Human coronary arterial smooth muscle cells (HCASMC) and human aortic endothelial cells (HAEC) were purchased from Kurabo (Osaka, Japan). HCASMC were cultured routinely in Humedia-SB2 (Kurabosupplemented with 10% fetal calf serum (FCS, Life Technologies, Grand Island, NY) using plastic culture flasks (Corning, NY). HAEC were cultured routinely in M199 medium (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% FCS and 10 ng/mL of bFGF (Pepro Tech, Inc.) using collagen coated culture flasks (Iwaki, Chiba, Japan).
Plastic culture flasks
Manufactured by Corning
Sourced in United States
Plastic culture flasks are laboratory equipment designed for the growth and cultivation of cells, tissues, and microorganisms. They provide a sterile, controlled environment for culturing a variety of biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Yh 13, by Health Science Research Resources Bank (3 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium, by Nissui Pharmaceutical (3 mentions)
U 251mg, by Nissui Pharmaceutical (2 mentions)
A 172, by Health Science Research Resources Bank (2 mentions)
Am 38, by Health Science Research Resources Bank (2 mentions)
U 251mg, by Health Science Research Resources Bank (2 mentions)
U87mg, by American Type Culture Collection (2 mentions)
U138mg, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Nacalai Tesque (2 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Nissui Pharmaceutical (1 mentions)
Perampanel, by Eisai (1 mentions)
Dulbecco s modified eagle s minimum essential medium dmem, by Nissui Pharmaceutical (1 mentions)
Dmem medium, by Nacalai Tesque (1 mentions)
Escherichia coli, by Transgene (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Sk mel 28, by American Type Culture Collection (1 mentions)
Dtx 880 multimode detector, by Beckman Coulter (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
13 protocols using plastic culture flasks
1
Culturing Human Vascular Cells
2
Characterization of Human Glioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human malignant glioma cell lines A-172 (cell no. JCRB0228; lot no. 021999), AM-38 (cell no. IFO50492; lot no. 12082003), T98G (cell no. IFO50303; lot no. 1007), U-251MG (cell no. IFO50288; lot no. 12132002), and YH-13 (cell no. IFO50493; lot no. 1164) were purchased from Health Science Research Resources Bank (Sennan, Osaka, Japan). U-87MG (glioblastoma of unknown origin; cat. no. HTB-14; lot no. 2497162) and U-138MG (cat. no. HTB-16; lot no. 1104428) were purchased from the American Type Culture Collection (Manassas, VA, USA). In a previous study, we confirmed that O6-methylguanine-DNA methyltransferase (MGMT, a key factor of alkylating agents) is expressed in T98G, U-138MG and YH-13 cells by real-time RT-PCR and western blot analysis (29 (link)). Consistent with an earlier study (30 (link)), it was also confirmed that T98G (237 Met→Ile) and U-251MG (273 Arg→His) have a point mutation in the p53 gene (data not shown).
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM; Nissui Pharmaceutical Co., Ltd.) supplemented with 10% fetal calf serum (FCS; Life Technologies; Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C-humidified incubator with 5% CO2. Natural-type IFN-β (Toray Industries, Inc.) and TRAIL (Wako Pure Chemical Industries, Ltd.) were employed for the experiments.
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM; Nissui Pharmaceutical Co., Ltd.) supplemented with 10% fetal calf serum (FCS; Life Technologies; Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C-humidified incubator with 5% CO2. Natural-type IFN-β (Toray Industries, Inc.) and TRAIL (Wako Pure Chemical Industries, Ltd.) were employed for the experiments.
3
Mechanisms of Ribavirin Sensitivity in Gliomas
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To elucidate the mechanisms of ribavirin sensitivity in malignant gliomas, we used two types of malignant glioma cell lines (U-87MG and U-138MG) which have different MGMT mRNA and MGMT protein expression.
The human malignant glioma U-87MG and U-138MG cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). These cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Nissui Pharmaceutical, Tokyo, Japan) containing 10% fetal calf serum (FCS; Life Technologies; Thermo Fischer Scientific, Grand Island, NY, USA) using plastic culture flasks (Corning, NY, USA) in a standard humidified incubator at 37°C with an atmosphere of CO2.
The human malignant glioma U-87MG and U-138MG cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). These cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Nissui Pharmaceutical, Tokyo, Japan) containing 10% fetal calf serum (FCS; Life Technologies; Thermo Fischer Scientific, Grand Island, NY, USA) using plastic culture flasks (Corning, NY, USA) in a standard humidified incubator at 37°C with an atmosphere of CO2.
