P47phox

Primary antibodies against gp91phox/NOX2, p47phox, acetylated α-tubulin, p65, IkB, c-fos, and c-jun were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Total-ERK, total-p38, total-JNK, phosphor-ERK, phosphor-p38, phosphor-JNK, β-actin, and HDAC6 were purchased from Cell Signaling Technology (Beverly, MA, U.S.A.). Hindsiipropane B was synthesized from commercially available starting materials with the Wittig-Horner reaction, Claisen-Schmidt condensation and hydrogenation as key steps (14 (link)). 10 mM stock solution of hindsiipropane B was prepared in dimethyl sulfoxide. Oligonucleotide primers (HDAC6, CCL2, CXCL8, CXCL10, Nox2, p22phox, p47phox, and β-actin) (Bioneer, Seoul, Korea) were obtained from commercially.

N-acetyl cysteine (NAC), diphenylene iodonium (DPI), apocynin (APO), and tubastatin A (TBA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dihydroethidium (DHE) was purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). Primary antibodies against gp91phox/NOX2, p47phox, acetylated α-tubulin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and those against HDAC6 and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Lipofectamine 3000 transfection reagent was purchased from Invitrogen (Carlsbad, CA, USA). A luciferase assay kit was purchased from Promega (Madison, WI, USA). Oligonucleotide primers (HDAC6, CCL2, CXCL8, CXCL10, Nox2, p22phox, p47phox, and β-actin) and HDAC6 siRNA were obtained from Bioneer (Seoul, Korea).