Ammonium persulfate aps

NEB buffer 4 and Nt.BbvCI endonuclease were obtained from New England BioLabs (Ipswich, MA, USA), and the polymerase Klenow fragment exo- was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Streptavidin-coated 96-well plates were purchased from BEAVER Nano-Technologies (Suzhou, China). 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), Tween 20, and invertase from baker’s yeast were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethylene glycol, bis(aminoethyl ether)-N, N, N’, N’ tetraacetic acid (EGTA), glycerol, sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (sulfo-SMCC), bovine serum albumin (BSA), deoxynucleotide triphosphate (dNTP) solution mixture, 40% acrylamide mix solution, ammonium persulfate (APS), and 1,2-bis(dimethylamino)-ethane (TEMED) were obtained from Sangon Biotechnology Co. Ltd. (Shanghai, China). All other chemicals were of analytical grade and were used as received without purification. All water used in this work was RNase-free.
The oligonucleotides used in this assay (Table 1) were synthesized and purified by Sangon Biotechnology Co. Ltd. (Shanghai, China). Buffer I (0.1 M NaCl, 0.1 M sodium phosphate buffer, pH 7.3, 0.05% Tween-20) was used throughout the experiment.

Monodispersed Magnetic Streptavidin Microspheres (MBs) were purchased from Tianjin Bessel Chromatography Technology Development Center (Tianjin, China), with a solid content of 0.5% (w/v) and a particle size of 300 nm. ZEN, Ochratoxin a (OTA), Fumonisin (FB1), Aflatoxin B1 (AFB1) and Aflatoxin M1 (AFM1) were bought form Meizheng Testing Technology Co., Ltd. (Beijing, China). EnGen LbaCas12a from Lachnospiraceae bacterium ND2006 was purchased from New England Biolabs (Ipswich, MA, USA). CRISPR RNA (crRNA) and other ssDNAs were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Sequence details are given in Table S1. The buffer solution 1 was used as a reaction solution for the streptavidin-modified magnetic beads and DNA. The CutSmart Buffer and NEBuffer™ 2.1 was from New England Biolabs. Buffer components are visible in Table S2. PBS, agarose, 50 × TAE, 5 × TBE, EDTA, 30% acrylamide solution (ACR-BIS, 29:1), tetramethylethylenediamine (TEMED), PAGE coagulant, ammonium persulfate (APS)⁺, 6× loading buffer and DNA Marker were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). RNase-free water was employed as the solvent throughout the experiment. All chemical reagents were of analytical grade.

All the oligonucleotides used in this study (Table S1†) were synthesized and purified by Sangon Biotechnology Co. Ltd. (Shanghai, China), the modified sequences were HPLC purified, and other sequences without modification were ultraPAGE purified. A telomerase ELISA kit was obtained from Innovation Beyond Limits (Germany). Glycerol, deoxynucleotide triphosphates (dNTPs) solution mixture, 40% acrylamide mix solution, ammonium persulfate (APS), and 1,2-bis(dimethylamino)-ethane (TEMED) were obtained from Sangon Biotechnology Co. Ltd. (Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from KeyGen Biotech. Co. Ltd. (Nanjing, China). 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), Tween 20 and epigallocatechingallate (EGCG) were bought from Sigma-Aldrich (St. Louis, MO, USA). The transfection reagent, X-tremeGENE, was bought from Roche. All of the chemicals were of analytical grade and were used as received without any purification.