4
Culturing Human Gastric Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The poorly differentiated human gastric adenocarcinoma cell line MKN45 was used in the present study. Cells were grown in plastic culture flasks (Corning Incorporated, NY, USA) and maintained in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL of streptomycin. The flasks were kept in a humidified incubator at 37°C under 5.0% CO2 in air.
The 140 mM isotonic NaCl solution (NaCl buffer) contained 140 mM NaCl, 5.0 mM KCl, 1.0 mM CaCl2, 1.0 mM MgCl2, 5.0 mM glucose, and 10 mM HEPES.
The 140 mM isotonic NaCl solution (NaCl buffer) contained 140 mM NaCl, 5.0 mM KCl, 1.0 mM CaCl2, 1.0 mM MgCl2, 5.0 mM glucose, and 10 mM HEPES.
5
Characterization of Glioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human malignant glioma cell lines A-172 (cat. no. JCRB0228; lot no. 021999), AM-38 (cat. no. IFO50492; lot no. 12082003), T98G (cat. no. IFO50303; lot no. 1007), U-251MG (cat. no. IFO50288; lot no. 12132002) and YH-13 (cat. no. IFO50493; lot no. 1164) were purchased from the Health Science Research Resources Bank of Japan. U-138MG (cat. no. HTB-16; lot no. 1104428) was purchased from the American Type Culture Collection. A previous study by our group confirmed that O6-methylguanine-DNA methyltransferase (MGMT), a key factor of alkylating agents, was expressed in the T98G, U-138MG and YH-13 cell lines via RT-qPCR and western blot analyses (22 (link)). Consistent with an earlier study (23 (link)), it was also confirmed that the T98G (237 Met→Ile) and U-251MG (273 Arg→His) cell lines have a point mutation in the TP53 gene (data not shown).
Cells were cultured in Dulbecco's modified Eagle's medium (Nissui Pharmaceutical) supplemented with 10% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C incubator in a humidified (>95%) atmosphere containing 5% CO2.
The antiepileptic drugs perampanel (kindly gifted by Eisai Co., Ltd.), CBZ (Tokyo Chemical Industry), VPA (Tokyo Chemical Industry) and LEV (Tokyo Chemical Industry) and the anti-cancer agent TMZ (Tokyo Chemical Industry) were employed for this study.
Cells were cultured in Dulbecco's modified Eagle's medium (Nissui Pharmaceutical) supplemented with 10% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C incubator in a humidified (>95%) atmosphere containing 5% CO2.
The antiepileptic drugs perampanel (kindly gifted by Eisai Co., Ltd.), CBZ (Tokyo Chemical Industry), VPA (Tokyo Chemical Industry) and LEV (Tokyo Chemical Industry) and the anti-cancer agent TMZ (Tokyo Chemical Industry) were employed for this study.
6
Comparative Analysis of Human Glioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human malignant glioma cell lines A-172 (cat. no. JCRB0228; lot no. 021999), AM-38 (cat. no. IFO50492; lot no. 12082003), T98G (cat. no. IFO50303; lot no. 1007), U-251MG (cat. no. IFO50288; lot no. 12132002) and YH-13 (cat. no. IFO50493; lot no. 1164) were purchased from Health Science Research Resources Bank. The human glioblastoma U-138MG cell line (cat. no. HTB-16; lot no. 1104428) was purchased from the American Type Culture Collection.
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM) (Nissui Pharmaceutical, Co., Ltd.) supplemented with 5% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C humidified incubator with an atmosphere containing 5% CO2.
Perampanel was gifted by Eisai Co., Ltd. and TMZ was purchased from Tokyo Chemical Industry Co., Ltd.
Cells were cultured in Dulbecco's modified Eagle's minimum essential medium (DMEM) (Nissui Pharmaceutical, Co., Ltd.) supplemented with 5% fetal calf serum (Thermo Fisher Scientific, Inc.) using plastic culture flasks (Corning, Inc.) in a 37°C humidified incubator with an atmosphere containing 5% CO2.
Perampanel was gifted by Eisai Co., Ltd. and TMZ was purchased from Tokyo Chemical Industry Co., Ltd.
