Trans 2 3 4 tert butylphenyl 2 methyl 2 propenylidene malononitrile dctb

Manufactured by Merck Group

Sourced in United States, China, Italy

Trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile (DCTB) is a chemical compound used as a matrix in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It is a crystalline solid that absorbs light in the ultraviolet and visible regions of the electromagnetic spectrum.

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5 protocols using trans 2 3 4 tert butylphenyl 2 methyl 2 propenylidene malononitrile dctb

Ganglioside Synthesis Inhibitor PDMP Protocol

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The GM1 probe Alexa Fluor 647 conjugated CTB was obtained from Thermo Fisher Scientific (C34778; Waltham, MA, USA). The ganglioside synthesis inhibitor PDMP was purchased from Cayman Chemical (62595; Ann Arbor, MI, USA). DDC (137030), 2,5-Dihydroxybenzoic acid (DHB; 149357), trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile (DCTB; 727881), cesium triiodide (483338), acetonitrile (271004), and trifluoroacetic acid (A2889) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A standard rodent chow diet CE-2 containing 0.1% wt/wt DDC was prepared by Japan Clea (Tokyo, Japan).

Su T., Qin X.Y., Dohmae N., Wei F., Furutani Y., Kojima S, & Yu W. (2021). Inhibition of Ganglioside Synthesis Suppressed Liver Cancer Cell Proliferation through Targeting Kinetochore Metaphase Signaling. Metabolites, 11(3), 167.

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Analytical Redox Reaction Protocols

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Analytical
grade chemicals, HCl (Tianjin Kaitong Chemical Co. Tianjin, China),
NaIO₄ (Tianjin Damao Chemical Reagent Company, Tianjin,
China), NaOH, 1,10-phen, NaN₃, ascorbic acid, thiourea,
H₂O₂ (Beijing Chemical Reagents Co. Beijing,
China), 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) (Tokyo
Kasei Kogyo Co. Ltd. Tokyo, Japan), nitrotetrazolium blue (NBT) (Nacalai
Tesque Inc. Tokyo, Japan), neocuproine, 5-nitro-1,10-phen, 3,4,7,8-tetramethyl-1,10-phen,
1,10-phen-5,6-dione, 4,7-phen (J & K Co. Ltd. Beijing, China),
1,10-phen-5-amine, 5,6-epoxy-5,6-dihydro-[1,10]phen, 1,7-phen,4,7-dihydroxy-1,10-phen,
5,6-dimethyl-1,10-phen, 2,2,6,6-tetramethyl-4-piperidine, tetradecahydro-4,7-phen, trans-2-[3-(4-tert-butylphenyl)-2-methyl-2-propenylidene]-malononitrile
(DCTB), and phenanthrene (Sigma-Aldrich) were purchased and used as
such. All solutions were prepared in distilled water.

Shah S.N., Shah A.H., Dou X., Khan M., Lin L, & Lin J.M. (2019). Radical-Triggered Chemiluminescence of Phenanthroline Derivatives: An Insight into Radical–Aromatic Interaction. ACS Omega, 4(12), 15004-15011.

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Comprehensive Mass Spectrometry Protocol

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Chloroform, acetonitrile (ACN),
methanol (MeOH), ammonium acetate, ethanol (EtOH), dichloromethane
(DCM), formic acid, and isopropanol (IPA) were from Merck (Darmstadt,
Germany). Trypsin (0.25%), Dulbecco’s modified eagle medium
(DMEM), dimethyl sulfoxide (DMSO), 4-chloro-phenylalanine (4-Cl-Phe),
fetal bovine serum (FBS), collagen I, and penicillin–streptomycin
(100 U/mL) were from Thermo Fisher (Cambridge, MA, U.S.A.). trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile
(DCTB), 2,5-dihydroxybenzoic acid (DHB), orlistat, and 9-acridinamine
(9AA) were from Sigma-Aldrich (St. Louis, MO, U.S.A.). Phosphatidylcholine(19:0/19:0)
(PC(19:0/19:0)), triglyceride(15:0/15:0/15:0) (TG(15:0/15:0/15:0)),
and sphingomyelin(d18:1/12:0) (SM(d18:1/12:0)) were obtained from
Avanti Polar Lipids (Alabaster, AL, U.S.A.). TCS glucuronide (TCSG,
95%) and TCS sulfate (TCSS, 95%) were purchased from Santa Cruz Biotechnology
(Dallas, TX, U.S.A). TCS (99%) was brought from Alfa Aesar (Haverhill,
MA, U.S.A). ¹³C₁₂-TCS was purchased from Cambridge
Isotope Laboratories (Andover, MA, U.S.A).

