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Rabbit polyclonal anti dsred

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Rabbit polyclonal anti-DsRed is a laboratory reagent produced by immunizing rabbits with the DsRed protein. The resulting antibodies can be used to detect and quantify the presence of DsRed in various experimental systems.

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Lab products found in correlation

Rabbit polyclonal anti cd4, by Atlas Antibodies (2 mentions) Vectashield, by Vector Laboratories (2 mentions) Rat monoclonal anti ncadherin, by Developmental Studies Hybridoma Bank (2 mentions) Goat polyclonal anti gfp, by Abcam (2 mentions) Rat monoclonal anti gfp, by BioLegend (2 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (2 mentions) Rabbit polyclonal anti ha, by Merck Group (1 mentions) Mouse monoclonal anti ha, by Merck Group (1 mentions) Monoclonal mouse anti phosphorylated h2a x, by BioLegend (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Tcs sp8 x white laser confocal microscope, by Leica (1 mentions) Alexa fluor 405, by Thermo Fisher Scientific (1 mentions) Chicken polyclonal anti gfp, by Aves Labs (1 mentions) Goat polyclonal anti hnf4a, by Santa Cruz Biotechnology (1 mentions) Triton x 100, by Merck Group (1 mentions) Sudan black b, by Merck Group (1 mentions) Goat anti guinea pig af488, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Sodium borohydride, by Merck Group (1 mentions)

Spelling variants of this product

Rabbit anti-DsRed (141 citations) Anti-DsRed (56 citations) Rabbit anti-DsRed polyclonal antibody (5 citations)

5 protocols using rabbit polyclonal anti dsred

1

Western Blotting and Immunofluorescence Antibody Protocols

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The following antibodies were used for western blotting experiments: monoclonal mouse anti-HA (1:1000, Sigma, St. Louis, MO, USA), rabbit polyclonal anti-dsRed (1:1000, Clontech Laboratories, Mountain View, CA, USA), polyclonal rabbit anti-HA (1:1000, Sigma, St. Louis, MO, USA), and monoclonal mouse anti-alpha-tubulin Ab-2 (DM1A) (1:10000, NeoMarkers, Fremont, CA). The following antibodies were used for immunofluorescence analysis: monoclonal mouse anti-HA (1:100, Sigma, St. Louis, MO, USA), rabbit polyclonal anti-dsRed (1:100, Clontech Laboratories, Mountain View, CA, USA), polyclonal rabbit anti-HA (1:100, Sigma, St. Louis, MO, USA), monoclonal mouse anti-Dmc1/Rad51, Clone 51RAD01 (1:50, NeoMarkers, Fremont, CA), and monoclonal mouse anti-phosphorylated H2A.X (1:200, BioLegend, San Diego, CA).
Ali E.I., Loidl J, & Howard-Till R.A. (2018). A streamlined cohesin apparatus is sufficient for mitosis and meiosis in the protist Tetrahymena. Chromosoma, 127(4), 421-435.
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2

Immunofluorescent Staining of Drosophila Eyes

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Pupal and adult eye–brain complexes were dissected in cold Schneider’s Drosophila medium and fixed in 4% paraformaldehyde in phosphate-buffered saline for 40 min. Tissues were washed in Phosphate-buffered saline + 0.4% Triton-X (PBST) and mounted in Vectashield (Vector Laboratories, CA). Images were obtained with a Leica TCS SP8-X white laser confocal microscope with a ×63 glycerol objective (c = 1.3). The primary antibodies used in this study with given dilutions were as follows: mouse monoclonal anti-Chaoptin (1:200; Developmental Studies Hybridoma Bank); rat monoclonal anti-nCadherin (1:100; Developmental Studies Hybridoma Bank); rabbit monoclonal anti-Atg8 (1:100; Abcam); mouse monoclonal anti-Trio (1:50; Developmental Studies Hybridoma Bank); mouse monoclonal anti-LAR (1:50; Developmental Studies Hybridoma Bank); goat polyclonal anti-GFP (1:1000; Abcam); rat monoclonal anti-GFP (1:500; BioLegend); rabbit polyclonal anti-CD4 (1:600; Atlas Antibodies); rabbit polyclonal anti-DsRed (1:500; ClonTech); rabbit anti-Syd-1 (1:500; gift from Sigrist Lab). The secondary antibodies Cy3, Cy5 (Jackson ImmunoResearch Laboratories) and Alexa488 (Invitrogen) were used in 1:500 dilution.
Kiral F.R., Linneweber G.A., Mathejczyk T., Georgiev S.V., Wernet M.F., Hassan B.A., von Kleist M, & Hiesinger P.R. (2020). Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring. Nature Communications, 11, 1325.
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3