7
Culturing Human Liver Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines, HLE and Alexander, were obtained from the Japanese Collection of Research Bioresources Cell Bank. These cells, which had undergone less than thirty passages, were used in all analyses. They were grown in plastic culture flasks (Corning Incorporated, NY, USA); HLE cells were maintained in DMEM medium (Nacalai Tesque, Kyoto, Japan) and Alexander cells were maintained in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan). Each medium was supplemented with 10% fetal bovine serum (FBS), 100 U/ml of penicillin, and 100 μg/ml of streptomycin. Flasks were kept in a humidified incubator at 37ºC under 5.0% CO2 in air. NPPB was purchased from BIOMOL International, L.P. (Plymouth Meeting, PA, USA). Quin and Hg were purchased from Nacalai Tesque.
8
Culturing Microbial Pathogens and Fish Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fish pathogen E. piscicida (formerly known as E. tarda) has been reported previously [35 (link)]. Vibrio anguillarum and fish infectious spleen and kidney necrosis virus (ISKNV) were kindly provided by Dr. Min Zhang of Qingdao Agricultural University, and viral proliferation was reported previously [36 (link)]. Escherichia coli was purchased from Transgene (Beijing, China). Bacterial strains were cultured in Luria–Bertani broth (LB) medium at 37 °C (for E. coli) or at 28 °C (all other microbes).
The FG cell line FG-9307 was derived from the gills of Japanese flounder and maintained according to the method described by Tong [37 (link)]. Briefly, FG cells were cultured in Leibovitz’s L-15 with L-glutamine (L-15; Solarbio Science & Technology Co., Ltd., Beijing, China) supplemented with 10% bovine calf serum (BCS; HyClone, Utah, USA), 100 IU mL penicillin and 100 mg/mL streptomycin in plastic culture flasks (Corning, New York, USA) at 23 °C.
The FG cell line FG-9307 was derived from the gills of Japanese flounder and maintained according to the method described by Tong [37 (link)]. Briefly, FG cells were cultured in Leibovitz’s L-15 with L-glutamine (L-15; Solarbio Science & Technology Co., Ltd., Beijing, China) supplemented with 10% bovine calf serum (BCS; HyClone, Utah, USA), 100 IU mL penicillin and 100 mg/mL streptomycin in plastic culture flasks (Corning, New York, USA) at 23 °C.
9
Characterization of Human Glioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human malignant glioma cells of the A-172 (cell no. JCRB0228, lot no. 021999), AM-38 (cell no. IFO50492, lot no. 12082003), T98G (cell no. IFO50303, lot no. 1007), U-251MG (cell no. IFO50288, lot no. 12132002), and YH-13 (cell no. IFO50493, lot no. 1164) cell lines were obtained from Health Science Research Resources Bank (Osaka, Japan), and U-87MG (glioblastoma of unknown origin; catalog no.: HTB-14, lot no. 2497162) and U-138MG (catalog no. HTB-16, lot no. 1104428) were procured from the American Type Culture Collection. It has been confirmed by us that O6-methylguanine-DNA methyltransferase (MGMT: a key factor of alkylating agents) is expressed in T98G, U-138MG and YH-13 by RT-PCR and western blot analysis in a previous study (22 (link)). Consistent with an earlier report (23 (link)), it was also confirmed that T98G (237 Met→Ile) and U-251MG (273 Arg→His) have a point mutation of the p53 gene. These cell lines were cultured in Dulbecco's modified Eagle's medium (Nissui Pharmaceutical) containing 10% fetal calf serum (Life Technologies) using plastic culture flasks (Corning®) in a standard humidified incubator at 37°C under a 5% CO2, 95% air atmosphere.
10
Evaluating Melanoma Cell Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Natural-type of IFN-β (Toray Industries, Tokyo, Japan), TMZ (Tokyo Chemical Industry, Tokyo, Japan) and O 6 -benzylguanine (O 6 -BG; Abcam, Cambridge, UK) were used for the experiments.
Cell lines. Human malignant melanoma A375, CRL-1579, G361, MeWo and SK-MEL-28 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were routinely cultured in Dulbecco's modified Eagle's medium (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA) using plastic culture flasks (Corning Inc., Corning, NY, USA) in a humidified incubator at 37˚C with an atmosphere containing 5% CO 2 .
Cell lines. Human malignant melanoma A375, CRL-1579, G361, MeWo and SK-MEL-28 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were routinely cultured in Dulbecco's modified Eagle's medium (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA) using plastic culture flasks (Corning Inc., Corning, NY, USA) in a humidified incubator at 37˚C with an atmosphere containing 5% CO 2 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!