For the BCAR4 luciferase reporter assay, pRL-TK-BCAR4 or pRL-TK-BCAR4 mutant vectors and pGL3 control (Promega, Madison, WI) were transfected into HEK-293T cells along with miR-644a mimic or miR-644a inhibitor using ExFect® Transfection reagent (Vazyme) according to the instructions of the manufacturer. For miR-644a target gene CCR7 luciferase reporter assay, pRL-TK-CCR7-3΄UTR or pRL-TK- CCR7-3΄UTR mutant vectors and pGL3 control (Promega) were transfected into HEK-293T cells along with miR-644a mimic or miR-644a inhibitor using ExFect® Transfection reagent (Vazyme). The luciferase activity was measured 48 h after transfection using the Dual-Glo luciferase reporter assay system (Promega) according to the guidelines of the manufacturer. Luminescence values were recorded by a Victor X2 multilabel reader (PerkinElmer).
Prl tk bcar4
Manufactured by Promega
The PRL-TK-BCAR4 is a laboratory equipment product offered by Promega. It is a tool designed for specialized applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation. Further information may be available from Promega directly.
Automatically generated - may contain errors
1 protocol using prl tk bcar4
1
Luciferase Assays for miR-644a Targets
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