Chen J., Xie P., Wu P., Lin Z., He Y, & Cai Z. (2023). Spatial Metabolomics and Lipidomics Reveal the Mechanisms of the Enhanced Growth of Breast Cancer Cell Spheroids Exposed to Triclosan. Environmental Science & Technology, 57(29), 10542-10553.

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Polymer Characterization with PLA Standards

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The PLA standards used in this
investigation were purchased from PSS Polymer Standards Service GmbH,
now part of Agilent Technologies (Mainz, Germany). The molar mass
information of all the standards based on universal calibration and
the putative stereochemistry defined by the supplier is provided in Table 1. The eluents, amylene-stabilized
chloroform (TCM) and hexane (HEX) were purchased from Sigma-Aldrich.
The isopropanol, tetrahydrofuran (THF), and ethanol-stabilized TCM
were obtained from Fisher Scientific, TH. GEYER, and VWR International,
respectively, and were all HPLC grade and were used as received.
For NMR analysis, deuterated chloroform (Deutero GmbH) was used, while
the trans-2-[3-(4-tert-butylphenyl)-2-methyl-2-propenylidene]
malononitrile (DCTB) and sodium trifluoroacetate (NaTFA) used as the
matrix and ionizing agent in MALDI experiments, respectively, were
purchased from Sigma-Aldrich. MS calibration standards PMMA fleXstandard
were obtained from Bruker Daltonics and SpheriCal Neat Protein Medium
from Polymer Factory.

Eselem Bungu P.S., Luetzow K., Lettau O., Schulz M., Neffe A.T, & Pasch H. (2024). Stereochemical Heterogeneity Analysis of Polylactides by Multidimensional Liquid Chromatography. Analytical Chemistry, 96(11), 4716-4725.

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Synthesis of Alkyl Halides and Polymers

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1-Methylimidazole (purity 99%), 1-bromononane (purity 98%), 1-bromodecane (purity 98%), 1-bromododecane (purity 97%), 1-bromotetradecane (purity 97%), 1-bromohexadecane (purity 97%) 1-bromooctadecane (purity ≥ 97%), 1-bromoeicosane (purity ≥ 97%), 1,3-dimethyl-5-sulfoisophthalate sodium salt (purity 98%), dichloromethane (DCM) (purity ≥ 99.9%), tetrafluoroborate sodium salt (purity 98%), anhydrous sodium sulphate (purity 99%), silver nitrate (purity ≥ 99%), ethyl acetate (purity ≥ 99.5%), tetrahydrofuran (THF) (purity 99.9%), dimethyl sulfoxide-d₆ (DMSO-d₆) (99.9 atom % D), trans-2-[3-(4-tert-butylphenyl)-2-methyl-2-propenylidene] malononitrile (DCTB) (purity ≥ 98%) were purchased from Sigma-Aldrich (Milan, Italy) and used as received. The PVC used in this study was plasticised with tris (2-ethylhexyl) trimellitate (TOTM). It was furnished by Consorzio Proplast (Alessandria, Italy) and had a K-value of 65.0, hardness (shore A) of 87.5, and specific gravity of 1.242.

Novello E., Scalzo G., D’Agata G., Raucci M.G., Ambrosio L., Soriente A., Tomasello B., Restuccia C., Parafati L., Consoli G.M., Ferreri L., Rescifina A., Zagni C, & Zampino D.C. (2024). Synthesis, Characterisation, and In Vitro Evaluation of Biocompatibility, Antibacterial and Antitumor Activity of Imidazolium Ionic Liquids. Pharmaceutics, 16(5), 642.

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