Pcs 200 040

Fish collagen type I (FCOL) was purchased from Creative Enzymes Inc. (Shirley, NY, USA), hyaluronic acid (HA) was purchased from Wisapple Biotech Co, Ltd. (Beijing, China), Pronatal^® LF 10/60 SA (SA 10/60) with G/M % ratios of 70/30 was kindly gifted by IMCD UK Limited, (Surrey, UK), sodium alginate (SA) with G/M % ratio 39/61, Gelatin (GEL) and D-mannitol (D-mann) were purchased from Sigma-Aldrich (Gillingham, UK)., bovine serum albumin (BSA), and calcium chloride were obtained from Acros Organics (Branchburg, NJ, USA) while sodium chloride (NaCl) was purchased from Fisher Scientific, (Loughborough, UK). Adult human primary epidermal keratinocytes [PCS-200-011, ATCC], human dermal fibroblasts [PCS-200-011, ATCC], dermal cell basal medium [PCS-200-030, ATCC], keratinocytes growth kit [PCS-200-040, ATCC], ATCC and Dulbecco’s Modified Eagle’s Medium (DMEM) [PCS-200-030, ATCC] were purchased from LGC standards (Middlesex, UK). Methyl thiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue stain were obtained from Thermo Fisher Scientific (Paisley, UK), fetal bovine serum was purchased from Sigma Aldrich, (Dorset, UK).

Human OSCC cell lines derived from oral cancer (SAS, HSC-2, HSC-3, Ca9-22, OSC-19, OSC-20, SAT, and KON) were purchased from the National Institute of Biomedical Innovation (Osaka, Japan). The HOC-313⁵⁴ (link) and TSU⁵⁵ (link) cell lines were kindly provided by Professor Kawashiri (Kanazawa University). HNOK cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA; PCS-200-014). SAS-R and HSC-2-R, which were established from SAS and HSC-2 cells, were used as the CRR cell lines. The CRR cell lines were produced by exposing cells to gradually increasing X-ray doses.⁵ (link) The OSCC cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM; D6429; Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich) at 37°C and 5% CO₂. HNOK cells were cultured in dermal cell basal medium (PCS-200-030; ATCC) supplemented with the Keratinocyte Growth Kit (PCS-200-040; ATCC). CRR cells continued to proliferate under a daily IR dose of 2 Gy for >30 days in vitro.