Comprehensive Whole-Mount Imaging Techniques

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Whole-mount in situ hybridization was performed as previously described30 (link). We used following probes: prox1a, foxa3, sepp1b, cp, and fabp10a. Whole-mount immunostaining was performed as previously described31 (link), using the following antibodies: chicken polyclonal anti-GFP (1:1000; Aves Labs, Tigard, OR, USA), rabbit polyclonal anti-Prox1 (1:150; GeneTex, Irvine, CA, USA), mouse monoclonal anti-Anxa4 (also named as 2F11; 1:100; Abcam, Cambridge, MA USA), rabbit polyclonal anti-DsRed (1:200; Clontech, Mountain View, CA, USA), rabbit monoclonal anti-DsRed (1:400; Allele Biotechnology, San Diego, CA, USA), goat polyclonal anti-Hnf4a (1:50; Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-Bhmt (1:800; gift from Jinrong Peng at Zhejiang University, China), mouse monoclonal anti-Alcam (Zn5; 1:10; ZIRC, USA), and conjugated secondary antibodies, including Alexa Fluor 405, 488, 568, and 647 (1:500; Life Technologies, Grand Island, NY, USA).
Wu J., Choi T.Y, & Shin D. (2017). tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae. Gene Expression, 17(3), 237-249.
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4

Immunofluorescence Staining of Cell Cultures

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DAPI (4′, 6′-diamidino-2-phenlyindole, #D1306), Isolectin GS-IB4 (#I21411, #I21412) and 10X PBS (#70011) were purchased from Invitrogen (Carlsbad, CA). Sodium borohydride (#480886), 95% paraformaldehyde (#441244), ethanol (#E7148), Triton X-100 (#T8532) and Sudan Black B (#199664) were purchased from Sigma-Aldrich (St. Louis, MO). Chicken anti-GFP (1:2000; #AB13970) was purchased from Abcam (Cambridge, MA). Rabbit polyclonal anti-dsRed (1:1000; #632496) was purchased from Clontech (Mountain View, CA). Guinea pig anti-A2A adenosine receptor (1:4000; A2A-GP-Af1000) was purchased from Frontier (Hokkaido, Japan). Rabbit anti-phospho-ERK (1:1000; #9101) was purchased from Cell Signaling (Beverly, MA). Rabbit anti-Iba (1:1000; #019-19741) was purchased from Wako (Osaka, Japan). AF488 goat anti-chicken (1:1000; #A11039), AF488 goat anti-guinea pig (1:1000; #A11073) and AF568 goat anti-rabbit (1:1000; #A11011) were purchased from Invitrogen.
Kupferschmidt D.A., Cody P.A., Lovinger D.M, & Davis M.I. (2015). Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals. Frontiers in Neuroanatomy, 9, 6.
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5

Immunohistochemical Analysis of Drosophila Eye-Brain

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Immunohistochemistry and fixed imaging Pupal and adult eye-brain complexes were dissected in cold Schneider's Drosophila medium and fixed in 4% paraformaldehyde (PFA) in PBS for 40 minutes. Tissues were washed in PBST (0.4% Triton-X) and mounted in Vectashield (Vector Laboratories, CA). Images were obtained with a Leica TCS SP8-X white laser confocal microscope with a 63X glycerol objective (NA = 1.3). The primary antibodies used in this study with given dilutions were as follows: rat monoclonal anti-nCadherin (1:100; Developmental Studies Hybridoma Bank); goat polyclonal anti-GFP (1:1000; Abcam); rat monoclonal anti-GFP (1:500; BioLegend); rabbit polyclonal anti-CD4 (1:600; Atlas Antibodies); rabbit polyclonal anti-DsRed (1:500; ClonTech). The secondary antibodies Alexa488, Cy3, Cy5 (Jackson ImmunoResearch Laboratories) were used in 1:500 dilution.
Kiral F.R., Dutta S.B., Linneweber G.A., Hilgert S., Poppa C., Duch C., von Kleist M., Hassan B.A, & Hiesinger P.R. (2021). Brain connectivity inversely scales with developmental temperature in Drosophila. Cell reports, 37(12).
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