Dulbecco’s modified Eagle’s medium (DMEM), GlutaMAX, sodium pyruvate and non-essential amino acids were obtained from Life Technologies. Fatty acid-free, ultra-pure BSA and protease inhibitor cocktail were obtained from Roche. BCS, copper chloride, 8-bromo-cAMP, dibutyryl cAMP, IBMX, isoproterenol, and free glycerol reagent were obtained from Sigma-Aldrich. BDH Aristar Ultra concentrated nitric acid was obtained from VWR. NEFA kits were obtained from Wako. H-89 dihydrochloride, cilostamide, and rolipram were obtained from EMD Biosciences. CAY10499 and cyclic AMP assay kits were obtained from Cayman Chemical. Restriction endonucleases, Phusion High-Fidelity DNA polymerase, T4 DNA ligase, and Gibson Assembly Master Mix were obtained from New England Biolabs. TaKaRa ExTaq DNA polymerase was purchased from Clontech. Primers were purchased from Integrated DNA Technologies. All other materials used in biochemical assays were purchased from Sigma unless otherwise noted. Baculoviral particles were generated using the BaculoGold™ transfection kit from BD Biosciences. All DNA constructs were verified by sequencing by Quintara Biosciences, and all plasmids used for transfections were prepared using the EndoFree Maxi Kit (Qiagen).
Cyclic amp assay kit
Manufactured by Cayman Chemical
Cyclic AMP assay kits are laboratory tools used to quantify the levels of cyclic adenosine monophosphate (cAMP) in biological samples. cAMP is an important second messenger molecule involved in various cellular signaling pathways. These kits provide a standardized and reliable method for measuring cAMP concentrations, which can be useful in research and drug discovery applications.
Automatically generated - may contain errors
Lab products found in correlation
3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (2 mentions)
Dibutyryl camp, by Merck Group (2 mentions)
Copper chloride, by Merck Group (2 mentions)
Bovine calf serum (bcs), by Merck Group (2 mentions)
Gibson assembly master mix, by New England Biolabs (2 mentions)
Takara ex taq dna polymerase, by Takara Bio (2 mentions)
Free glycerol reagent, by Merck Group (2 mentions)
T4 dna ligase, by New England Biolabs (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Nefa kit, by Fujifilm (2 mentions)
Restriction endonuclease, by New England Biolabs (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Isoproterenol, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
Phusion high fidelity dna polymerase, by New England Biolabs (2 mentions)
Endofreemaxi kit, by Qiagen (2 mentions)
8 bromo camp, by Merck Group (2 mentions)
Bdh aristar ultra concentrated nitric acid, by Avantor (2 mentions)
Cay10499, by Cayman Chemical (2 mentions)
Baculogold transfection kit, by BD (2 mentions)
4 protocols using cyclic amp assay kit
1
Analysis of Lipolysis Signaling Pathway
2
Analysis of Lipolysis Signaling Pathway
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Dulbecco’s modified Eagle’s medium (DMEM), GlutaMAX, sodium pyruvate and non-essential amino acids were obtained from Life Technologies. Fatty acid-free, ultra-pure BSA and protease inhibitor cocktail were obtained from Roche. BCS, copper chloride, 8-bromo-cAMP, dibutyryl cAMP, IBMX, isoproterenol, and free glycerol reagent were obtained from Sigma-Aldrich. BDH Aristar Ultra concentrated nitric acid was obtained from VWR. NEFA kits were obtained from Wako. H-89 dihydrochloride, cilostamide, and rolipram were obtained from EMD Biosciences. CAY10499 and cyclic AMP assay kits were obtained from Cayman Chemical. Restriction endonucleases, Phusion High-Fidelity DNA polymerase, T4 DNA ligase, and Gibson Assembly Master Mix were obtained from New England Biolabs. TaKaRa ExTaq DNA polymerase was purchased from Clontech. Primers were purchased from Integrated DNA Technologies. All other materials used in biochemical assays were purchased from Sigma unless otherwise noted. Baculoviral particles were generated using the BaculoGold™ transfection kit from BD Biosciences. All DNA constructs were verified by sequencing by Quintara Biosciences, and all plasmids used for transfections were prepared using the EndoFree Maxi Kit (Qiagen).
3
Quantification of Cellular cAMP Levels
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Cyclic AMP Assay Kit (Cayman Chemical, Ann Arbor, MI) was employed to quantitate cAMP according to manufacturer’s protocol. To determine effect of miR-200c and PDE7B knockdown on cellular cAMP concentration, cells were transfected with control, miR-200c mimic or PDE7B siRNAs for 4 days prior to the analysis of cAMP.
4
Quantification of Cellular cAMP Levels
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