Anti β cop

Manufactured by Merck Group

Anti–β-COP is a laboratory reagent used for the detection and purification of the beta subunit of the coatomer protein complex I (COPI). It functions as a specific antibody that binds to the beta-COP protein, allowing its identification and isolation from biological samples.

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Lab products found in correlation

Anti tubulin, by Merck Group (2 mentions) Goat anti rabbit dylight 488, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg, by Merck Group (1 mentions) Mouse anti ha tag, by Merck Group (1 mentions) Rabbit anti gm130, by Abcam (1 mentions) Rabbit anti calnexin, by Merck Group (1 mentions) Goat anti rabbit igg hrp, by Jackson ImmunoResearch (1 mentions) Goat anti mouse immunoglobulin g igg, by Jackson ImmunoResearch (1 mentions) Goat anti mouse dylight 594, by Thermo Fisher Scientific (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti gm130, by BD (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Anti gbf1, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-β-COP antibody (1 citations)

2 protocols using anti β cop

Comprehensive Antibody Characterization Protocol

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Mouse anti-HA tag, anti–β-COP, and anti-tubulin were from Sigma-Aldrich, and anti-GFP was from Santa Cruz Biotechnology (Dallas, TX). Rabbit anti–GM-130 was from Abcam (Cambridge, England). Mouse anti-human ERManI was a kind gift from Richard Sifers (Baylor College, Houston, TX) (used only for human cells) or was from Santa Cruz Biotechnology (used for mouse cells). Rabbit anti-calnexin was from Sigma-Aldrich, and anti-dsRED was from MBL. Goat anti-mouse immunoglobulin G (IgG) linked to horseradish peroxidase (HRP) and goat anti-rabbit IgG-HRP were from Jackson Labs (West Grove, PA). Goat anti-mouse Dylight 594 and goat anti-rabbit Dylight 488 were from Thermo Scientific (Barrington, IL). Rabbit polyclonal anti-H2 N-terminus was previously described (Tolchinsky et al., 1996 (link)), Goat anti-mouse IgG linked to agarose beads was from Sigma-Aldrich. Rabbit anti-Cab45 was described before (Scherer et al., 1996 (link)).

Benyair R., Ogen-Shtern N., Mazkereth N., Shai B., Ehrlich M, & Lederkremer G.Z. (2015). Mammalian ER mannosidase I resides in quality control vesicles, where it encounters its glycoprotein substrates. Molecular Biology of the Cell, 26(2), 172-184.

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Immunofluorescence Staining of Lipid Droplet Proteins

Cited 1 time

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Rabbit polyclonal antibodies used: anti-GPAT4 (Wilfling et al., 2013 (link)), anti-CCT1 (Wilfling et al., 2013 (link)), anti-GBF1 (BD Biosciences, San Jose, CA), anti-KDEL-receptor (KDELR; gift from Dr JE Rothman; Yale University), anti-βCOP (gift from Dr JE Rothman; Yale University), anti-perilipin3 (TIP47; Novus Biologicals, Littleton, CO), anti-αCOP (Abcam, Cambridge, MA), anti-GRP78/BiP (ET-21) (Sigma–Aldrich, St. Louis, MO) and anti-garz (Wang et al., 2012 (link)) (gift from Dr A Paululat; University of Osnabrück). Mouse monoclonal antibodies used: anti-GM130 (BD Biosciences), anti-tubulin (Sigma–Aldrich), anti-β’COP (gift from Dr JE Rothman; Yale University), and anti-clathrin heavy chain (x22) (Thermo Scientific, Waltham, MA) antibody. The following secondary antibodies were used: Alexa Fluor 568 goat anti-rabbit (Invitrogen, Grand Island, NY), Alexa Fluor 488 goat anti-mouse (Invitrogen), ATTO 647N (STED) goat anti-rabbit (Active Motif, Carlsbad, CA), and goat anti-mouse STAR470SX (Abberior, Göttingen, Germany).

Wilfling F., Thiam A.R., Olarte M.J., Wang J., Beck R., Gould T.J., Allgeyer E.S., Pincet F., Bewersdorf J., Farese RV J.r, & Walther T.C. (2014). Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting. eLife, 3, e01607.

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