Cd1b apc

Manufactured by BioLegend

CD1b-APC is a fluorochrome-conjugated antibody that specifically binds to the CD1b antigen. CD1b is a member of the CD1 family of antigen-presenting molecules that present lipid and glycolipid antigens to T cells. The APC (Allophycocyanin) fluorochrome allows for the detection and analysis of CD1b-expressing cells using flow cytometry.

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Lab products found in correlation

Cd80 fitc, by BD (1 mentions) Recombinant il32γ, by R&D Systems (1 mentions) Cd86 pe cy7, by BD (1 mentions) Cd14 apc cy7, by BD (1 mentions) Interleukin 4 (il 4), by CellGenix (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Hla dr percp cy5, by BD (1 mentions) Mouse igg1 fitc, by Beckman Coulter (1 mentions) Gm csf, by Sanofi (1 mentions) Cd16 32 antibody, by BioLegend (1 mentions) Cd40 pe, by BioLegend (1 mentions) Cd86 percp cy5, by BioLegend (1 mentions) Cd80 pe cy7, by BioLegend (1 mentions) Hla dr alexafluor700, by BioLegend (1 mentions) Kaluza flow cytometry analysis software, by Beckman Coulter (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Ccr7 pe, by BioLegend (1 mentions) Cd31 brilliant violet 421, by BioLegend (1 mentions) Plzf pe, by Thermo Fisher Scientific (1 mentions) Eomes fitc, by Thermo Fisher Scientific (1 mentions)

3 protocols using cd1b apc

Monocyte-to-Dendritic Cell Differentiation Assay

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PBMCs were collected using a ficoll gradient (Ficoll-Paque Plus, GE Healthcare) and monocytes were isolated by a CD14 positive selection (Miltenyi, Bergisch Gladbach, Germany). Monocytes were split into five experimental groups: (1) Negative Control (no cytokines), (2) GM–CSF (Sanofi) + IL-4 (Cell Genix) at 1000 U/ml, 3) Recombinant IL32α (R&D Systems) at 100 ng/ml, 4) Recombinant IL32β (R&D Systems) at 100 ng/ml, 5) Recombinant IL32γ (R&D Systems) at 100 ng/ml, and cultured using Cell Genix Media to yield immature DCs at day 5. Immature DC were harvested and surface stained for flow cytometry analysis. Cell surface markers were observed on the double positive, HLA-DR and CD86 population of cells. Antibodies used included CD80 FITC (BD, Clone L307.4), Mouse IgG1 FITC (Beckman Coulter PN IM0639U), CD86 Pe-Cy7 (BD, Clone FUN-1), HLA-DR PerCpCy5.5 (BD, Clone G46-4), CD1B APC (BioLegend, Clone SN13), CD14 APC-Cy 7 (BD, Clone MφP9), CD68 BV 711 (BD, Clone Y1/82A), Mouse IgG2B BV 711 (BD, Clone 27-35), and Zombie Aqua Viability Dye BV 510 (BioLegend).

Paz H., Tsoi J., Kalbasi A., Grasso C.S., McBride W.H., Schaue D., Butterfield L.H., Maurer D.M., Ribas A., Graeber T.G, & Economou J.S. (2019). Interleukin 32 expression in human melanoma. Journal of Translational Medicine, 17, 113.

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Phenotypic Analysis of Human Macrophages

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Collected macrophages were blocked using CD16/32 antibody (BioLegend, San Diego, CA) on ice for 15 min. The macrophages were then stained with anti-human HLA-A,B,C-FITC, HLA-DR-AlexaFluor 700, CD80-PE/Cy7, CD86-PerCP/Cy5.5, CD40-PE, CD1b-APC, and CD1c-pacific blue (BioLegend) on ice for 45 min. Staining buffer and washing buffer was 1% BSA in 1x PBS. Stained cells were fixed in 2% paraformaldehyde in 1x PBS on ice for 15 min. Fixed cells were stored in staining buffer and read on Gallios Flow Cytometer (Beckman-Coulter, Brea, CA). For each sample, 30,000 to 50,000 events were assessed. Analysis was conducted with Kaluza flow cytometry analysis software (Beckman-Coulter).

Hwang S.A., Kruzel M.L, & Actor J.K. (2016). Recombinant human lactoferrin modulates human PBMC derived macrophage responses to BCG and LPS. Tuberculosis (Edinburgh, Scotland), 101 Suppl, S53-S62.

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Multiparametric Flow Cytometry Panel

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The following fluorochrome-labeled human monoclonal antibodies were used for flow cytometry analysis: CD3-PerCP-Cy™5.5 (SP34-2, BD Biosciences), CD4-PE/Cyanine7 (OKT4, BioLegend), CD8α-Brilliant Violet 711™ (SK1, BioLegend), CD8β- PE/Cyanine7 (SIDI8BEE, eBioscience), CD1a-FITC (NA1/34-HLK, Invitrogen), CD1b-APC (SN13, BioLegend), CD1c-APC (L161, BioLegend), CD1d-Brilliant Violet 421™ (51.1, BioLegend), CD11b-PE (ICRF44, BD Biosciences), CD11c-PE (3.9, BioLegend), CD14-PE (M5E2, BD Biosciences), CD20-PE (2H7, BioLegend), CD28-PE (CD28.2, BD Biosciences), CD31-Brilliant Violet 421™ (WM59, BioLegend), CD69 (FN50, BioLegend), HLA-ABC (G46-2.6, BD Biosciences), HLA-DR, DP, DQ (Tü39, BioLegend), CCR7-PE (G043H7, BioLegend), CXCR3-PE/Cyanine7 (G025H7, BioLegend), CD95-Brilliant Violet 510™ (DX2, BioLegend), CD159a (NKG2AC)-APC (Z199, Beckman Coulter), TCR Vβ F1- PE/Cyanine7 (8AC, Invitrogen), TCR Vα24-Jα18- APC/Cyanine7 (6B11, BioLegend), TCR Vα7.2-APC/Cyanine7 (3C10, BioLegend), PLZF-PE (Mags.21F7, eBioscience), EOMES-FITC (WD1928, eBioscience), and IL-4-Brilliant Violet 421™ (MP4-25D2, BioLegend).

Choi S.M., Park H.J., Choi E.A., Jung K.C, & Lee J.I. (2023). CD1b glycoprotein, a crucial marker of thymocyte development during T cell maturation in cynomolgus monkeys. Scientific Reports, 13, 14388.

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