolis, MN). Brain Heart Infusion (BHI) broth and Eggerth-Gagnon (EG) agar were purchased from Becton, Dickinson and Company (Sparks, MD) and Eiken Chemical (Tokyo, Japan), respectively. Minimum essential medium (MEM), RPMI 1640 medium, and fetal bovine serum (FBS) were supplied by Life Technologies (Grand Island, NY). All of the other chemicals and reagents used in this study were of reagent-grade quality and available commercially.