Cell isolates were washed in PBS, and reconsituted in MACS buffer with 0.1% bovine serum albumin (BSA) (Miltenyi, Gladbach, Germany). Cells were counted and the volume was adjusted for staining with nonhuman primate CD11b microbeads (Miltenyi). Forty million cells were reconstituted in 160 µl of MACS buffer and reacted with 80 µl of CD11b microbeads at 4°C for 15 min. After incubation, cells were washed with MACS buffer with 0.1% BSA, reconstituted into 500 µl of MACS buffer, and loaded onto MACS Separator LD columns. Both the negative flowthrough and the positve CD11b+ cells were collected, and cells were counted on Coulter Counter Z1. Isolates were used for downstream processing and analyzed for epitope expression using a fluorescence-activated cell sorter (FACS).
Nonhuman primate cd11b microbeads
Manufactured by Miltenyi Biotec
Sourced in Germany
Nonhuman primate CD11b microbeads are magnetic particles coated with antibodies that specifically bind to the CD11b antigen, which is expressed on the surface of myeloid cells in nonhuman primates. They can be used for the isolation or enrichment of these cell types from biological samples.
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Lab products found in correlation
2 protocols using nonhuman primate cd11b microbeads
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Isolation and Characterization of CD11b+ Cells
2
CD11b+ Cell Isolation and Characterization
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Cell isolates were washed in PBS, reconsituted in MACS buffer with 0.1% BSA (Miltenyi, Gladbach, Germany). Cells were counted and volume was adjusted for staining with nonhuman primate CD11b microbeads (Miltenyi, Gladbach, Germany). Forty million cells were reconstituted in 160 µl of MACS buffer and reacted with 80 µl of CD11b microbeads at 4°C for 15 minutes. After incubation, cells were washed with MACS buffer with 0.1% BSA, reconstituted into 500 µl of MACS buffer and loaded onto MACS Separator LD columns. Both the negative flow through and the positve CD11b cells were collected, cells were counted on Coulter Counter Z1. Isolates were used for downstream processing and analyzed for epitope expression using FACS.
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