Anti human cd11a

Manufactured by BioLegend

Anti-human CD11a is a cell surface glycoprotein that is part of the integrin family. It functions as the alpha subunit of the lymphocyte function-associated antigen 1 (LFA-1) complex, which plays a role in cell-cell adhesion and signal transduction.

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CD11a (10 citations) Anti-CD11a (6 citations)

3 protocols using anti human cd11a

Detailed Immunological Antibody Protocols

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The antibodies used in this study for flow cytometry include: anti-mouse CD8 (#563786), anti-mouse CD25 (#558642), anti-mouse TCRβ (#553174), anti-human CD3 (#563798), anti-human CD8 (#563795), and anti-human TCRαβ (#563826) from BD Biosciences; anti-mouse CD4 (#100544), anti-mouse IFN-γ (#505835), anti-human LFA-1 (#363404), anti-human CD11a (#301208), anti-human CD18 (#302114), anti-human CD25 (#302625), and anti-human CD69 (#310920) from BioLegend; anti-mouse CD4 (#17-0042-83), anti-mouse CD4 (#17-0041-83), anti-mouse CD69 (#25-0691-82), anti-mouse TNF (#17-7321-82), anti-mouse IL-2 (#12-7021-82), anti-human CD4 (#17-0048-2), and anti-human CD8 (#11-0088-41) from eBioscience.
The antibodies used for immunoprecipitation and immunoblotting include: anti-Cdc7 (#ab10535), anti-Dbf4 (#ab124707), anti-RNA polymerase II (#ab817) and anti-RNA polymerase II (phospho S2, # ab193468) from Abcam; anti-PLCγ (#610027) and anti-ERK (#610031) from BD Biosciences; anti-LAT (#641102) from BioLegend; anti-GAPDH (#2118), anti-pPLCγ (#2821), anti-ZAP70 (#2709), anti-pZAP70 (#2717), anti-pERK (#4370), anti-pLAT (#3584), anti-pLck (#6943), and anti-NF-κB (#3035) from Cell Signaling; anti pMCM2 S40 (#GTX62847) from GeneTex; anti-MCM2 (#MA5-15895) from Pierce; and anti-Lck (#sc-433) from Santa Cruz.

Chen E.W., Tay N.Q., Brzostek J., Gascoigne N.R, & Rybakin V. (2019). A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Frontiers in Immunology, 10, 1718.

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Immunoprecipitation and Western Blotting of C3aR and CD11a in Activated NK Cells

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NK cells were isolated and activated with IL2 for 96 hours as described above. The cells were collected and washed with ice cold PBS at 4^oC (1500 rpm for 10 minutes). A standard immunoprecipitation protocol was followed as described in a previous publication (25 (link)). 1 μg of anti-human C3aR (Cat# SC-133172, Santa Cruz Biotechnology) and 1 μg of anti-human CD11a (Cat# 350602, Biolegend) were used in 1 mg of total protein for pulldown. Pulled down eluates were processed for Western blotting as described below.
For mouse NK-cell immunoprecipitation, mouse NK cells were treated with IL2 for 96 hours as described. The activated mouse NK cells were processed as described above. Pulldown assays were performed for C3aR using anti-C3aR ((Cat# SC-133172, Santa Cruz Biotechnology). Polyclonal anti CD11a polyclonal antibody (Cat# BS 20370R, Bioss) and Anti C3aR polyclonal antibody (Cat# BS 2955R, Bioss) were used for immunoblotting.

Nandagopal S., Li C.G., Xu Y., Sodji Q.H., Graves E.E, & Giaccia A.J. (2021). C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models. Cancer immunology research, 10(2), 245-258.

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Toxoplasma gondii strain characterization

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T. gondii lines used include GFP-expressing RH-LDMluc (type I, cloned from RH-GFPS65T) [21 (link)], GFP-expressing PTGluc (type II, cloned from ME49/PTG-GFPS65T) [21 (link)] and RFP-expressing PRU-RFP (type II) [22 (link)]. Tachyzoites were maintained by serial 2-day passaging in murine fibroblasts (L929, Sigma-Aldrich) cultured in Dulbecco's modified Eagle's medium (DMEM; Thermofisher scientific) with 10% fetal bovine serum (FBS; Sigma), gentamicin (20 μg/ml; Gibco), glutamine (2 mM; Gibco), and HEPES (0.01 M; Gibco), referred to as complete medium (CM).
Antibodies used include anti-human CD11a, CD11b, CD18, CD49b, mouse IgG1 κ Isotype, rat IgG2b κ Isotype (all BioLegend) and anti-human CD29 (R&D Systems). All antibodies were added at a concentration of 10 μg/ml in the assays.

Kanatani S., Uhlén P, & Barragan A. (2015). Infection by Toxoplasma gondii Induces Amoeboid-Like Migration of Dendritic Cells in a Three-Dimensional Collagen Matrix. PLoS ONE, 10(9), e0139104.

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