Rheumatoid factor was determined using the rate nephelometric test as per the manufacturer’s instructions (Beckman ICS II, Beckman Coulter). ACPA titers were determined by the EliA test with UniCAP (Phadia Laboratory Systems, Uppsala, Sweden).
Beckman ics 2
Manufactured by Beckman Coulter
The Beckman ICS II is an ion chromatography system designed for the analysis of various ions in aqueous samples. It is capable of performing separations and quantitative determinations of cations, anions, and weak acids or bases. The system utilizes suppressed conductivity detection for sensitive and selective measurements.
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Lab products found in correlation
2 protocols using beckman ics 2
1
Quantifying Rheumatoid Factor and ACPA
2
Quantification of Immune Biomarkers in Rheumatoid Arthritis
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RF titration was determined by nephelometry (Beckman ICS II, Beckman Coulter). ACPA titre was determined by EliA test using UniCAP (Phadia Laboratory Systems, Uppasala, Sweden). Plasma was tested for IL-17, IL-22, VEGF, sCD40L (Peprotech, London, UK), soluble P-Selectin (sCD62P), soluble CD162 (sCD162) (R&D Systems, Minneapolis, MN), Lipopolysaccharide binding protein (LBP) (Hycult Biotech, Uden, The Netherlands), IL-10, IL-6 and soluble IL-6 receptor (sIL-6R) (ImmunoTools), using specific ELISAs according to the manufacturer’s instructions. All cytokines were quantified with standard curves provided by manufacturer. The detection limits were: 31.2 pg/mL for IL-17, 15.6 pg/mL for IL-22, 31.2 pg/mL for VEGF, 15.6 pg/mL for sCD40L, 125 pg/mL for sCD62P, 31.3 pg/mL for sCD162, 4.4 ng/mL for LBP, 16 pg/mL for IL-10, 3 pg/mL for IL-6 and 230 pg/mL for sIL-6R.
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