Imr 32

Manufactured by CLS Cell Lines Service

IMR-32 is a neuroblastoma cell line derived from a human male patient. It is a widely used model for the study of neuroblastoma, a common type of cancer that develops in the sympathetic nervous system. The IMR-32 cell line can be used for various research applications, including the investigation of cancer biology, drug development, and cellular signaling pathways.

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Lab products found in correlation

Sh sy5y, by CLS Cell Lines Service (3 mentions) Sk n as, by American Type Culture Collection (2 mentions) Chla 90, by Thermo Fisher Scientific (1 mentions) Imr 32, by Thermo Fisher Scientific (1 mentions) Sk n fi, by Merck Group (1 mentions) U2os cells, by Merck Group (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Sk n be 2, by Leibniz Institute DSMZ (1 mentions) Ampflstr identifiler kit, by Thermo Fisher Scientific (1 mentions) Mycoalert, by Lonza (1 mentions) Sk n be2 cells, by Leibniz Institute DSMZ (1 mentions)

3 protocols using imr 32

Culturing human neuroblastoma cell lines

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U-2 OS cells (Sigma-Aldrich) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. NB cell lines SK-N-FI (Sigma-Aldrich), SK-N-AS (ATCC), SH-SY5Y (CLS Cell Lines Service), CHLA-90 (CCOG), IMR-32 (CLS Cell Lines Service) and SK-N-BE(2) (DSMZ) cells were cultured in specific media. SK-N-FI, SK-N-AS and SH-SY5Y cells were cultured in DMEM supplemented with 10% FBS, 0.1 mM Non-Essential Amino Acids (NEAA), and penicillin/streptomycin. CHLA-90 cells were cultured in DMEM supplemented with 20% FBS, 1× ITS (Gibco) and penicillin/streptomycin. IMR-32 cells were cultured in Minimum Essential Medium (MEM) supplemented with 10% FBS, 1 mM sodium pyruvate, 1× GlutaMAX (Gibco) and penicillin/streptomycin. SK-N-BE(2) cells were cultured in DMEM/F-12 media supplemented with 10% FBS, 1× GlutaMAX and penicillin/streptomycin. All cell lines were routinely tested for mycoplasma contamination and confirmed to be mycoplasma negative.

Vaid R., Thombare K., Mendez A., Burgos-Panadero R., Djos A., Jachimowicz D., Lundberg K.I., Bartenhagen C., Kumar N., Tümmler C., Sihlbom C., Fransson S., Johnsen J.I., Kogner P., Martinsson T., Fischer M, & Mondal T. (2024). METTL3 drives telomere targeting of TERRA lncRNA through m6A-dependent R-loop formation: a therapeutic target for ALT-positive neuroblastoma. Nucleic Acids Research, 52(5), 2648-2671.

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Neuroblastoma Cell Line Maintenance

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All NB cell lines were maintained at 37 C with 5% carbon dioxide. IMR-32 cell line was maintained in EMEM supplemented with 10% FBS, antibiotics, 1% glutamax, 1% sodium pyruvate, and 1% nonessential amino acids. SH-SY5Y (catalog no.: 300154; CLS Cell Lines Service GmbH), IMR-32 (catalog no.: 300148; CLS Cell Lines Service GmbH), SK-N-BE(2) (catalog no.: ACC 632; DSMZ-German Collection of Microorganisms and Cell Cultures GmbH); and SK-N-F1 (94092304; Merck). SK-N-AS (ATCC; CRL-2137), SK-N-DZ (ATCC; CRL-2149), NB69, Kelly (ECACC; HPA Culture Collections) and SHEP (CRL-2269; ATCC) were obtained from Prof. Tommy Martinsson, University of Gothenburg, and they were authenticated using AmpFLSTR Identifiler Kit (Thermo Fisher Scientific; ref. 30) . All cell lines were maintained in DMEM supplemented with 10% FBS and antibiotics. The cell lines were tested for Mycoplasma on regular intervals of 4 to 6 weeks using Mycoalert (Lonza LT07-418), and all the cell lines were used for five to seven passages upon thawing.

Mitra S., Muralidharan S.V., Di Marco M., Juvvuna P.K., Kosalai S.T., Reischl S., Jachimowicz D., Subhash S., Raimondi I., Kurian L., Huarte M., Kogner P., Fischer M., Johnsen J.I., Mondal T, & Kanduri C. (2021). Subcellular Distribution of p53 by the p53-Responsive lncRNA NBAT1 Determines Chemotherapeutic Response in Neuroblastoma. Cancer research, 81(6).

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Cell Line Cultivation Protocols

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SH-SY5Y, IMR-32, and Kelly cells were purchased from CLS Cell Lines Service. SK-N-BE2 cells were obtained from DSMZ, German Collection of Microorganisms and Cell Cultures GmbH. Cell lines used in this study were maintained at 37°C with 5% CO₂ and cultured in respective media as per manufacturer’s instructions.

Juvvuna P.K., Mondal T., Di Marco M., Kosalai S.T., Kanduri M, & Kanduri C. (2021). NBAT1/CASC15-003/USP36 control MYCN expression and its downstream pathway genes in neuroblastoma. Neuro-oncology Advances, 3(1), vdab056.

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