Igm or igg2b

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IgM and IgG2b are antibody classes produced by the human immune system. IgM is the first antibody class produced in response to an infection, while IgG2b is a subclass of the IgG antibody. Both play important roles in the body's immune response, but a detailed description of their core functions would require medical expertise to provide in an unbiased and factual manner while maintaining conciseness.

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IgG2b (18 citations)

2 protocols using igm or igg2b

Germinal Center Formation and Antibody Response to Influenza Virus

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2 d after the administration of DTxn, mice received 20 μl influenza virus suspension (1 × 10⁶ plaque-forming units) by subcutaneous injection into the footpad. 14 days later, popliteal draining lymph nodes were collected, cryopreserved and imaged for analysis of the formation of germinal centers. Additionally, serum was collected for analysis of influenza virus–specific antibodies. For enzyme-linked immunosorbent assay (ELISA) of serum, influenza virus strain PR8 that had undergone ultraviolet irradiation was immobilized on a plate with high binding capacity, followed by the addition of collected serum and probing for specific binding of IgM or IgG2b (Sigma) by standard alkaline-phosphatase development. For experiments involving immunization with TNP-Ficoll, mice were given injection of DTxn and, 2 d later, were given intraperitoneal injection of TNP-Ficoll (250 μg per mouse). IgM antibody titers in the blood were determined at day 8.

Cremasco V., Woodruff M.C., Onder L., Cupovic J., Nieves-Bonilla J.M., Schildberg F.A., Chang J., Cremasco F., Harvey C.J., Wucherpfennig K., Ludewig B., Carroll M.C, & Turley S.J. (2014). B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nature Immunology, 15(10), 973-981.

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Influenza Virus Infection and Antibody Response

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2 days after DTxn administration, mice received 20 μl influenza virus suspension (1 × 10⁶ p.f.u.) subcutaneously into the footpad. 14 days later, popliteal draining lymph nodes were collected, cryopreserved and imaged to determine formation of germinal centers. Additionally, serum was collected to determine the presence of influenza-specific antibodies. ELISA analysis of serum was carried out through immobilization of UV-PR8 on a high binding plate, addition of collected serum, and probing for specific binding of IgM or IgG2b (Sigma) using standard alkaline phosphatase development. For TNP-Ficoll immunization experiments, mice were injected with DTxn and, 2 days later, with TNP-ficoll i.p. (250 μg/mouse). IgM antibody titers were determined at day 8 in the blood.

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