In order to predict the molecular formulae as well as elemental composition of AFB1 and AFB2 degradation products, samples were further analyzed by electrospray ionization mass spectrometer. MS/MS was performed on was performed on a Thermo Scientific LTQ XL System fitted with electrospray ionization (ESI) source operating in positive ionization mode with optimum conditions set as follows: capillary voltage to 49.0 V, source voltage to 5.0 KV, Tube lens voltage to 110 V, and capillary temperature to 275°C. Sheath and auxiliary gas flow were adjusted to get stable spray, i.e., 3 L min-1 and 0.4 L min-1, respectively. Data was collected in positive mode within the range of 100 to 500 m/z. The final identification of an unknown compound was based on the accurate measurement of mass of parent ions and fragments, as well as other useful MS/MS spectrum information (Wang et al., 2011 (link)). In MS/MS experiments, untreated aflatoxins and water treated toxins were run as a control.
Ltq xl system
Manufactured by Thermo Fisher Scientific
The LTQ XL System is a linear ion trap mass spectrometer designed for high-performance qualitative and quantitative analysis. It offers high sensitivity, mass accuracy, and resolution for a wide range of applications.
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Lab products found in correlation
5 protocols using ltq xl system
1
Mass Spectrometric Identification of Aflatoxin Degradation Products
2
Mass Spectrometry Characterization of Unknown Compounds
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Mass spectrometry/mass spectrometry was carried out using a Thermo Scientific LTQ XL System equipped with electrospray ionization (ESI) source operating in the positive ionization mode with a capillary voltage of 49.0 V, a source voltage of 5.0 KV, a tube lens voltage of 110 V and a capillary temperature of 275 °C. The sheath and auxiliary gas flow were adjusted to 3 and 0.4 L/min, respectively, to get a stable spray. Data were collected in positive mode within the range of 100 to 500 m/z. The identification of unknown compounds was based on the accurate measurement of the mass of parent ions and fragments, as well as other useful MS/MS spectrum information.
3
Mass Spectrometry Protocol for Compound Identification
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Mass spectrometery/ Mass spectrometery was performed on a Thermo Scientific LTQ XL System fitted with electrospray ionization (ESI) source operating in positive ionization mode with optimum conditions set as follows: capillary voltage to 49.0 V, source voltage to 5.0 KV, Tube lens voltage to 110 V, and capillary temperature to 275 °C. Sheath and auxillary gas flow were adjusted to get stable spray i.e., 3 L min−1 and 0.4 L min−1 respectively. Data was collected in positive mode within the range of 100 m/z to 500 m/z. The final identification of an unknown compound was then performed based on the accurate measurement of mass of parent ions and fragments, as well as other useful MS/MS spectrum information.
4
Synthesis of N-myristoyl-D-Asn (NMDA)
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The synthesis of N-myristoyl-D-Asn (NMDA) was performed as previously described [45 (link)], with modifications. All the solvents and reagents were purchased from commercial suppliers and used without further purification. N2-Fmoc-N4-trityl-D-asparagine [Fmoc-D-Asn(Trt)-OH], 2-chlorotrityl chloride resin (1.0mmol/g, 100~200mesh, 1%DVB) and PyBOP were purchased from GL Biochem (Shanghai) Ltd. 1H NMR was recorded on a Bruker 400 MHz spectrometer at 298 K. All chemical shifts were quoted in ppm and coupling constants were measured in Hz. Electrospray ionization mass spectrum (ESI-MS) of NMDA was measured in negative mode on a Thermo LTQ XL system.
5
Mass Spectrometric Analysis of Aflatoxin Degradation
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Samples were further analyzed by mass spectrometer with electrospray ionization (ESI) to predict the molecular formulae as well as elemental composition of degraded products of AFB1 and AFB2. Mass spectrometery/Mass spectrometery was performed on a Thermo Scientific LTQ XL System fitted with electrospray ionization (ESI) source operating in positive ionization mode with optimum conditions set as follows: capillary voltage to 49.0 V, source voltage to 5.0 KV, Tube lens voltage to 110 V, and capillary temperature to 275°C. Sheath and auxiliary gas flow were adjusted to get stable spray i.e., 3 L min−1 and 0.4 L min−1 respectively. Data were collected in positive mode within the range of 100–500 m/z. The final identification of an unknown compound was based on the accurate mass measurement of parent and fragments ions, as well as other useful MS/MS spectrum information (Wang et al., 2011 (link)). Untreated toxins (AFB1 and AFB2) and water treated toxins were run as control in MS/MS experiments.
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