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Immage 800 immunochemistry system

Manufactured by Beckman Coulter
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The Immage 800 Immunochemistry System is a diagnostic instrument designed for the quantitative determination of analytes in biological samples. It utilizes immunoassay technology to measure the concentration of specific proteins, hormones, or other molecules in the sample.

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Lab products found in correlation

Vi cell xr viability analyzer, by Beckman Coulter (2 mentions) Fetuin a, by R&D Systems (1 mentions) Au5800 clinical chemistry system, by Beckman Coulter (1 mentions) Monoclonal antibodies against, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Advia 2400 chemistry system, by Siemens (1 mentions) D glucose, by Merck Group (1 mentions) Facscalibur, by BD (1 mentions) Cobas e601, by Roche (1 mentions) Hscrp reagent, by Beckman Coulter (1 mentions) Dxc 800 analyzer, by Beckman Coulter (1 mentions)

19 protocols using immage 800 immunochemistry system

1

Measuring Candidate Biomarker Proteins

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Protein concentrations of previously published candidate biomarkers were measured by ELISA. YKL-40 (Sunred Biological Technology Co) and Fetuin-A (α2-Heremans-Schmid glycoprotein, R&D systems) measurements were performed according to manufacturer’s instructions. Haptoglobin concentrations were determined by standard immunonefolometry, using a immage 800 immunochemistry system (Beckman Coulter Inc.). Thrombocyte counts were measured on a CD4000 impedance hematology analyzer.
van Linde M.E., van der Mijn J.C., Pham T.V., Knol J.C., Wedekind L.E., Hovinga K.E., Aliaga E.S., Buter J., Jimenez C.R., Reijneveld J.C, & Verheul H.M. (2016). Evaluation of potential circulating biomarkers for prediction of response to chemoradiation in patients with glioblastoma. Journal of Neuro-Oncology, 129, 221-230.
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2

Characterization of Stable CHO Cell Pools

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The stably transfected CHO cell pools expressing different targeting vectors were generated via RMCE (Fig. 1A). They were characterized for growth and titre in 14-day fed-batch cultures in 50 tubespins (TPP) in the humidified Kuhner shaker (Adolf Kühner AG). Cell density, viability and antibody titre were monitored at day 3, 5, 7, 9, 11 and 14 using the Vi-Cell XR viability analyzer (Beckman Coulter) and IMMAGE 800 immunochemistry system (Beckman Coulter), respectively. The detailed protocols for generation and characterization of stable pools were described in Supplementary Materials.
Ong H.K., Nguyen N.T., Bi J, & Yang Y. (2022). Vector design for enhancing expression level and assembly of knob-into-hole based FabscFv-Fc bispecific antibodies in CHO cells. Antibody Therapeutics, 5(4), 288-300.
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3

Diabetic Rat Urine and Blood Analysis

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Before BOLD-MRI, rats from the randomly chosen DM group were placed in metabolic cages for 24 h to collect 24-h urine samples. The urine albumin concentration was determined using a Beckman Coulter IMMAGE® 800 Immunochemistry system and associated reagents (Beckman Coulter, Brea, CA, USA) according to the manufacturer’s instructions, and then multiplied by 24 h urine volume to get 24 h urinary albumin excretion. Blood creatinine and urea nitrogen were tested from tail vein blood samples using a Beckman Coulter AU5800 Clinical Chemistry System (Beckman Coulter) according to the manufacturer’s instructions, and this group was euthanized for histopathological examination, as previously stated.
Wang Q., Guo C., Zhang L., Zhang R., Wang Z., Xu Y, & Xiao W. (2018). BOLD MRI to evaluate early development of renal injury in a rat model of diabetes. The Journal of International Medical Research, 46(4), 1391-1403.
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4

Immunoglobulin Levels in Ankylosing Spondylitis

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Sera from all studied subjects were collected on the day when phenotypical studies were done, and stored at −80°C. Concentrations of IgG, IgA and IgM were subsequently examined in sera of AS/nb, AS/b patients and HC by nephelometry in an Immage 800 Immunochemistry System (Beckman Coulter, Brea, CA, U.S.A). For AS/b patients, determinations of Igs were also performed in serum that had been collected just before initiation of treatment with TNF blockers.
Bautista-Caro M.B., Arroyo-Villa I., Castillo-Gallego C., de Miguel E., Peiteado D., Plasencia-Rodríguez C., Villalba A., Sánchez-Mateos P., Puig-Kröger A., Martín-Mola E, & Miranda-Carús M.E. (2014). Decreased Frequencies of Circulating Follicular Helper T Cell Counterparts and Plasmablasts in Ankylosing Spondylitis Patients Naïve for TNF Blockers. PLoS ONE, 9(9), e107086.
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5

