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Deae cellulose

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DEAE cellulose is an ion exchange resin material used in chromatographic separation techniques. It is composed of cellulose with diethylaminoethyl functional groups attached, which allows for the reversible adsorption of charged molecules. The core function of DEAE cellulose is to facilitate the purification and isolation of biomolecules, such as proteins, enzymes, and nucleic acids, from complex mixtures.

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Lab products found in correlation

2 2 diphenyl 1 picrylhydrazyl (dpph), by Yuanye Bio-Technology (1 mentions) α glucosidase, by Yuanye Bio-Technology (1 mentions) Sepharose cl 6b, by GE Healthcare (1 mentions) Tocopherol, by Merck Group (1 mentions) Dialysis bag, by Yuanye Bio-Technology (1 mentions) Ethanol, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Acarbose, by Merck Group (1 mentions) P nitrophenyl α d glucopyranoside, by Merck Group (1 mentions) Sulfuric acid, by Nanjing Chemical Reagent (1 mentions) α glucosidase, by Merck Group (1 mentions) Sodium chloride (nacl), by Nanjing Chemical Reagent (1 mentions) Sodium hydroxide (naoh), by Nanjing Chemical Reagent (1 mentions) Anhydrous glucose, by Yuanye Bio-Technology (1 mentions) Methanol, by Nanjing Chemical Reagent (1 mentions) Petroleum ether, by Nanjing Chemical Reagent (1 mentions) Dimethyl sulfoxide (dmso), by Nanjing Chemical Reagent (1 mentions) Hplc grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions) Ab 8 macroporous resin, by Yuanye Bio-Technology (1 mentions)

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DEAE-cellulose 52 (12 citations)

4 protocols using deae cellulose

1

Characterization of Fungal Polysaccharides

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PE was purchased from Xi’an Chengxin Edible Fungus Planting Co., Ltd., Xi’an, China. DEAE cellulose, common dialysis bag (8–14 KDa), 2,2-diphenyl-1-picrylhydrazyl (DPPH, purity ≥ 98%), 4-nitrophenyl-d-pyridyl Glucosinolate (pNPG, purity ≥ 98%), and α-glucosidase(enzymatic activity ≥ 50 units/mg protein) were purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China. Glucose, xylose, arabinose, mannose, rhamnose, galactose, glucuronic acid, and standard were purchased from Hefei Bomei Biological Technology Co., Ltd., Hefei, China. Other chemicals used in the experiment were of analytical grade.
Gong P., Long H., Guo Y., Wang S., Chen F, & Chen X. (2022). Isolation, Structural Characterization, and Hypoglycemic Activities In Vitro of Polysaccharides from Pleurotus eryngii. Molecules, 27(20), 7140.
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2

Antioxidant Potential of Rana dybowskii

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Male ICR mice were provided by Changchun Yis Experimental Animal Technology Co., Ltd. and dried skin of Rana dybowskii Guenther, purchased from Taoyuan Forestry Farm, Shangying Forest Management Bureau, Shulan City, Jilin Province (127° 25′36.18″E; 44° 09′41.7″N; 338 m above sea level). Monosaccharide standards, including glucose (Glc), glucuronic acid (GlcA), N-acetylglucosamine (GlcNAc), galactose (Gal), galacturonic acid (GalA), N-acetylgalactosamine (GalNAc), mannose (Man), rhamnose (Rha), xylose (Xyl), arabinose (Ara), and fucose (Fuc), were purchased from Shanghai Yuanye Biotechnology Co. D-Galactose (D-Gal) was purchased from Sinopharm Chemical Reagent Co. Sepharose CL-6B (GE Healthcare), and DEAE-cellulose was obtained from Shanghai Yuanye Biotechnology Co. Assay kits for superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione peroxidase (GSH-Px) were provided by Jiancheng Institute of Biotechnology, Nanjing, China. All other chemicals and reagents were of analytical grade.
Li Y., Zhao X., Wang J., Yu Q., Ren J., Jiang Z, & Jiao L. (2024). Characterization and anti-aging activities of polysaccharide from Rana dybowskii Guenther. Frontiers in Pharmacology, 15, 1370631.
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3

Analytical-grade reagents protocol

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Analytical-grade reagents were purchased for this study: p-nitrophenyl-α-d-glucopyranoside (PNPG), tocopherol, hydroxybiphenyl, α-glucosidase (14.5 U/mg), acarbose, PBS (Sigma, Shanghai, China), ethanol, petroleum ether, methanol, DMSO, methanol (HPLC grade), 98% sulfuric acid, anthrone, sodium chloride, phosphate buffer, sodium carbonate, and sodium hydroxide (Nanjing Chemical Reagent Co., Ltd., China). AB-8 macroporous adsorption resin was purchased from Bengbu Liaoyuan New Materials Co., Ltd. Anhydrous glucose, DEAE cellulose, and dialysis bags were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Pectinase was purchased from Kandin Chemical Reagent Co., Ltd. (Guangzhou, China). DMEM was purchased from Thermo Fisher Scientific Instrument Co., Ltd. (Waltham, MA, USA).
Jiang P., Zhang Q., Zhao Y., Xiong J., Wang F., Zhang T, & Zhang C. (2019). Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z.. Molecules, 24(16), 2926.
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4

Cimicifuga heracleifolia Rhizome Analysis

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Dried Cimicifuga heracleifolia Kom. rhizomes (planted in Fushun, Liaoning China) were purchased in the herbal medicine market. AB-8 macroporous resin and DEAE-Cellulose were purchased from Yuanye Bio-Technology Company Limited (Shanghai, China). Bio-gel P-4 extra fine was purchased from Bio-Rad company (Hercules, CA, USA). HPLC grade acetonitrile (ACN) and 50% (w/w) sodium hydroxide (NaOH) solution for HPAEC were purchased from ThermoFisher Scientific (Waltham, MA, USA). Anhydrous sodium acetate (NaAc) for HPAEC study was obtained from J&K Scientific Ltd (Beijing, China). Saccharide standards, including mannose (Man), glucose (Glc), galactose (Gal), fructose (Fru), rhamnose (Rha), fucose (Fuc), xylose (Xyl), arabinose (Ara), galacturonic acid (GalA), glucuronic acid (GlcA), sucrose (Suc) and dextran with different molecular weights (i.e. Mw 1 kDa, 5 kDa, 25 kDa, 50 kDa, 80 kDa), were obtained from Sigma (St. Louis, MO, USA). Inulin standard (extracted from dahlia tubers) was purchased from Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All other chemicals and reagents were of analytical grade made in China.
Cui L., Wu J., Wang X., Yang X., Ye Z., Mayo K.H., Sun L, & Zhou Y. (2023). Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes. Food Chemistry: X, 18, 100706.
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