Tgl flex

In KORA F4, total cholesterol (TC) was determined by cholesterol-esterase method (CHOL Flex, Dade-Behring, Germany), triglycerides (TG) and HDLC using the TGL Flex and AHDL Flex method (Dade-Behring), respectively, and LDLC was measured by a direct method (ALDL, Dade-Behring) [29] (link).
All participants were fasting for at least 8 hours. For the present analysis, all participants taking lipid-lowering drugs were excluded. The analysis dataset in KORA F4 is thus based on 2553 participants with available STR measurements and genotypes derived from genome-wide SNP-chips and imputation.
In SAPHIR, blood samples were collected after an overnight fasting period. A complete lipoprotein profile including fasting TC, TG, HDLC and LDLC was determined using routine laboratory procedures (Roche Diagnostics GmbH, Mannheim, Germany). For statistical analysis, all participants taking lipid-lowering drugs were excluded. The analysis dataset in the SAPHIR Study is therefore based on 1648 participants.

Four serum lipid measurements (in mg/dl) were collected using the Dimension RxL (Dade Behring); total cholesterol was determined by cholesterol-esterase method (CHOL Flex, Dade-Behring, CHOD-PAP method), HDL-C cholesterol by the AHDL Flex (Dade-Behring, CHOD-PAP method after selective release of HDL-C), LDL-C cholesterol by the ALDL Flex (Dade Behring, CHOD-PAP method after colourless usage of all non-LDL-cholesterol) and triglycerides (TG) by the TGL Flex (Dade Behring, enzymatic colorimetric test, GPO-PAP method).

Anthropometric measurements were taken after removing shoes, heavy clothing and belts. Body mass index (BMI) was calculated as weight [kg] divided by height² [m²]. Blood pressure measurements were taken at the right arm after a rest period of at least five minutes in a sitting position and repeated three times at an interval of three minutes. The mean of the second and third measurement was calculated. A fasting venous blood sample was obtained from all study participants while sitting. Triglycerides were measured with the GPO-PAP-method (TGL Flex, Dade Behring, Marburg, Germany). Plasma high-sensitivity C-reactive protein (hsCRP) concentrations were measured using a latex enhanced nephelometric assay run on a BN II analyser (Dade Behring, Marburg, Germany). HsCRP values higher than 10 mg/l were excluded from the analysis to include only participants with subclinical inflammation. Glycated hemoglobin (HbA1c) was measured by high-performance liquid chromatography with spectrophotometric detection (Diamat Analyzer; Bio-Rad, Munich, Germany) and a coefficient of variation of 1.5%. The insulin resistance score (HOMA-IR) was calculated as fasting plasma glucose (mmol/l) × fasting serum insulin (mlU/l) / 22.5.