Following CD25- or mock-depletion and stimulation with S. Typhi-infected B-LCL, cells were stained for mass cytometry with a panel of 22 metal-conjugated mAb to detect both Treg and responder T cells. A table of the mAb used is shown in supplementary materials (S1 Table
Cis platinum
Manufactured by Merck Group
Sourced in United States
Cis-platinum is a chemical compound used in various laboratory applications. It is a platinum-based compound with a specific chemical structure. The core function of cis-platinum is to serve as a versatile tool for researchers and scientists in various fields, such as chemistry, materials science, and biology.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Cycloheximide (chx), by Merck Group (3 mentions)
Propidium iodide, by Merck Group (2 mentions)
Nocodazole, by Merck Group (2 mentions)
Mg132, by Merck Group (2 mentions)
Etoposide, by Merck Group (2 mentions)
Olaparib, by Selleck Chemicals (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Ethylenediaminetetraacetic acid disodium salt, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Ribonuclease a, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
13 protocols using cis platinum
1
Mass Cytometry Analysis of Treg and Effector T Cells
2
Agarose Gel Electrophoresis Reagents
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Agarose (normal melting and low melting) was purchased from FMC (Houston, USA). Boric acid was purchased from BDH Chemicals (Dawsonville, GA, USA). Dimethyl sulfoxide, ethylenediaminetetraacetic acid-disodium salt, ethidium bromide, trizma base, triton-X-100, cis-platinum, propidium iodide, ribonuclease A, and paraformaldehyde were purchased from Sigma. Ethanol, hydrochloric acid, sodium hydroxide are purchased from Honeywell-Riedel-de Haën (Seelze, Germany). Sodium chloride, topoisomerase and trypsin were purchased from Gibco BRL (USA). Sodium sarcosinate was purchased from Fluka Biochemika (Germany).
3
Handling of Drugs in Cell Experiments
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Drugs were treated as described in Geva-Zatorsky et al. 2011 [50 (link)]. Cisplatinum (P4394 Sigma) was dissolved in DMSO (hybri-max, D2650 Sigma), giving a stock solution of 25 mM; Nocodazole (M1404, Sigma) was dissolved in DMSO, giving a stock solution of 10 mM; Camptothecin (CPT, C9911 Sigma) and Etoposide (E1383 Sigma) were dissolved in DMSO, giving a stock solution of 0.3 mM; Carboplatin (C2538 Sigma) was dissolved in DDW, giving a stock solution of 500 mM; and MG132 (C2211 Sigma) was dissolved in DMSO, giving a stock solution of 0.32 mM. In each experiment, each drug was diluted to the desired concentration in transparent growth medium (RPMI 1640, 0.05% Penicillin-Streptomycin antibiotics, 10% FCS, with L-Glutamine, lacking riboflavin and phenol red, Bet Haemek, Biological Industries, catalog number 06-1100-26-1A). Normal transparent growth medium was replaced by the diluted drug solution [50 (link)].
4
Maintenance of Embryonal and Testicular Cell Lines
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Human Embryonal Carcinoma cell line NTERA-2 and the murine testicular cell lines TM4 (Sertoli), GC-1 (Spermatogonia), and GC-2 (Spermatocytes) were maintained in DMEM (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Italia), 1% L- Glutamine and 1% of penicillin – streptomycin (Gibco, Paisley, UK) [25] , [26] (link), [27] (link), [28] (link). Olaparib (AZD2281) and P005091 were provided by SelleckChem. Cycloheximide, cisplatinum, H2O2 (H1009), erastin (E7781), ferrostatin-1 (SML0583) and desferoxamine (D9533) were from Sigma-Aldrich, Inc (St. Louis, CA, USA). Z-VAD-fmk (FMK001) was from MedChemExpress.
5
DNA Damage Response Assay in Cell Lines
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HEK 293T, Rad18−/− HCT116 (Shiomi et al., 2007 (link)), and U2OS cells were grown in high glucose DMEM (Gibco) with penicillin/streptomycin (Gibco), glutamine (Gibco), and 10% fetal bovine serum. Cells were damaged with MMS (Sigma-Aldrich), mitomycin C (Sigma-Aldrich), EMS (Sigma-Aldrich), 4-nitroquinoline 1-oxide (Sigma-Aldrich), aphidicolin (Sigma-Aldrich), camptothecin (Sigma-Aldrich), actinomycin D (EMD Millipore), etoposide (Sigma-Aldrich), hydrogen peroxide (Sigma-Aldrich), or cis-platinum (Sigma-Aldrich) at the indicated concentrations and times. Cells were also treated with ATR inhibitor (ATR-45; Charrier et al., 2011 (link)), ATM inhibitor (KU55933; Abcam), MG132 (Sigma-Aldrich), or LLnL (EMD Millipore) at the indicated concentrations and times.
