The human colon adenocarcinoma Caco-2 cell line was purchased from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM medium (#11995065, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (#10270106, Gibco), 100 µg/mL streptomycin and 100 U/mL penicillin (#10378016, Gibco) in a humidified 5% CO2 atmosphere at 37 °C. LPS (#L6529, Sigma-Aldrich, St. Louis, MO, USA) or S100A9 (#ab95909, Abcam, Cambridge, MA, USA) was dissolved in PBS. Cells were pretreated with 0.1, 1, 10 µM HSC for 1 h, and then 1 µg/mL LPS or recombinant S100A9 protein was added into the cells. Samples were collected at the corresponding time.
Caco 2 cell line
Manufactured by Shanghai Cell Bank
Sourced in China
The Caco-2 cell line is a widely used model for studying intestinal permeability and drug absorption. Caco-2 cells are derived from a human colorectal adenocarcinoma and spontaneously differentiate into a monolayer of polarized intestinal epithelial cells when cultured. This cell line exhibits morphological and functional characteristics of the human intestinal epithelium, making it a valuable tool for evaluating the absorption and transport of drugs and other compounds.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
Cytochalasin d, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
Nystatin, by Merck Group (1 mentions)
High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
5 n ethyl n isopropyl amiloride eipa, by Merck Group (1 mentions)
Alkaline phosphatase alp assay kit, by Beyotime (1 mentions)
Bile salt, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
3 protocols using caco 2 cell line
1
Caco-2 Cell Culture and Inflammatory Stimuli
2
Characterization and Functional Assessment of Angelica Polysaccharide
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Angelica sinensis polysaccharide was obtained and characterized according to our previous studies. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), hydroxysuccinimide (NHS), succinic anhydride (SA), and Cy5.5 azide (Cy5.5-N3) were purchased from Lumiprobe Corporation (Hallandale Beach, FL). Sodium azide, nystatin, chlorpromazine, cytochalasin D, and 5-(N-ethyl-N-isopropyl) amiloride (EIPA) were obtained from Sigma-Aldrich (St. Louis, MO). Caco-2 cell line was purchased from the Shanghai Cell Bank of Chinese Academy of Sciences. Dulbecco’s modified Eagle’s medium (DMEM, high-glucose), fetal bovine serum (FBS), nonessential amino acids, penicillin, streptomycin, and 0.25% trypsin-EDTA solution were obtained from Gibco BRL (Gaithersburg, MD). All other chemical reagents were analytical reagent grade.
3
Soy Isoflavones Bioavailability Assay
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Soy isoflavones (SIF) sample with a purity of 80% was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Bile salts, pepsin (3000 U/mg), and pancreatin (200 U/mg) were sourced from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM) was obtained from Gibco (Shanghai, China). The Alkaline Phosphatase (ALP) Assay Kit was acquired from the Beyotime Institute of Biotechnology (Beijing, China). The Caco-2 cell line was provided by the Shanghai Cell Bank (Shanghai, China). All other chemicals used were of analytical grade.
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