Mouse proinflammatory chemokine panel

Manufactured by BioLegend

Sourced in United States

The Mouse Proinflammatory Chemokine Panel is a multiplex assay that allows for the simultaneous quantification of multiple mouse chemokines in a single sample. This panel measures the levels of specific chemokines involved in the proinflammatory response.

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Lab products found in correlation

Legendplex software, by BioLegend (3 mentions) Legendplex mouse inflammation panel, by BioLegend (3 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Facscanto 2 cell analyzer, by BD (1 mentions) Legendplex data analysis software, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Legendplex mouse th17 panel, by BioLegend (1 mentions) Complete mini protease inhibitor, by Roche (1 mentions) Triton x 100, by Merck Group (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Facsverse, by BD (1 mentions) Legendplex analysis software, by BioLegend (1 mentions) Tissuelyser, by Qiagen (1 mentions) Legendplex kit, by BioLegend (1 mentions) Legendplex, by BioLegend (1 mentions) Legendplex multi analyte flow assay, by BioLegend (1 mentions)

10 protocols using mouse proinflammatory chemokine panel

Profiling Serum Chemokine Levels

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Serum levels of a total of 13 chemokines were measured by bead-based immunoassays (Mouse Proinflammatory Chemokine Panel, Biolegend, San Diego, CA, USA) according to the manufacturer’s protocol.

Su L., Li N., Tang H., Lou Z., Chong X., Zhang C., Su J, & Dong X. (2018). Kupffer cell-derived TNF-α promotes hepatocytes to produce CXCL1 and mobilize neutrophils in response to necrotic cells. Cell Death & Disease, 9(3), 323.

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Colon Inflammation Cytokine Profiling

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Briefly, harvested colon samples were homogenized in T-PER tissue protein extraction lysis buffer (Pierce, Thermo Fisher Scientific, MA) supplemented with protease inhibitor cocktail (Biotool, Houston, TX), and the supernatants were collected. Then, the local levels of inflammatory mediators in CTHs were measured by using LEGENDplex^TM Multiplex Bead Assays (Biolegend, San Diego, CA), of which the Mouse Proinflammatory Chemokine Panel (Cat. No. 740007, 13-plex for CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES, CCL11/Eotaxin, CCL17/TARC, CCL20/ MIP-3α, CCL22/MDC, CXCL1/KC, CXCL5/LIX, CXCL9/MIG, CXCL10/IP-10 and CXCL13/BLC), Mouse T Helper Cytokine Panel (Cat. No. 740005, 13-plex for IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-21, IL-22, IFN-γ and TNF-α) and Mouse Cytokine Panel 2 (Cat. No. 740134, 13-plex for IL-1α, IL-1β, IL-3, IL-7, IL-11, IL-12p40, IL-12p70, IL-23, IL-27, IL-33, IFN-β, GM-CSF and TSLP) were selected. Cytokines/chemokines was profiled on a BD FACSCanto^TM II Cell Analyzer (BD Biosciences, San Jose, CA), and the FCS file generated was analyzed by using Biolegend's LEGENDplex^TM Data Analysis Software according to the manufacturer's instructions.

Ding P., Li L., Li L., Lv X., Zhou D., Wang Q., Chen J., Yang C., Xu E., Dai W., Zhang X., Wang N., Wang Q., Zhang W., Zhang L., Zhou Y., Gu H., Lei Q., Zhou X, & Hu W. (2020). C5aR1 is a master regulator in Colorectal Tumorigenesis via Immune modulation. Theranostics, 10(19), 8619-8632.

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Quantifying Mouse Inflammatory Markers

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Serum cytokines and chemokines were quantified using the LEGENDplex™ mouse inflammation panel and mouse proinflammatory chemokine panel (BioLegend), respectively. All data were collected on an LSRFortessa and analyzed using LEGENDplex™ software (BioLegend).

Li Y., Elmén L., Segota I., Xian Y., Tinoco R., Feng Y., Fujita Y., Segura Muñoz R.R., Schmaltz R., Bradley L.M., Ramer-Tait A., Zarecki R., Long T., Peterson S.N, & Ronai Z.A. (2020). Prebiotic-Induced Anti-tumor Immunity Attenuates Tumor Growth. Cell reports, 30(6), 1753-1766.e6.

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Quantifying Cytokines and Chemokines

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Cytokines and chemokines in the sera of tumor-bearing WT and Rnf5^−/− mice were quantified using the LEGENDplex^TM mouse inflammation panel and mouse proinflammatory chemokine panel (BioLegend), respectively. All data were collected on an LSRFortessa (BD Biosciences) and analyzed using LEGENDplex^TM software (BioLegend).

