IF and CP were purchased from Shionogi and Co., Ltd. (Osaka, Japan), and BD was obtained from SymBio Pharmaceuticals Ltd. (Tokyo, Japan). Methanol, ethanol, 1-propanol, and acetone were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). The chemical structures of IF, CP and BD are shown in Fig. 1.
Ethanol
Manufactured by Kanto Chemical
Sourced in Japan
Ethanol is a clear, colorless liquid chemical compound commonly used as a solvent, disinfectant, and fuel additive. It has the chemical formula C2H6O.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Kanto Chemical (10 mentions)
Acetone, by Kanto Chemical (9 mentions)
Ethyl acetate, by Kanto Chemical (5 mentions)
Hexane, by Kanto Chemical (4 mentions)
N n dimethylformamide (dmf), by Kanto Chemical (3 mentions)
Tetrahydrofuran, by Kanto Chemical (3 mentions)
Chloroform, by Kanto Chemical (3 mentions)
Potassium hydroxide, by Kanto Chemical (3 mentions)
Toluene, by Kanto Chemical (3 mentions)
1 propanol, by Kanto Chemical (2 mentions)
Sodium hydroxide (naoh), by Kanto Chemical (2 mentions)
Hydrogen peroxide, by Fujifilm (2 mentions)
Toluene, by Fujifilm (2 mentions)
Hydrochloric acid (hcl), by Fujifilm (2 mentions)
Pyridine, by Kanto Chemical (2 mentions)
Nitric acid, by Kanto Chemical (2 mentions)
Lithium chloride (licl), by Fujifilm (2 mentions)
2 propanol, by Kanto Chemical (2 mentions)
Dichloromethane, by Kanto Chemical (2 mentions)
Hydrochloric acid (hcl), by Kanto Chemical (2 mentions)
39 protocols using ethanol
1
Synthesis and Characterization of Novel Compounds
2
Synthesis of Silver Nanowire Ink
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Example 4
174 g of the methanol dispersion liquid of silver nanowire 2 (silver concentration: 0.2% by mass, dispersion medium: methanol, wire average diameter: 36 nm, average length: 20 μm) produced as above, was weighed in a 1000 ml eggplant flask. 3.1 g of 10% by mass PNVA aqueous solution (manufactured by Showa Denko K. K.), 40.9 g of propylene glycol (manufactured by Wako Pure Chemical Industries, Ltd.), and 112.3 g of PGME (manufactured by Tokyo Chemical Industry Co., Ltd.) were added thereto, and dispersed well. Thereafter, methanol was distilled away, by using an evaporator. Then, 63.2 g of pure water, 300 g of ethanol (manufactured by Kanto Chemical Co., Inc.), and 49.3 g of methanol (manufactured by Junsei Chemical Co., Ltd.) were added and stirred by using planetary centrifugal vacuum mixer Awatori Rentaro (registered trademark) ARV-310, manufactured by Thinky Corporation, to obtain silver nanowire ink 4 having a mass ratio between the binder resin (PNVA) and the silver nanowire (binder resin/silver nanowire) of 0.87.
3
Synthesis and Characterization of Polymers
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Acetone, hexane, tetrahydrofuran (THF), methanol, chloroform, ethyl acetate (EtOAc), ethanol, acetonitrile, acryloyl chloride, sodium hydroxide, potassium hydroxide, N, N-dimethylformamide (DMF), anhydrous magnesium sulfate, and diethyl ether were purchased from Kanto Chemical. Copper (I) chloride (CuCl), lithium bromide (LiBr), ethylamine, and DIBOD were purchased from the Tokyo Chemical Industry. Sodium azide and dibenzocyclooctyne-amine (DBCO-amine) were purchased from Sigma-Aldrich. N, N-dimethylformamide (DMF) for preparing the GPC/SEC’s eluent and hydrochloric acid (12 N) were purchased from FUJIFILM Wako Pure Chemical Industry. Pentaerythritol tetra (2-chloropropionate) (PETCP) was commercially obtained as a custom-made product from the Tokyo Chemical Industry and used as an initiator. Ultrapure water (specific resistance value: 18.2 MΩ cm) obtained by purifying tap water with a Direct-Q 3UV manufactured by Millipore was used. In addition, N-isopropyl acrylamide (NIPA) and tris (2-dimethylamino ethyl) amine (Me6TREN) were kindly provided by Kohjin and Mitsubishi Chemical, respectively. NIPA was purified by recrystallization using a mixture of hexane and Acetone. Me6TREN (Mitsubishi Chemical) and DMF (Kanto Chemical) for synthesis were purified by vacuum distillation. Furthermore, other chemicals were used without purification.
