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Rlt tissue lysis buffer

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The RLT tissue lysis buffer is a solution used in the process of lysing or breaking down tissue samples to extract RNA. It is a key component in RNA isolation and purification workflows.

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Lab products found in correlation

Synergy h1, by Synergy Software (2 mentions)

2 protocols using rlt tissue lysis buffer

1

Quantifying cGAMP and IFNβ in Tumor Draining Lymph Nodes

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Subcutaneous B16.F10 tumours of ~100 mm3 were injected with coformulations of cGAMP (10 μg) and cdGMP-Dy547 (0.2 μg). 1 hr after injection, the mice were sacrificed and the inguinal tumour draining lymph node was harvested, placed in RLT tissue lysis buffer (Qiagen), and homogenized using an OctoMACs tissue dissociator. cdGMP-Dy547 was quantified in the resulting lysate on a Synergy H1 plate reader (576/550 Ex/Em). Background fluorescence was removed by subtracting baseline fluorescence values of TDLN lysates from PBS treated tumour bearing mice. For quantification of Ifnb1 expression, subcutaneous B16.F10 tumours were injected with cGAMP formulations (10 μg). TDLNs were harvested 2 hrs after injection. mRNA isolation, cDNA synthesis, and qPCR quantification of Ifnb1 transcription were performed as described above.
Shae D., Becker K.W., Christov P., Yun D.S., Lytton-Jean A.K., Sevimli S., Ascano M., Kelley M., Johnson D.B., Balko J.M, & Wilson J.T. (2019). Endosomolytic Polymersomes Increase the Activity of Cyclic Dinucleotide STING Agonists to Enhance Cancer Immunotherapy. Nature nanotechnology, 14(3), 269-278.
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2

Quantifying cGAMP and IFNβ in Tumor Draining Lymph Nodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous B16.F10 tumours of ~100 mm3 were injected with coformulations of cGAMP (10 μg) and cdGMP-Dy547 (0.2 μg). 1 hr after injection, the mice were sacrificed and the inguinal tumour draining lymph node was harvested, placed in RLT tissue lysis buffer (Qiagen), and homogenized using an OctoMACs tissue dissociator. cdGMP-Dy547 was quantified in the resulting lysate on a Synergy H1 plate reader (576/550 Ex/Em). Background fluorescence was removed by subtracting baseline fluorescence values of TDLN lysates from PBS treated tumour bearing mice. For quantification of Ifnb1 expression, subcutaneous B16.F10 tumours were injected with cGAMP formulations (10 μg). TDLNs were harvested 2 hrs after injection. mRNA isolation, cDNA synthesis, and qPCR quantification of Ifnb1 transcription were performed as described above.
Shae D., Becker K.W., Christov P., Yun D.S., Lytton-Jean A.K., Sevimli S., Ascano M., Kelley M., Johnson D.B., Balko J.M, & Wilson J.T. (2019). Endosomolytic Polymersomes Increase the Activity of Cyclic Dinucleotide STING Agonists to Enhance Cancer Immunotherapy. Nature nanotechnology, 14(3), 269-278.
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