Serum Beta-2-Microglobulin Quantification in MM

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Serum samples of treated MM patients were taken after a 12–14 h overnight fasting simultaneously with the PB and BM samples obtained for flow cytometric analysis. Serum beta2-microglobulin (B2-M) was measured using latex particle-enhanced turbidimetry kit (Dako Beta-2-Microglobulin PET Kit, Code No. 0052) on the IMMAGE 800 Immunochemistry System (Beckman Coulter, USA).
Batorov E.V., Aristova T.A., Sergeevicheva V.V., Sizikova S.A., Ushakova G.Y., Pronkina N.V., Shishkova I.V., Shevela E.Y., Ostanin A.A, & Chernykh E.R. (2020). Quantitative and functional characteristics of circulating and bone marrow PD-1- and TIM-3-positive T cells in treated multiple myeloma patients. Scientific Reports, 10, 20846.
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6

Comprehensive Immunological Profiling

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Serum levels of immunoglobulin G, A, and M and C-reactive protein (in g/L) were assessed by automated nephelometry using an Immage 800 Immunochemistry System (Beckman Coulter). Serum autoantibodies, ANCA, RF and anti-cardiolipin were detected using the ANA test (BioRad, Philadelphia, PA), ANCA test (Inova Diagnostics, San Diego, CA), and ACA test (Orgentec Diagnostic, Mainz, Germany), respectively. Lymphocyte subsets were enumerated by flow cytometry using FACS CANTO II (BD Bioscience, Franklin Lakes, NJ) and were subsequently analyzed using FlowJo software (Tree Star, Ashland, OR, USA). Monoclonal antibodies against CD3, CD4, CD8, CD16, CD19 and HLA-DR were purchased from BD Biosciences.
Podrazil M., Horvath R., Becht E., Rozkova D., Bilkova P., Sochorova K., Hromadkova H., Kayserova J., Vavrova K., Lastovicka J., Vrabcova P., Kubackova K., Gasova Z., Jarolim L., Babjuk M., Spisek R., Bartunkova J, & Fucikova J. (2015). Phase I/II clinical trial of dendritic-cell based immunotherapy (DCVAC/PCa) combined with chemotherapy in patients with metastatic, castration-resistant prostate cancer. Oncotarget, 6(20), 18192-18205.
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7

Dried Blood Spot AAT Measurement

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The AAT level measurements were performed on dried blood spot (DBS) samples by a rate immune nephelometric method (Dade-Behring BN II, Germany and Immage 800 Immunochemistry System - (Beckman-Coulter, USA) [9 (link), 22 (link)].
Zhumagaliyeva A., Ottaviani S., Greulich T., Gorrini M., Vogelmeier C., Karazhanova L., Nurgazina G., DeSilvestri A., Kotke V., Barzon V., Zorzetto M., Corsico A, & Ferrarotti I. (2017). Case-finding for alpha1-antitrypsin deficiency in Kazakh patients with COPD. Multidisciplinary Respiratory Medicine, 12, 23.
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8

BALf Immunonephelometric AAT Quantification

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AAT was measured in BALf by a rate immune nephelometric method (Immage 800 Immunochemistry System, Beckman-Coulter, Brea, CA, USA).
Cagnone M., Piloni D., Ferrarotti I., Di Venere M., Viglio S., Magni S., Bardoni A., Salvini R., Fumagalli M., Iadarola P., Martinello S, & Meloni F. (2019). A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients. High-Throughput, 8(1), 5.
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9

Serum Complement and hs-CRP Measurement

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The levels of serum complement 3 (C3) and complement 4 (C4) were measured on IMMAGE 800 Immunochemistry System (Beckman Coulter, Brea, CA, USA). High-sensitivity CRP (hs-CRP) level was measured on ADVIA 2400 Chemistry System (Siemens Healthineers, Erlangen, Germany).
Liu X., Fan K., Lin Q., Tang M., Wang Q., Huang E., Zhang W., Chen T, & Ou Q. (2022). Serum-Derived Exosomal miR-140-5p as a Promising Biomarker for Differential Diagnosis of Anti-NMDAR Encephalitis With Viral Encephalitis. Frontiers in Immunology, 13, 840003.
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10

Quantitative A1AT Analysis via Nephelometry

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The A1AT quantitative analysis was performed using an immune nephelometric method (Immage 800 Immunochemistry System, Beckman-Coulter, USA) with commercially available reagents containing goat anti-human A1AT antibody (Beckamn-Coulter, USA). The nephelometer automatically dilutes analysed samples 1:216 to achieve optimal antigen-antibody equilibrium in the assay. The normal range of the instrument for A1AT in serum samples is 88–174 mg/dL with a cutoff value of 120 mg/dL [19 (link)].
Ferrarotti I., Poplawska-Wisniewska B., Trevisan M.T., Koepke J., Dresel M., Koczulla R., Ottaviani S., Baldo R., Gorrini M., Sala G., Cavallon L., Welte T., Chorostowska-Wynimko J., Luisetti M, & Janciauskiene S. (2015). How Can We Improve the Detection of Alpha1-Antitrypsin Deficiency?. PLoS ONE, 10(8), e0135316.
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