Navitoclax was synthesized at AbbVie, Inc. (North Chicago, IL). FDA-approved drug library was purchased from Enzo (Farmingdale, NY). Albendazole, mebendazole, oxibendazole, and oxfendazole were purchased from Sigma (St. Louis, MO). All siRNA pools were purchased from Dharmacon (Lafayette, CO). Noxa antibody was purchased from Abcam. Bim and Mcl-1 antibodies were purchased from Epitomics (Burlingame, CA). Caspase 3 and caspase 9 inhibitors were purchased from SantaCruz Biotechnology (Santa Cruz, CA). All the branched DNA reagents were purchased from Affymetrix (Santa Clara, CA).
Oxfendazole
Manufactured by Merck Group
Sourced in Italy, United States
Oxfendazole is a laboratory equipment product manufactured by Merck Group. It is a benzimidazole compound used as an anthelmintic agent for the treatment of gastrointestinal nematode infections in various animal species.
Automatically generated - may contain errors
Lab products found in correlation
Albendazole, by Merck Group (3 mentions)
Mebendazole, by Merck Group (2 mentions)
Oxibendazole, by Merck Group (1 mentions)
Navitoclax, by Abbvie (1 mentions)
Fda approved drug library, by Enzo Life Sciences (1 mentions)
Sirna pool, by Horizon Discovery (1 mentions)
Lc 101 oven, by Waters Corporation (1 mentions)
Waters 484 detector, by Waters Corporation (1 mentions)
Hplc grade solvent, by Merck Group (1 mentions)
Thiabendazole, by Merck Group (1 mentions)
Fenbendazole, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions)
4 protocols using oxfendazole
1
Navitoclax Drug Screening Protocol
2
Enantioselective HPLC Separation of Ricobendazole and Oxfendazole
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ricobendazole, oxfendazole and HPLC-grade solvents were purchased from Sigma-Aldrich (Milan, Italy) and used without further purification. HPLC enantioseparations were performed by using stainless-steel Chiralpak IG (250 mm × 10 mm I.D.) columns (Chiral Technologies Europe, Illkirch, France). The HPLC apparatus for semipreparative separations consisted of a Perkin-Elmer (Norwalk, CT, USA) 200 LC pump equipped with a Rheodyne (Cotati, CA, USA) injector, a 5000 μL sample loop, a Perkin-Elmer LC 101 oven and a Waters 484 detector (Waters Corporation, Milford, MA, USA). Data were processed using Clarity software (DataApex, Prague, Czech Republic).
3
Benzimidazole Exposure in C. elegans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C. elegans stock strains were maintained on NGM agar plates with OP50 E. coli as the food source as described (Brenner, 1974 (link)). Three biological replicates of approximately 20,000 L1 larvae C. elegans CB3474 ben-1(e1880)III were cultured in NGM media with E. coli OP50 at 1% (w/vol) at 20 °C for approximately 70 h until the adult stage, concentrations were kept consistent with micro-array experiment performed previously (Laing et al., 2010 (link)). Cultures were exposed to 1.13 mM of either albendazole (Sigma Cat# A4673), mebendazole (Sigma Cat# M2523), thiabendazole (Sigma Cat#T8904), oxfendazole (Sigma Cat#34176), fenbendazole (Sigma Cat# F5396) dissolved in DMSO as well as a DMSO negative control solution of for 4 h, care was taken to ensure that DMSO concentration were less than 1%. The worms were then harvested by centrifugation at 300xg for 3 min, no brake, and cleaned on a sucrose gradient, the supernatant was decanted and the pellet stored in TRIzol (Invitrogen # 15596-018) and immediately frozen at −80 °C until processing.
4
Oxfendazole and Albendazole Analytical Methods
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!