HA1800 and U87 MG cells were preserved and passaged in our laboratory. These cells were grown in DMEM (Meilunbio, China) with 10% fetal bovine serum (FBS) (Meilunbio, China), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Meilunbio, China). Cells were cultured in a humidified atmosphere of 5% CO2 at 37°C.
Penicillin
Manufactured by Meilun
Sourced in China
Penicillin is a laboratory equipment used for the production and purification of the antibiotic compound penicillin. It is a vital tool in the pharmaceutical industry and research laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Meilun (10 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Meilun (3 mentions)
Dulbecco modified eagle medium (dmem), by Meilun (3 mentions)
Dulbecco s modified eagle s medium dmem, by Meilun (2 mentions)
Rpmi 1640 medium, by Meilun (1 mentions)
Mda mb 231, by Meilun (1 mentions)
3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (1 mentions)
Rosiglitazone, by Selleck Chemicals (1 mentions)
Insulin, by Solarbio (1 mentions)
Dmem f12, by Meilun (1 mentions)
Fetal bovine serum (fbs), by Solarbio (1 mentions)
Snb19, by BNCC (1 mentions)
Hs683, by BNCC (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Heparin, by Meilun (1 mentions)
Bicinchoninic acid bca protein assay kit, by Meilun (1 mentions)
12 protocols using penicillin
1
Cell Culture of HA1800 and U87 MG
2
Culturing Murine Cancer Cell Lines
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Murine B16-F10 melanoma and T241 fibrosarcoma cell lines were kindly provided by Dr. Yihai Cao from the Karolinska Institute. B16-F10, T241, and primary megakaryocytes were cultured in 10% FBS-DMEM (catalog no. 40130ES76, YEASEN; catalog no. TBD10569, TBD Science) containing 100 U/mL penicillin, 100 mg/mL streptomycin (catalog no. MA0110, Meilunbio). Isolated platelets were cultured in PBS with 0.25 mmol/L prostaglandin E1 (catalog no. MA1009, Meilunbio). All cell lines used in our study were regularly tested for Mycoplasma (catalog no. LT07-318; Lonsa).
3
Culturing Human Breast Cancer Cell Lines
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The MCF10A (cat. no. CL-0525) cell line was purchased from Wuhan Procell Company, derived from human normal mammary epithelial cells, and was cultured in specialized MCF10A medium (Procell Life Science & Technology Co., Ltd.). The human BC cell lines, SKBR3 (cat. no. 1101HUM-PUMC000085), MCF7 (cat. no. 1101HUM-PUMC000013) and Hs578T (cat. no. 1101HUM-PUMC000670), were cultured in DMEM (Dalian Meilun Biology Technology Co.) supplemented with 10% fetal bovine serum (Shanghai ExCell Biological Products Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (Dalian Meilun Biology Technology Co., Ltd.) in a 5% CO2 incubator at 37°C, while the MDA-MB-231 (cat. no. 1101HUM-PUMC000014) and MDA-MB-468 cells (cat. no. 1101HUM-PUMC000249) were cultured in RPMI-1640 medium (Dalian Meilun Biology Technology Co.). All the human BC cell lines were purchased from the ChineseNational Infrastructure of Cell line Resource (NICR).
4
Culturing Gastric and Epithelial Cells
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Gastric cancer cell line MGC 803 and mucosal epithelial cell line GES-1 were obtained from Cell Bank, Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). The cells were cultured in RPMI 1640 medium (Meilunbio, Cat. No.: MA 0215) supplemented with 10% fetal bovine serum (FBS, Gibco, Cat. No.: 10099–141), and 1% penicillin and streptomycin (Meilunbio, Cat. No.:MA0110) in a humidified incubator with 5% CO2 at 37°C.
5
Isolation and Culture of Goat Brown and White Adipocytes
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The brown preadipocytes were isolated from goat perirenal BAT at 1 day after birth. After washing with phosphate-buffered saline (PBS), the BAT was digested with type Ⅱ-collagen enzyme (2 mg/mL) (BioFrox, Guangzhou, China) for 25 min at 37 °C, followed by filtration through a 70 µm filter. After centrifugation at 1500× g for 5 min, we added the medium containing 10% FBS (Gibco, Emeryville, CA, USA), streptomycin, penicillin, and DMEM/F12 (Meilunbio, Dalian, China), and transferred it to the culture bottle under conditions of 5% CO2 and 37 °C.
Primary white adipocytes were obtained from the perirenal WAT at 30 days after birth. Brown and white adipocytes were cultured in media supplemented with 10% FBS, 860 nM insulin (Solarbio, Beijing, China), 1 nM triiodothyronine(T3) (Selleck, Houston, TX, USA), 1 μM rosiglitazone (Selleck, TX, USA), 1 µM dexamethasone (MCE, Shanghai, China), and 0.5 mM IBMX (Sigma, Marlborough, MA, USA) for 2 days. Cells were then treated in maintenance media (2% FBS, 860 nM insulin, and 1 nM T3) for 6 days.
Primary white adipocytes were obtained from the perirenal WAT at 30 days after birth. Brown and white adipocytes were cultured in media supplemented with 10% FBS, 860 nM insulin (Solarbio, Beijing, China), 1 nM triiodothyronine(T3) (Selleck, Houston, TX, USA), 1 μM rosiglitazone (Selleck, TX, USA), 1 µM dexamethasone (MCE, Shanghai, China), and 0.5 mM IBMX (Sigma, Marlborough, MA, USA) for 2 days. Cells were then treated in maintenance media (2% FBS, 860 nM insulin, and 1 nM T3) for 6 days.