6
Cytotoxicity Assays Reagents Purchase
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Trifluoroacetic acid (TFA), propidium iodide (PI), 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyletrazolium bromide (MTT), cis-platinum, taxol, all trans-retinoic acid (ATRA), and 10 hydroxyl-camptothecin (HCPT) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Cell culture medium RPMI 1640 and fetal bovine serum (FBS) were purchased from GIBCO (Carlsbad, CA, USA). Penicillin-streptomycin and trypsin-versene mixture were purchased from BioWhittaker (Walkersville, MD, USA). Sterilized cell culture materials were purchased from Beckton Dickinson Labware (Franklin Labkes, NJ, USA).
7
Cell Culture Protocols for Cancer Research
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The human cell line NCI-H1975 was provided by Professor Fortunato Ciardiello and was cultured in RPMI 1640 (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK), 1% penicillin/streptomycin (Gibco, Paisley, UK). No RET/PTC1 fusion or CCDC6 mutations have been reported in this cell line [31 (link)]. HeLa Kyoto S-tag-GFP-CCDC6 cells were generated and kindly provided by Ina Poser and Anthony Hyman [6 (link),67 (link)], and were grown in DMEM (high glucose), 10% FCS, 1% penicillin/streptomycin (Gibco, Paisley, UK). 293 AJ2 cells were obtained by Professor Spiros Linardopoulos and were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Paisley, UK) supplemented with 10% fetal calf serum (FCS), 2.4 mM L-glutamine, 100 U/mL penicillin, and 100 µg/ml streptomycin. Cycloheximide was obtained from SIGMA-Aldrich, Inc. (St. Louis, MO, USA). Olaparib was provided by SelleckChem (AZD2281). Hoechst 33258 and cisplatinum were from SIGMA-Aldrich, Inc (St. Louis, MO, USA).
8
Establishing DOX-resistant Ovarian Cancer Cell Line
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MPEG-PLA (MPEG:PLA molar ratio =50:50, molecular weight =4,000 g/mol) was purchased from Daigang (Jinan, China). DOX, cis-platinum (DDP), paclitaxel (PTX), verapamil and CQ were purchased from Sigma (San Francisco, CA, USA). Human ovarian cancer cells A2780 and SKOV3 were purchased from American Type Culture Collection (Rockville, MD, USA) and supplemented with RPMI-1640 complete culture medium (Gibco, Carlsbad, CA, USA) containing 10% fetal calf serum (Gibco), at 37°C in a 5% CO2 atmosphere. The DOX-resistant ovarian cancer cell lines A2780/DOXR were established by culturing cells with DOX in a dose-escalation manner using long-term exposure intervals. Initial cultures of A2780 were supplemented with 10 nM DOX. After sensitive clones were no longer present and surviving A2780 cells repopulated the flask, the concentration of DOX was increased to 25, 50, 100, 200 and 500 nM. The process of acquired drug resistance took 4 months for A2780/DOXR cells.
9
Cytotoxicity Evaluation of TQ and Cisplatin
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Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (Grand Island, USA). TQ, Cis-platinum and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium (MTT) were purchased from Sigma (St Louis, MO, USA). The Annexin V and propidium iodine (PI) kits were supplied by Biovision (USA). The GP-293 and ACHN cell lines were obtained from the Pasteur Institute (Tehran, Iran) and kept in an incubator containing 5% CO2 at 37°C. The cell lines were cultured in DMEM with 10% FBS and 1% penicillin plus streptomycin antibiotic.
10
Cell Culture Conditions for Cancer Research
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HCT116 wild type, FBXW7−/− and USP7−/−, generated by Dr Bert Vogelstein and obtained by the GRCF Cell Center and BioRepository, Baltimore, MD, were grown in McCoy's 5A media (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, 2mM L-glutamine (Gibco, Paisley, UK) [28 (link), 32 (link)]. The human cell lines H1975, H1299, H460, A549 were cultured in RPMI 1640 (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK), 1% penicillin/streptomycin (Gibco, Paisley, UK). No RET/PTC1 fusion or CCDC6 mutations have been reported in these cell lines [45 (link)]. HeLa Kyoto S-tag-GFP-CCDC6 cells were generated by Ina Poser in the laboratory of Anthony Hyman [23 (link)], and were grown in DMEM (high glucose), 10% FCS, 1% penicillin/streptomycin (Gibco, Paisley, UK).
Nocodazole, thymidine, SB-216763 and Cycloheximide were obtained from SIGMA-Aldrich, Inc. Deoxycytidine hydrochloride was from Fluka. MG132 and RO3306 were obtained from Calbiochem (Darmstadt, Germany). Olaparib was provided by SelleckChem (AZD2281) and cisplatinum was from SIGMA-Aldrich, Inc. Okadaic acid was from Biomol International (Farmingdale, New York).
Nocodazole, thymidine, SB-216763 and Cycloheximide were obtained from SIGMA-Aldrich, Inc. Deoxycytidine hydrochloride was from Fluka. MG132 and RO3306 were obtained from Calbiochem (Darmstadt, Germany). Olaparib was provided by SelleckChem (AZD2281) and cisplatinum was from SIGMA-Aldrich, Inc. Okadaic acid was from Biomol International (Farmingdale, New York).
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