2 6 diisopropylphenol

Manufactured by Merck Group

Sourced in United States

2,6-diisopropylphenol is a lab equipment product. It is a chemical compound with the formula (CH3)2CHCH2C6H3(OH)(CH(CH3)2). This product serves a core function in laboratory settings, but a detailed description while maintaining an unbiased and factual approach cannot be provided.

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Propofol (2,6-diisopropylphenol) (7 citations)

5 protocols using 2 6 diisopropylphenol

Propofol Effects on Fly Behavior

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A 100 millimolar (mM) stock solution of propofol was prepared by dissolving 9 microliters (μL) of propofol (2,6-Diisopropylphenol, >97%, Sigma-Aldrich, St. Louis MO) in 491 uL of polyethylene glycol (PEG400). Activity tubes containing various concentrations of propofol were prepared by diluting an appropriate volume of 100 mM propofol stock solution in regular fly food (5% sucrose, 2% agar).
Propofol was administered by individually transferring flies to activity tubes with sucrose food containing various doses of propofol or equivalent amounts of vehicle. Activity data was collected for three baseline days during photoentrainment prior to administering propofol treatment on the fourth day for 6h from ZT 0 to ZT 6 (ZT = zeitgeber time, where ZT 0–12 = lights on, and ZT 12–24 = lights off), or as otherwise indicated. Post-propofol treatment, flies were transferred back to freshly prepared activity tubes containing regular sucrose medium, and behavioral data was collected for an additional 3–7 days after drug exposure.

Gardner B., Strus E., Meng Q.C., Coradetti T., Naidoo N.N., Kelz M.B, & Williams J.A. (2016). Sleep Homeostasis and General Anesthesia: Are Fruit Flies Well-Rested After Emergence From Propofol?. Anesthesiology, 124(2), 404-416.

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Synthesis and Characterization of Polyaniline-based Composites

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Polyaniline-dinonylnaphthalene sulfonic acid (PAC 1003, Crosslink, commercial grade is available from Boron Molecular, Noble Park, Victoria, Australia), hydroquinone (HQ, Sigma-Aldrich, St. Louis, MO, USA), thymol (Sigma-Aldrich), carvacrol (Sigma-Aldrich), N-ethyl-toluenesulfonamide (E-TSAm, Sigma-Aldrich), N-hydroxy-benzenesulfonamide (HBSAm, Fluka, Buchs, Switzerland), 4,4′-biphenol (BP, Sigma-Aldrich), 4,4′-sulfonyldiphenol (SDP, Acros, Hampton, NH, USA), hydroxybenzenesulfonic acid (HBSA, Sigma-Aldrich), p-toluenesulfonic acid (PTSA, J.T. Baker, Phillipsburg, NJ, USA), p-toluenesulfonamide (TSAm, Acros), 2-isopropylphenol (IPA, Sigma-Aldrich), 2,6-diisopropylphenol (Sigma-Aldrich), sulfuric acid (Sigma-Aldrich), acetone (Sigma-Aldrich), butyl cellosolve (Sigma-Aldrich), and xylene (Sigma-Aldrich) were a reagent grade and were used as received.

Kim Y.G., Nguyen H.L, & Kinlen P. (2021). Secondary Dopants of Electrically Conducting Polyanilines. Polymers, 13(17), 2904.

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Anesthesia Administration and Euthanasia in Mice

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All mice were housed at room temperature on a 12‐h light–dark cycle for at least 1 week prior to experimentation, and food and water were given ad libitum. Anesthesia was induced and maintained based on previous studies and titration experiments 19, 20. Specifically, anesthesia was induced with 5% Sevo and maintained with 3% Sevo for 3 h. For anesthesia using Prop, a working stock of 25 mg·mL⁻¹ 2,6‐diisopropylphenol (Sigma‐Aldrich, St. Louis, MO, USA) diluted in intralipid (20% emulsion available at Sigma‐Aldrich) was prepared. Mice were intraperitoneally administered 600 mg·kg⁻¹ Prop. Animals under anesthesia were kept on a heating element at 37 °C. The effect of anesthesia was confirmed by examining the pedal reflex every 5 min. Mice were kept under anesthesia using each type of anesthetic for ~ 3 h. For the analysis, mice were euthanized by CO₂ inhalation.