6
Cell Culture Protocol for Glioma Lines
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Glioma cell lines (HS683, U251, T98G, and SNB-19) and human normal astrocyte (HEB) were purchased from BeNa Culture Collection (Beijing, China), and cultured in DMEM (BI, Israel) supplemented with 10% FBS (Solarbio, Beijing, China), and 100 U/mL penicillin and 100 mg/mL streptomycin (Meilunbio, Dalian, China).
7
Culturing Brain Capillary Endothelial Cells and Neuroblastoma Cells
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Brain capillary endothelial cells (BCECs), sharing common characteristic with the BBB, were cultured in Dulbecco's modified Eagle's medium (DMEM, Thermo Fisher Scientific) containing 20% heat-inactivated fetal bovine serum (FBS, Gibco, Waltham, MA, USA), 100 mg/L epidermal cell growth supplement (Thermo Fisher Scientific), 300 mg/L l -glutamine, 20 mg/L heparin, 100 U/mL penicillin and 100 mg/L streptomycin (Meilunbio, Dalian, China). Specially, all the culture dishes or inserts for BCECs were coated with gelatin (2% in H2O) before usage. SH-SY5Y cells (human neuroblastoma) were grown in DMEM: F-12 (DMEM/F-12, Thermo Fisher Scientific) containing 10% FBS, 100 U/mL penicillin and 100 mg/L streptomycin. All cells were incubated at 37 °C under the condition of 5% CO2 and saturating humidity.
8
Nanohydroxyapatite Cytotoxicity and Immunomodulation
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The nHA (purity >97%, CAS 1306-06-5) was purchased from Aladdin Industrial Inc. (Shanghai, China). Cell Counting Kit-8 (CCK-8), Annexin V-FITC/PI apoptosis detection kit, bicinchoninic acid (BCA) protein assay kit, Dulbecco’s modified Eagle’s medium (DMEM), trypsin, fetal bovine serum (FBS), penicillin and streptomycin were supplied by Meilunbio (Dalian, China). EasySep™ mouse T cell isolation kit was obtained from StemCell Technologies (Vancouver, CA, United States). Anti-mouse CD3 (Clone 17A2), anti-mouse CD28 (Clone 37.51) were purchased from Peprotech (Westlake Village, CA, United States). Anti-mouse CD4, phycoerythrin (PE, Clone GK1.5), Anti-mouse CD8α, phycoerythrin-cyanine 7 (PE-Cy7, Clone 53-6.7) and flow cytometry staining buffer was purchased from Multi Sciences Biotech, Co., Ltd. (Hangzhou, China). 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester (CFSE) was obtained from Abbkine Bioadvisers. Alizarin red S (ARS) staining kit for osteogenesis was purchased from Beyotime (Shanghai, China). Dimethyl sulfoxide (DMSO) and RPMI 1640 culture medium were obtained from Solarbio (Beijing, China). Deionized (DI) water was used in all experiments. All chemicals were used without additional purification.
9
Fabrication and Evaluation of CTAC-based Nanoparticles
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Hexadecyl trimethyl ammonium chloride (CTAC, >99%) was obtained from Sinopharm Group Chemical Reagent Co., LTD (Shenyang, China). Triethanolamine (TEA, >95%) was purchased from Tianjin Bodi Chemical Co., Ltd (Tianjin, China). Tetraethoxysilane (TEOS, 98%) was purchased from Sigma-Aldrich (Shanghai, China). ICG, 1,3-diphenyl isobenzofuran (DPBF), membrane protein extraction kit, Annexin V-FITC apoptosis detection kit, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT), cholecystokinin octapeptide (CCK-8) assay kit, Dulbecco's Modified Eagle's Medium (DMEM), RPMI 1640, PBS (pH 7.4), fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin were all purchased from Meilun Biotechnology Co., Ltd (Dalian, China). Nitric oxide (NO) assay was acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China). The mouse interleukin 12 (IL-12), mouse tumor necrosis factor-alpha (TNF-α) uncoated enzyme-linked immunosorbent assay (ELISA) kits, FITC-labeled F4/80, PE-labeled CD206 and APC-labeled CD86 monoclonal antibody were purchased from Thermo Fisher Scientific.
10
Nanoparticle-Mediated Anti-Inflammatory Assays
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NAR was obtained from Wuhan Yuancheng Gongchuang Technology Co., Ltd. (Wuhan, China). Minimum Eagle's medium/Earle's balanced salt solution (MEM/EBSS) was purchased from Hyclone Laboratories, Inc. (Utah, USA). Lutrol® poloxamer 407 (F127) and Cremophor® EL were supplied by BASF Co., Ltd. (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH–DA), phosphate-buffered saline (PBS), penicillin, and streptomycin sulfate were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Dulbecco's modified Eagle's medium (DMEM), nonessential amino acid, sodium pyruvate, and fetal bovine serum (FBS) were provided by Gibco (Carlsbad, CA). BCA assay kits were supplied by Shenyang Dingguo Biotechnology Co., Ltd. (Shenyang, China). β-glucuronidase and SiO2 were purchased from Sigma-Aldrich (Missouri, USA). The Caco-2 cell line and RAW 264.7 cell line were supplied by the American Type Culture Collection (Manassas, USA). Bovine Type II Collagen and incomplete Freund's adjuvant were purchased from Chondrex (Washington DC, USA). Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were purchased from ABclonal Technology Co., Ltd. (Wuhan, China). All other reagents were of analytical grade.
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