E el h0102c

Manufactured by Elabscience

Sourced in China

The E-EL-H0102c is a compact and precise pH meter designed for laboratory use. It features a digital display and a built-in temperature compensation function. The device is capable of measuring pH values within a range of 0.00 to 14.00 pH.

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Lab products found in correlation

11 protocols using e el h0102c

Quantifying CMV Antibodies and Inflammatory Markers

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Enzyme-linked immunosorbent assay kit (CMV IgG antibody Diagnostic Kit or CMV IgG antibody Diagnostic Kit, Haitai Biotech Inc, Zhuhai, China) was employed to determine CMV infection (IgG antibody, IgM antibody) using baseline frozen (-80°C) serum samples according to the product specifications (antibody titer <1.1 is seronegative; antibody titer ≥1.1, seropositive). The test sensitivity was 96.2 and 99.9% and the specificity was 97.8 and 99.8% for CMV IgG and IgM, respectively. The optical density (OD) value and the antibody titer were proportional. Among the 563 participants, 16 participants were CMV IgG seronegative (antibody titer <1.1) and 547 were CMV IgG seropositive (≥1.1); 561 participants were CMV IgM seronegative (<1.1) and 2 were CMV IgM seropositive (≥1.1). All participants were further categorized into four groups according to quartiles of CMV antibody concentrations (U): 0–3.75, 3.76–4.25, 4.26–4.85, and >4.85.
We also evaluated CRP, TNF-α, and IL-6 levels with E-EL-H0043c, E-EL-H0109c, and E-EL-H0102c, respectively (Wuhan Elabscience Biotechnology, Wuhan, China), according to the manufacturer’s instructions. The OD value and the CRP/TNF-α/IL-6 concentration were proportional. CRP/TNF-α/IL-6 concentrations in the samples were calculated based on the OD value and standard curve, according to the kit manual, using the software curve expert 1.3.

Li Z., Tang Y., Tang N., Feng Q., Zhong H., Liu Y.M., Wang L.M, & He F. (2017). High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population. PLoS ONE, 12(8), e0181440.

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Quantifying CIRP and Inflammatory Factors

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The CIRP concentrations in the human and rat serum were detected using CIRP ELISA kits (SEG886Hu and SEG886Ra, Cloud-Clone Corp.) according to the manufacturer's protocol. The concentration difference of CIRP in the human serum between the two time points was recorded as ΔCIRP. The ELISA kits for interleukin 6 (IL-6, E-EL-H0102c and E-EL-R0015c, Elabscience), IL-1β (E-EL-H0149c and E-EL-R0012c, Elabscience), and tumor necrosis factor alpha (TNF-α, E-EL-H0109c and E-EL-R2856c, Elabscience) were used to detect inflammatory factors in the rat serum and the cell culture medium.

Zheng X., Fan Y., Li J., Ma T., Li Y., Wang Q., Yan Y, & Liu W. (2022). Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury. Oxidative Medicine and Cellular Longevity, 2022, 3576892.

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Serum Cytokine Profiling from Blood

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Five milliliters of venous blood was drawn from each subject and kept standing for 30 minutes. The supernatant was collected after centrifugation at 3000 rpm for 20 minutes, and the blood cells in the pellet were used for genome extraction. The extracted supernatant was stored at −80°C for serum cytokine detection by enzyme linked immunosorbent assay (ELISA). The TNF-α-detection kit used was E-EL-H0109c (Elabscience, Wuhan, China), and the IL-6-detection kit used was E-EL-H0102c (Elabscience, Wuhan, China).

Zhang Y., Li M., Bao L, & Hu P. (2019). A case-control study on the relationship between miRNAs single nucleotide polymorphisms and sepsis risk. Medicine, 98(33), e16744.

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Cytokine Profiling in A549 Cells

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A549 and H/R-A549 cells (5x10³/well) were inoculated into 96-well plates and treated with different MAF concentrations (5, 10 and 20 µΜ) for 24 h at 37˚C. Subsequently, the cell culture medium was centrifuged at 2,000 x g for 4 min at 4˚C to remove debris and the supernatant was collected for assay. The levels of IL-6, IL-1β and IL-10 in cells were quantified using their corresponding ELISA kits (cat. no. E-EL-H0102c, E-EL-H0149c and E-EL-H0103c; Elabscience Biotechnology Co., Ltd.) according to the manufacturer's protocols. The absorbance at 450 nm was measured using a microplate reader (Thermo Fisher Scientific, Inc.).

Chen X, & Huang J. (2021). Mangiferin inhibits hypoxia/reoxygenation-induced alveolar epithelial cell injury via the SIRT1/AMPK signaling pathway. Experimental and Therapeutic Medicine, 22(5), 1220.

