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1 1 diphenyl 2 picrylhydrazyl dpph 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox

Manufactured by Merck Group
Sourced in United States

1,1-diphenyl-2-picrylhydrazyl (DPPH) is a stable free radical compound commonly used as a reagent to evaluate antioxidant activity. 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) is a water-soluble vitamin E analog used as a standard in antioxidant capacity assays.

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3 protocols using 1 1 diphenyl 2 picrylhydrazyl dpph 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox

1

Comprehensive Evaluation of Vegetable and Woody Oils

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Vegetable oils, namely, peanut oil (PNO), soybean oil (SBO), corn oil (CO), flaxseed oil (FO), rapeseed oil (RSO), sunflower seed oil (SUSO), sesame seed oil (SESO), cotton seed oil (CSO), rice bran oil (RBO), pumpkin seed oil (PKSO), hemp seed oil (HSO), perilla seed oil (PLSO), peony seed oil (PNSO), safflower seed oil (SASO), grape seed oil (GSO), and seven woody oil, namely, walnut oil (WO), palm oil (PO), coconut oil (CNO), olive oil (OO), oil-tea seed oil (OSO), yellowhorn seed oil (YSO), sacha inchi oil (SIO) were obtained from local markets.
The Standards for fatty acid methyl esters, α-tocopherol, squalene, campesterol, stigmasterol, and β-sitosterol were from Sigma-Aldrich (St. Louis, MO, USA). The solvents used for the HPLC analysis are chromatography grade from Merck (Darmstadt, Germany). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), and 2′,7′-dichlorofluorescein diacetate (H2DCF-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals and solvents, like Folin-Ciocalteu, sodium carbonate, and potassium persulfate, were analytical grade purchased from Aladdin (Shanghai, China). Superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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2

Antioxidant Potential Evaluation

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All chemicals used in the experiments were analytical grade. Folin-Ciocalteu reagent, sodium carbonate, gallic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ascorbic acid, sulfuric acid, sodium phosphate, ammonium molybdate, oleuropein, and hydroxytyrosol were purchased from Sigma-Aldrich, Darmstadt, Germany.
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3

Antioxidant Potential of Inonotus obliquus from Tibet

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I. obliquus fruiting bodies were collected from Tibet’s Linzi Sera Mountain Region and their identity confirmed by Professor Cui Baokai of Beijing Forestry University and Professor Xu Asheng of the Institute of Plateau Ecology, Tibet Agricultural and Animal Husbandry Institute. Raw264.7 cell lines were purchased from tCell Resource Center of Shanghai Institute of Life Sciences, Chinese Academy of Sciences (Shanghai, China). DMEM medium and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St Louis, MO, USA). All other reagents were of analytical reagent grade and obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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