4
Synthesis of Iridium Particle Complex
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Example 47
[HPSN(C2H4OC2H4OCH3)3Br]Ir Particle Complex
Into a two-neck flask (100 mL) equipped with a cooling tube, HPSNBr(C2H4OC2H4OCH3)3 (190 mg) and IrCl3.3H2O [manufactured by Furuya Metal Co., Ltd.] (84 mg) were charged and thereto, further ethanol [manufactured by Kanto Chemical Co., Inc.] (15 mL) and distilled water (15 mL) were added, followed by purging the inside of the flask with nitrogen. Under heating-reflux (80° C.), the resultant reaction mixture was stirred for 12 hours. After the reaction, the solution was filtered with a membrane filter and from the resultant filtrate, the solvent was distilled off with a rotary evaporator. The resultant residue was re-dissolved in chloroform [manufactured by Kanto Chemical Co., Inc.] (10 mL) and the resultant solution was dropped into hexane [manufactured by Kanto Chemical Co., Inc.] (30 mL) to subject the solution to re-precipitation purification. An ocher solid (192 mg) of an [HPSNBr(C2H4OC2H4OCH3)3]Ir particle complex was obtained. The Ir content was measured by a DTA-TG measurement and found to be 24.8% by mass. The result of the DTA-TG measurement is shown in
5
Fabrication and Application of Microfluidic Devices
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All chemical reagents used in this work were of analytical grade and water was purified by a Milli-Q® Reference (Nippon Millipore, Tokyo, Japan) system. ENG hard resin used for fabrication of the on-chip injector and a template for microfluidic device was obtained from Kudo 3D Inc. (CA, U.S.A.). Polydimethylsiloxane (PDMS) prepolymer and curing agent were purchased from Dow Corning Toray Co., Ltd. (Tokyo, Japan). Isopropyl alcohol (IPA), hydrochloric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, ethanol and acetone were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). Tris(hydroxymethyl)aminomethane (Tris) was obtained from MP Biomedicals, Inc. (CA, U.S.A.). Food dyes (yellow and blue) were purchased from Kyoritsu Foods Co., Ltd. (Tokyo, Japan). Resorufin sodium salt and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Co. LLC (MO, U.S.A.). Amplex Red was obtained from Thermo Fisher Scientific, Inc. (MA, USA). Hydrogen peroxide and horseradish peroxidase (HRP) were purchased from FUJIFILM Wako Pure Chemical Corp. (Tokyo, Japan).
6
Quantification of AFP and E2 in Mouse and Bovine Sera
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Mouse AFP in the P0 mouse serum and bovine AFP levels in FBS used in this study were measured in duplicate using enzyme-linked immunosorbent assay kits (R&D Systems, for mouse; Cloud-Clone, for cow) according to the manufacturer's instructions. For E2 measurement in FBS, ethyl acetate was added to FBS or standard E2 (Kanto Chemical) solution dissolved in methanol (Kanto Chemical), and then mixed and centrifuged at 16,000 g for 3 min at 4°C. This step was repeated three times. These supernatants containing E2 were combined and dried under a nitrogen stream. The solid was reconstituted in ethanol (Kanto Chemical) and subjected to liquid chromatography-tandem mass spectrometry (Nexera UHPLC; API 4000, AB SCIEX).
7
Synthesis of Quantum Dots from Metallic Precursors
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Zinc(II) acetate dihydrate
(99.999%), copper(I) iodide (99.999%), and indium(III) acetate (99.99%)
were purchased from Sigma–Aldrich. OA (>85.0%), DDT (>95.0%),
1-octadecene (ODE; > 90.0%), and MPA (>98.0%) were purchased
from
Tokyo Chemical Industry. MPACH (>90.0%) was purchased from FUJIFILM
Wako Pure Chemical. Toluene (>99.5%), ethanol (>99.5%), chloroform
(99.0%), and hexane (96.0%) were purchased from Kanto Chemical. The
used celloxide, an alicyclic epoxy compound, was celloxide 8000 manufactured
by TOKYO OHKA KOGYO Co., Ltd. The UV-curable resin composed of celloxide
8000 and a photoacid generator at 2 wt % was also provided by the
same company. Toluene, ethanol, chloroform, and hexane were dehydrated
over molecular sieves (3A 1/8, FUJIFILM Wako Pure Chemical) prior
to use.