Li Y., Tinoco R., Elmén L., Segota I., Xian Y., Fujita Y., Sahu A., Zarecki R., Marie K., Feng Y., Khateb A., Frederick D.T., Ashkenazi S.K., Kim H., Perez E.G., Day C.P., Segura Muñoz R.S., Schmaltz R., Yooseph S., Tam M.A., Zhang T., Avitan-Hersh E., Tzur L., Roizman S., Boyango I., Bar-Sela G., Orian A., Kaufman R.J., Bosenberg M., Goding C.R., Baaten B., Levesque M.P., Dummer R., Brown K., Merlino G., Ruppin E., Flaherty K., Ramer-Tait A., Long T., Peterson S.N., Bradley L.M, & Ronai Z.A. (2019). Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5−/− mice. Nature Communications, 10, 1492.

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Multiplex Inflammatory Profiling of Skin Tissue

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Skin was chopped, frozen at −80°C, pulverized with a Tissuelyser II (QIAGEN) and lysed with 1% Triton X‐100 (Sigma) with cOmplete Mini protease inhibitors (Roche). Total protein was quantified by Pierce BCA Protein Assay kit (Thermo Fisher Scientific). Analytes were quantified using LegendPlex mouse Th17 Panel (IFNγ, TNF‐α, IL‐6, IL‐10, IL‐17A, IL‐17F, IL‐21, IL‐22) and mouse proinflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience). S100A8 was measured by ELISA (Bio Techne).

Linley H., Jaigirdar S., Mohamed K., Griffiths C.E, & Saunders A. (2022). Reduced cutaneous CD200:CD200R1 signaling in psoriasis enhances neutrophil recruitment to skin. Immunity, Inflammation and Disease, 10(7), e648.

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Quantifying Lung Chemokine Levels

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To determine the concentration of different chemokines in the lungs, mice were perfused with PBS, lungs were snap-frozen in liquid nitrogen and stored at −80 °C until further use. Lungs were homogenized in 1 ml of MEM supplemented with 5% FCS, penicilin, streptamycin, and glutamin and were lysed two times for 1.5 min at 25 Hz using a tissue lyser (Qiagen). Samples were centrifuged for 5 min at 3.5×g and supernatant was used. To determine the chemokine concentration the mouse pro-inflammatory chemokine panel (Biolegend) was used with v-bottom plate according to manufacturers protocol. Data was analzed with the Legendplex analysis software (Biolegend).

Welten S.P., Oderbolz J., Yilmaz V., Bidgood S.R., Gould V., Mercer J., Spörri R, & Oxenius A. (2021). Influenza- and MCMV-induced memory CD8 T cells control respiratory vaccinia virus infection despite residence in distinct anatomical niches. Mucosal Immunology, 14(3), 728-742.

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Quantifying Proinflammatory Chemokines

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Concentrations of pro-inflammatory chemokines in cell culture supernatant or peritoneal lavage of mice were analyzed using LEGENDplex kit (Mouse Proinflammatory Chemokine Panel, Biolegend, San Diego, CA, USA) according to the manufacturer’s instructions.

Knuth A.K., Huard A., Naeem Z., Rappl P., Bauer R., Mota A.C., Schmid T., Fleming I., Brüne B., Fulda S, & Weigert A. (2021). Apoptotic Cells induce Proliferation of Peritoneal Macrophages. International Journal of Molecular Sciences, 22(5), 2230.

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Cytokine and Chemokine Profiling in Lungs

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The concentrations of various cytokines and chemokines in lung homogenates were determined by LEGENDplex^TM (Mouse Inflammation panel and Mouse Proinflammatory Chemokine Panel, BioLegend) according to the manufacturer’s protocol.

Dibbern J., Eggers L, & Schneider B.E. (2017). Sex differences in the C57BL/6 model of Mycobacterium tuberculosis infection. Scientific Reports, 7, 10957.

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Quantifying Mouse Inflammatory Markers

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Quantification of Proinflammatory Chemokines

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The levels of proinflammatory chemokines in mouse peritoneal lavage fluid were measured by LEGENDplex™ Multi-Analyte Flow Assays (Biolegend) Mouse Proinflammatory Chemokine Panel (13-plex, Cat. No. 740007). Methods were performed as recommended by the manufacturer.

Liu L., Xue Y., Zhu Y., Xuan D., Yang X., Liang M., Wang J., Zhu X., Zhang J, & Zou H. (2016). Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout. Arthritis Research & Therapy, 18, 268.

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