Oda‐Kawashima K., Sedukhina A.S., Okamoto N., lytvyn M., Minagawa K., Iwata T., Kumai T., Sato E., Inada E., Yamaura A., Sakamoto M., Roche‐Molina M., Bernal J.A, & Sato K. (2020). NF‐kB signaling in cardiomyocytes is inhibited by sevoflurane and promoted by propofol. FEBS Open Bio, 10(2), 259-267.

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In Vitro Anesthesia Exposure Protocol

Cited 1 time

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Coverslips in 12-well plates were placed in identical air-tight, humidified chambers (Billups-Rothenberg, Del Mar, CA, USA) as previously described [43 (link)]. Isoflurane (Baxter Healthcare Cooperation, Deerfield, IL, USA) or sevoflurane (AbbVie Inc., North Chicago, IL, USA) were delivered using an agent-specific, calibrated inline vaporizer (SuperaVet, Vaporizer Sales and Services Inc., Rockmart, GA, USA), and were diluted in 5% CO₂/95% O₂ carrier gas. Controls for these experiments received 5% CO₂/95% O₂ carrier gas only. There was a 15-min equilibration period, which was required to achieve the correct concentration of Isoflurane or sevoflurane as measured by a 5250 RGM gas analyzer (Datex-Ohmeda, Madison, WI, USA). Then the sealed chambers were placed in an incubator to maintain temperature at 37 °C for the duration of anesthesia exposure. Isoflurane/sevoflurane concentration was periodically measured at the end of the experimental period to verify that it was appropriately maintained throughout the exposure.
The propofol exposure was done by adding pure 2,6-diisopropylphenol (Sigma Aldrich, Saint Louis, MO, USA) (1 nM, 2 nM, 4 nM) into experiment wells, and incubated at 37 °C for the duration of anesthesia exposure. The exposure was terminated by removing all the media and by adding a combination of previously-removed media without propofol and fresh media.

Xu J., Mathena R.P., Xu M., Wang Y., Chang C., Fang Y., Zhang P, & Mintz C.D. (2018). Early Developmental Exposure to General Anesthetic Agents in Primary Neuron Culture Disrupts Synapse Formation via Actions on the mTOR Pathway. International Journal of Molecular Sciences, 19(8), 2183.

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Synthesis of Propofol-Fatty Acid Conjugates

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The raw materials needed for the synthesis of propofolfatty acid ester conjugates of selected four fatty acids, such as alpha-linolenic acid (ALA) , was extracted from eripupal oil according to a reported procedure 41) , valproic acid (VA) was purchased from Alfa-Aesar Chemicals (USA) . Butyric acid (BA) , 2-ethylhexanoic acid (2-EHA) , 2,4-diisopropylphenol, 2,6-diisopropylphenol and N,N -dicyclohexylcarbodiimide (DCC) , 4-(dimethyl amino) pyridine (DMAP) were purchased from Sigma-Aldrich Chemicals (USA) . All solvents and chemicals were of reagent grade and used directly without further purification. Silica gel (60-120 mesh) for column chromatography was purchased from Acme Synthetic Chemicals, Mumbai, India. Precoated TLC plates were purchased from Merck, Darmstadt, Germany. Human tumor cell lines were obtained from American Type Culture Collection, Manassas, VA, USA.

Reddy Y.S., Kaki S.S., Rao B.B., Jain N, & Vijayalakshmi P. (2016). Study on Synthesis, Characterization and Antiproliferative Activity of Novel Diisopropylphenyl Esters of Selected Fatty Acids. Journal of oleo science, 65(1).

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