E coli serotype o111 b4

Manufactured by Merck Group

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E. coli serotype O111:B4 is a strain of the bacterium Escherichia coli. It is a common laboratory strain used for various research and testing purposes. The core function of this strain is to serve as a model organism for studying bacterial genetics, physiology, and behavior.

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Lab products found in correlation

Protein g sepharose column, by GE Healthcare (1 mentions) Limulus amebocyte lysate assay, by Associates of Cape Cod (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Merck Group (1 mentions) Escherichia coli lps, by Merck Group (1 mentions)

Spelling variants of this product

E. coli O111:B4 (78 citations) LPS (E. coli serotype O111: B4, (7 citations) LPS) from E. coli serotype O111:B4 (6 citations)

5 protocols using e coli serotype o111 b4

Anti-β2GPI Antibody Induced APS Model

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The polyclonal anti-β2GPI antibodies were purified from sera of New Zealand rabbits immunized with human β2GPI peptide sequence (35GYVSRGGMRKFICPLTG51) according to our previous methods (23 (link)). The purified anti-β2GPI IgG could recognize human and mouse β2GPI as demonstrated by western blotting and ELISA (23 (link)). Our previous study demonstrated that this anti-β2GPI IgG could induce an APS mouse model. The isotype control antibodies (NR-IgG) from sera of normal rabbits were purified by Protein G Sepharose columns (GE Healthcare, Chicago, IL, USA). All the IgG samples and reagents were subjected to Detoxi-Gel™ (Pierce; Thermo Fisher Scientific, Inc., Waltham, MA, USA) to remove endotoxin contamination (<0.03 EU/ml) using the Limulus amebocyte lysate assay (Associates of Cape Cod, Inc., Falmouth, MA, USA).
C3H/HeN and C3H/HeJ mice (n=8 per treatment group) were twice injected intraperitoneally with anti-β2GPI (100 µg) or NR-IgG (100 µg) at 0 and 48 h. Surgical procedures to obtain peritoneal macrophages or aortas were performed at 72 h after the first injection. In addition, other groups of C3H/HeN and C3H/HeJ mice (n=8 in each group) were challenged with LPS (1 µg/g body weight; E. coli serotype O111:B4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) 2 h before surgical procedures as the positive control.

Wang M., Kong X., Xie Y., He C., Wang T, & Zhou H. (2019). Role of TLR-4 in anti-β2-glycoprotein I-induced activation of peritoneal macrophages and vascular endothelial cells in mice. Molecular Medicine Reports, 19(5), 4353-4363.

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Septic Inflammatory Response Induction

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To induce a septic inflammatory response, a continuous infusion of LPS (E. coli Serotype O111:B4; Sigma-Aldrich, Buchs, Switzerland) was administered at a high-dose induction of 150 μg kg⁻¹ h⁻¹ for 1 h, followed by a maintenance dosage of 15 μg kg⁻¹ h⁻¹ throughout the experiment. To prevent the risk of implausible results and lung injury caused by severe hypoxemia or hypercapnia during LPS infusion, an intervention scheme was established. This scheme was based on the ARDS Network PEEP/FiO2 tables, and ventilation parameters were adjusted when the peripheral oxygen saturation dropped below 92% for 5 min.

Rissel R., Moellmann C., Albertsmeier V., Renz M., Ruemmler R., Kamuf J., Hartmann E.K, & Ziebart A. (2023). Clinical dosage of lidocaine does not impact the biomedical outcome of sepsis-induced acute respiratory distress syndrome in a porcine model. PeerJ, 11, e15875.

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Isolation and Stimulation of Peritoneal Macrophages

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Mice were injected with 3% thioglycollate (Difco, St. Louis, USA). Five days later, peritoneal exudate cells were isolated from the peritoneal cavity by washing with ice-cold RPMI (Sigma, Germany) supplemented with 10% FCS (Gibco, Invitrogen Corporation, Germany). Cells were cultured overnight at 37 °C and 5% CO₂ incubator. Adherent monolayer cells were used as peritoneal macrophages and were cultured at 5 × 10⁵ cells/ml in RPMI supplemented with 10% FCS and stimulated for 20 min with either 100 ng/ml LPS (E. coli, serotype O111:B4, Sigma) or M. tuberculosis H37Rv (MOI 2:1).

Dambuza I.M., Keeton R., Hsu N.J., Allie N., Quesniaux V.F., Ryffel B, & Jacobs M. (2016). Persistent p55TNFR expression impairs T cell responses during chronic tuberculosis and promotes reactivation. Scientific Reports, 6, 39499.

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LPS-induced inflammatory response in cell lines

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U-2 OS, CEM, K562, Raji, HeLa cells and PBMC (2 × 10⁶ cells/ml) were cultured with LPS from E.coli serotype O111:B4 (Sigma-Aldrich) at 0.1, 1, 10 μg/ml; or only the vehicle as control, for 24 h.

Molina M.L., Guerrero J., Cidlowski J.A., Gatica H, & Goecke A. (2017). LPS regulates the expression of glucocorticoid receptor α and β isoforms and induces a selective glucocorticoid resistance in vitro. Journal of Inflammation (London, England), 14, 22.

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Endotoxemia Mouse Model Using E. coli LPS

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We used a mouse model of endotoxemia that consisted of intraperitoneal injection of a high dose of Escherichia coli LPS (5 mg/kg) (E. coli serotype O111:B4; Sigma). For more information, please see supporting information.

Xiao J., Zhang B., Su Z., Liu Y., Shelite T.R., Chang Q., Qiu Y., Bei J., Wang P., Bukreyev A., Soong L., Jin Y., Ksiazek T., Gaitas A., Rossi S.L., Zhou J., Laposata M., Saito T.B, & Gong B. (2021). Intracellular receptor EPAC regulates von Willebrand factor secretion from endothelial cells in a PI3K-/eNOS-dependent manner during inflammation. The Journal of Biological Chemistry, 297(5), 101315.

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