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Feg sirion xl

Manufactured by Thermo Fisher Scientific
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The FEG Sirion XL is a high-performance field emission gun scanning electron microscope (FEG-SEM) designed for advanced imaging and analysis applications. It features a high-brightness field emission gun source, providing improved spatial resolution and beam current characteristics.

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Solver pro, by NT-MDT (1 mentions)

2 protocols using feg sirion xl

1

Visualizing HEP/VEGF-SNC Formation on Bone Substitutes

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In order to analyze the formation of HEP/VEGF-SNCs on the bone substitutes, samples were fixed with 4% paraformaldehyde (PFA) for 10 min at 4 °C. After dehydration, the specimens were observed by mean of SEM (either with Hitachi TM1000 or FEG Sirion XL; FEI) in conventional high vacuum mode with a secondary electron detector. A commercial stand-alone AFM microscope Solver Pro (Nt-Mdt Inc., Moscow, Russia) was used to acquire AFM images. Tapping imaging mode was used, with NSG 10 cantilever with a typical resonance frequency of 105 kHz, and a spring constant of 2 N m−1. The image resolution was set to 512 × 512, with a scanning rate of 1 Hz. Images were analyzed using the open source software Gwyddion 2.24 [52 (link)].
Bornert F., Clauss F., Hua G., Idoux-Gillet Y., Keller L., Fernandez De Grado G., Offner D., Smaida R., Wagner Q., Fioretti F., Kuchler-Bopp S., Schulz G., Wenzel W., Gentile L., Risser L., Müller B., Huck O, & Benkirane-Jessel N. (2021). Mechanistic Illustration: How Newly-Formed Blood Vessels Stopped by the Mineral Blocks of Bone Substitutes Can Be Avoided by Using Innovative Combined Therapeutics. Biomedicines, 9(8), 952.
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2

Scaffold Morphology Analysis by SEM

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To analyse the morphology of the functionalized 3D PCL scaffolds, samples were fixed in paraformaldehyde 4% for 10 minutes at 4 °C, then dehydrated and observed with a scanning electron microscope (SEM Hitachi TM 1000 or FEG Sirion XL; FEI) in conventional mode (high vacuum) by collecting either secondary electrons or a combination of secondary and retrodiffused electrons.
Wagner Q., Offner D., Idoux-Gillet Y., Saleem I., Somavarapu S., Schwinté P., Benkirane-Jessel N, & Keller L. (2016). Advanced nanostructured medical device combining mesenchymal cells and VEGF nanoparticles for enhanced engineered tissue vascularization. Nanomedicine (London, England), 11(18).
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