(99.999%), copper(I) iodide (99.999%), and indium(III) acetate (99.99%)
were purchased from Sigma–Aldrich. OA (>85.0%), DDT (>95.0%),
1-octadecene (ODE; > 90.0%), and MPA (>98.0%) were purchased
from
Tokyo Chemical Industry. MPACH (>90.0%) was purchased from FUJIFILM
Wako Pure Chemical. Toluene (>99.5%), ethanol (>99.5%), chloroform
(99.0%), and hexane (96.0%) were purchased from Kanto Chemical. The
used celloxide, an alicyclic epoxy compound, was celloxide 8000 manufactured
by TOKYO OHKA KOGYO Co., Ltd. The UV-curable resin composed of celloxide
8000 and a photoacid generator at 2 wt % was also provided by the
same company. Toluene, ethanol, chloroform, and hexane were dehydrated
over molecular sieves (3A 1/8, FUJIFILM Wako Pure Chemical) prior
to use.
8
Preparation of Functionalized Porous α-Alumina
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The porous α-alumina porous support (o.d. of 1.9–2.3 mm, average pore diameter of about 150 nm) was supplied by AIST (Japan). Tetraethyl orthosilicate (TEOS; >95.0%), HCl (35%), acryloyl chloride (>98.0%), acrylic acid (>98.0%), ammonium peroxodisulfate (APS; >98.0%), toluene (>95%), tetrahydrofuran (THF; >99.5%), acetic acid (>99.7%), L-lactic acid (85.0%–92.0%), phenol (>99.0%), 0.2 mol/L HCl, and 0.2 mol/L sodium hydroxide aqueous solution were purchased from FUJIFILM Wako Pure Chemical Corp., Osaka, Japan. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (>95.0%), ethanol (>99.5%), methanol (>99.8%), sodium tetraborate decahydrate (99.5–101.0%), disodium hydrogen phosphate dodecahydrate (>99.0%), and sodium dihydrogen phosphate dihydrate (99.0–102.0%) were purchased from Kanto Chemical Co., Ltd., Tokyo, Japan. 3-Aminopropyltrimethoxysilane (APTMS; >96.0%) was purchased from Tokyo Chemical Industry Co., Ltd., Tokyo, Japan. Sodium acetate (>98.0%) was purchased from Nacalai Tesque Inc., Kyoto, Japan. All chemicals were used without purification. Water was obtained from a water purification system (WG-204, Yamato Co., Tokyo, Japan).
9
Detailed SEM Sample Preparation
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The substratum was washed three times with D-PBS and immersed in 4% PFA for 1 day at 4°C. The cells were then washed three times with D-PBS, immersed in a 2.5% glutaraldehyde/D-PBS solution, and stored overnight at 4°C for pre-fixation. After washing with D-PBS, the cells were post-fixed with a 1% osmium oxide (Wako)/D-PBS solution and left at room temperature for 1 h. After osmium oxide treatment, the cells were washed with pure water and dehydrated by replacing the ethanol solution with increasing concentrations of ethanol (Kanto Chemical, Tokyo, Japan). The samples were then replaced with t-butyl alcohol (Wako Pure Chemical Industries) and lyophilized at −50°C. The samples were observed using SEM.
10
Synthesis of Aspartame Precursor
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l -Aspartyl-l -phenylalanine methyl ester was supplied by Ajinomoto Co., Inc. Triphenyl phosphite (P(OPh)3), l -phenylalanine (Phe), succinic acid, ethylene diamine and p-phenylenediamine were purchased from Tokyo Chemical Industry Co., Ltd. 5% Palladium on activated carbon (Pd/C) and N-methylpyrrolidone (NMP) were purchased from FUJIFILM Wako Pure Chemical Corporation. Dimethyl sulfoxide (DMSO), sulfuric acid, nitric acid, pyridine, ethanol, tetrahydrofuran, ethyl acetate, acetone and toluene were purchased from Kanto Chemical Co., Inc. All the chemicals were directly used as purchased.
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