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Quantifying Secretion Mechanisms in LPS-Stimulated HMECs

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The levels of human (SEC642Hu) and mouse serum MSN (SEC642Mu) and PCT (SEA689Mu) were quantified by ELISA using specific kits (USCN Life Sciences, Wuhan, China), according to the manufacturer's instructions. To explore the secretion mechanisms of MSN in HMECs after LPS stimulation, three inhibitors of the main pathways of protein secretion were employed in the experiment. The dosage of inhibitors is as follows: the autophagy inhibitor 3-methyladenine (3-MA, 1 mM, Selleck, S2767), the exosome secretion inhibitor 5-(N,N-dimethyl)-amiloride DMA (DMA, 50 nM, APExBIO, C3505), and the protein transport inhibitor brefeldin A (BFA, 10 ng/ml, APExBIO, B1400). The supernatants were collected from the cultured medium 24 h later. To explore the role of MSN in the process of excessive inflammation caused by sepsis, HMECs were, respectively, transfected with siNC and siMSN RNA for 48 h, then exposed to LPS for 24 h, and the supernatants were collected to measure IL-1β, IL-18, IL-6, and TNF-α levels (E-EL-H0149c, IL-18 E-EL-H0253c, E-EL-H0102c, E-EL-H0109c, Elabscience Biotechnology, Wuhan, China).

Chen Y., Wang J., Zhang L., Zhu J., Zeng Y, & Huang J.A. (2021). Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis. Journal of Immunology Research, 2021, 6695679.

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ELISA Assay of Inflammatory Cytokines

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ELISA analysis was performed as previously described (Yan et al., 2021 (link)). The inflammatory cytokines TNF-α, IL-6 and IL-1β in brain tissue or cell culture medium were detected with ELISA kits (Elabscience Biotechnology, E-EL-M0037c for mouse IL-1β, E-EL-H0149c for human IL-1β, E-EL-M0049c for mouse TNF-α, E-EL-H0109c for human TNF-α, E-EL-M0044c for mouse IL-6, E-EL-H0102c for human IL-6). All procedures were performed according to the manufacturer’s instructions.

Zeb S., Ye H., Liu Y., Du H.P., Guo Y., Zhu Y.M., Ni Y., Zhang H.L, & Xu Y. (2023). Necroptotic kinases are involved in the reduction of depression-induced astrocytes and fluoxetine’s inhibitory effects on necroptotic kinases. Frontiers in Pharmacology, 13, 1060954.

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Quantifying Inflammatory Cytokine Levels

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The levels of inflammatory factors, including IL-1, IL-6, and IFN-γ, were determined by a commercial ELISA kit (E-EL-H0088c, E-EL-H0102c, E-EL-H0150c, ElabScience, China). All experiments were conducted according to the manufacturer's protocol.

Zhang J., Zhang D, & Zhao J. (2022). CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages. Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 61(6).

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Quantifying IL-6 and IL-10 Cytokines

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Both IL-6 and IL-10 were detected by ELISA, and the detection kits were purchased from Wuhan Elabscience Biotechnology Co., Ltd., with the item numbers of E-EL-H0102c and E-EL-H0103c, respectively. Refer to the kit instructions for specific detection steps.

Peng L., Du B., Sun L., Zhao Y, & Zhang X. (2019). Short-term efficacy and safety of prednisone in herpes zoster and the effects on IL-6 and IL-10. Experimental and Therapeutic Medicine, 18(4), 2893-2900.

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Sebocyte Cytokine Response to PM2.5

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SZ95 sebocytes were seeded in 24-well plates (1×10⁵ cells/well). After 48-h exposure to different concentrations of PM2.5 suspension, the supernatant was collected at 4°C, centrifuged for 20 min at 1,000 × g at 4°C, and the supernatant was collected and stored at −20°C for subsequent assays. Concentrations of IL-1α, IL-6 and IL-8 were determined using commercial ELISA kits (E-EL-H0088c, E-EL-H0102c and E-EL-H0048c; ElabScience Biotechnology Co., Ltd., Wuhan, China) according to the manufacturer's instructions.

Liu Q., Wu J., Song J., Liang P., Zheng K., Xiao G., Liu L., Zouboulis C.C, & Lei T. (2017). Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes. International Journal of Molecular Medicine, 40(4), 1029-1036.

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Quantification of IL-6 and CCL2 in Cell Culture Media

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The levels of interleukin-6 (IL-6) and C-C motif chemokine ligand 2 (CCL2) in the conditioned medium were determined using the ELISA reagent kits purchased from Elabscience (IL-6: E-EL-H0102c; CCL2: E-EL-H6005) following the manufacturer’s instructions. Briefly, the cell culture supernatants were collected and centrifuged at 1000 g for 20 min to eliminate cell debris. Then, 100 μL conditioned medium was added to the appropriate wells and incubated at 37 °C for 90 min. Then, the liquid was decanted, the biotinylated detection antibody working solution was added to the wells, and the mixture was incubated at 37 °C for 60 min. The wells were washed 3 times with wash buffer, the HRP conjugate working solution was added, and the wells were incubated at 37 °C for 30 min. Then, the wells were washed 5 times with wash buffer, and the substrate reagent was added for 15 min. The stop solution was added to the wells, and the OD values were determined at 450 nm.

Liu L., Cui J., Zhao Y., Liu X., Chen L., Xia Y., Wang Y., Chen S., Sun S., Shi B, & Zou Y. (2021). KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1. Molecular Cancer, 20, 77.

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