The levels of total triglyceride (TG), total cholesterol (TC), high-density-lipoprotein cholesterol (HDLC), low-density-lipoprotein cholesterol (LDLC), and total bile acid (TBA) in serum were measured by analysis kits (A110-2, A111-2, A112-1, A113-1, and E003-2-1, Jiancheng Institute of Biotechnology, Nanjing, China). Non-esterified fatty acid (NEFA) was measured by an automatic biochemical analyzer (Olympus Au5400). The concentrations of very-low-density-lipoprotein-cholesterol (VLDL-C) and lysophosphatidylcholine (LPC) were measured by enzyme-linked immunosorbent assay kits (H249, Jiancheng, Nanjing and CEK621Ge, Cloud-clone corp, Wuhan, China, respectively) according to the instruction manual.
A110 2
Manufactured by Nanjing Jiancheng
Sourced in China
The A110-2 is a laboratory equipment that functions as a digital display platform. It provides precise numerical readouts for various measurements or data inputs.
Automatically generated - may contain errors
Lab products found in correlation
A111 2, by Nanjing Jiancheng (2 mentions)
A113 1, by Nanjing Jiancheng (1 mentions)
A112 1, by Nanjing Jiancheng (1 mentions)
Au5400, by Olympus (1 mentions)
E003 2 1, by Nanjing Jiancheng (1 mentions)
C009 2, by Nanjing Jiancheng (1 mentions)
A113 2, by Nanjing Jiancheng (1 mentions)
Ezrmi 13k, by Merck Group (1 mentions)
3 protocols using a110 2
1
Serum Lipid and Bile Acid Profiling
2
Biochemical Marker Quantification in Serum
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The levels of AST, ALT, TG, TC, LDL-C, HDL-C and glucose in the serum were detected with the Mindray BS-240VET Chemistry Analyzer (Mindray, Shenzhen, China). The hepatic TG was measured using a commercial kit (A110-2, Nanjing Jiancheng, Nanjing, China).
3
Metabolic Assessment in Fasted Mice
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Mice were fasted for 16 h before the tests. For the glucose tolerance tests (GTT), 2 g/kg glucose was injected intraperitoneally and plasma glucose levels were measured before the injection and at 15, 30, 60, 90, 120 and 150 min after the injection. For insulin tolerance tests (ITT), 0.75 U/kg insulin was given intraperitoneally and glucose levels in plasma were also determined before the injection and at 15, 30, 60, 90, 120 and 150 min after the injection. Fasting insulin levels was detected by an ELISA kit (EZRMI-13K, Millipore, Billerica, MA). Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as fasting insulin concentration (mU/L) × fasting glucose concentration (mmol/L)/ 22.5. Hepatic and plasma triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) were measured by enzymatic assay kits (A110-2, A111-2, A113-2, C009-2 Nanjing Jiancheng Bioengineering Institute, China). Serum levels of calcium and phosphate was determined by spectrophotometry (BC1655, BC0720, Beijing Solarbio Science & Technology Co. Ltd, China).
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