Anti mouse cd19 apc

Manufactured by Thermo Fisher Scientific

The Anti-mouse CD19 APC is a laboratory reagent used for the identification and analysis of mouse B lymphocytes. It is a fluorescently labeled antibody that binds to the CD19 surface antigen expressed on mouse B cells. This product can be used in flow cytometry applications to detect and quantify CD19-positive cells.

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Lab products found in correlation

Anti mouse cd11c apc, by Thermo Fisher Scientific (1 mentions) Anti mouse ifn γ apc, by Thermo Fisher Scientific (1 mentions) Mouse igg1 κ isotype control, by Thermo Fisher Scientific (1 mentions) Easysep mouse cd8 t cell isolation kit, by STEMCELL (1 mentions) Rat igg1 κ isotype control ebrg1, by Thermo Fisher Scientific (1 mentions) Easysep mouse nk cell isolation kit, by STEMCELL (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)

2 protocols using anti mouse cd19 apc

Comprehensive Immune Cell Profiling

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Anti-human CD45 FITC (HI30), anti-human TIGIT APC (MBSA43), anti-human PD-1 PE (MIH4), anti-mouse TIGIT PE (GIGD7), anti-mouse PVR APC (TX56), anti-mouse PD-1 PE (J43), anti-mouse PD-L1 PE (MIH5), anti-mouse CD45 FITC (30-F11), anti-mouse CD3 PerCP-eFluor710 (17A2), anti-mouse CD8α PE (53-6.7) anti-mouse CD49b PE/APC (DX5), anti-mouse CD19 APC (eBio1D3), anti-mouse CD11c APC (N418), anti-mouse CD11b APC (M1/70), anti-mouse Ly-6G(Gr-1) PE- Cyanine7 (RB6-8C5), anti-mouse F4/80 PerCP-Cyanine5.5 (BM8), anti-mouse IFN-γ APC (XMG1.2), mouse IgG1κ isotype control (P3.6.2.8.1), rat IgM isotype control (eBR2M), rat IgG2α κ isotype control (eBR2a) and rat IgG1 κ isotype control (eBRG1) antibodies were purchased from eBioscience. Anti-mouse TIGIT APC (1G9) was purchased from BioLegend. Antibodies anti-asialo-GM1 (catalog 986-10001) (Wako Chemicals GmbH, Germany) and rabbit IgG control (I8140) (Sigma) were used for NK cell depletion. EasySep mouse CD8⁺ T cell isolation kit (catalog 19853) and EasySep mouse NK cell isolation kit (catalog 19855) (STEMCELL) were used for cell sorting.

Zhou X.M., Li W.Q., Wu Y.H., Han L., Cao X.G., Yang X.M., Wang H.F., Zhao W.S., Zhai W.J., Qi Y.M, & Gao Y.F. (2018). Intrinsic Expression of Immune Checkpoint Molecule TIGIT Could Help Tumor Growth in vivo by Suppressing the Function of NK and CD8+ T Cells. Frontiers in Immunology, 9, 2821.

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Evaluating CD20-targeted Immunotherapy

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Splenocytes from target hCD20Tg (T) and non-target (NT) wild-type (unless otherwise specified) mice were labelled with 5μM and 0.5μM CFSE (Invitrogen), respectively, mixed (1:1), and i.v injected into recipients (5-8 × 10⁶ cells/mouse). Two doses of adjuvant [TLR1/2a Pam3CSK4: 10-100μg, TLR2/4a LPS: 10μg, TLR3a Poly I:C: 100μg, TR7/8a R848: 2.5μg, DMXAA: 400μg, Type I IFN: 10,000 IU] were administered at 24 and 48h i.p, followed by Ritm2a (25-50μg) or isotype control i.v. For BALB/c mice, DMXAA was administered only once (300μg at 24h). Spleen was harvested 16-20h after Ritm2a administration, splenocytes stained with anti-mouse CD19 APC (eBioscience) and assessed for T:NT ratio. IFNAR blocking mAb (Clone MAR1-5A3; Leinco) was given at 500μg i.p as indicated in the figure legend.

Dahal L.N., Dou L., Hussain K., Liu R., Earley A., Cox K.L., Murinello S., Tracy I., Forconi F., Steele A.J., Duriez P.J., Gomez-Nicola D., Teeling J.L., Glennie M.J., Cragg M.S, & Beers S.A. (2017). STING activation reverses lymphoma-mediated resistance to antibody immunotherapy. Cancer research, 77(13), 3619-3631.

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