Cryosections 5 µm thick were fixed in formalin. Endogenous peroxidase was quenched with 3% hydrogen peroxidase in distilled water. Non-specific binding was blocked using TNB buffer (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% (w/v) blocking reagent, PerkinElmer FP1020). Sections were incubated in Rabbit polyclonal anti-ferritin antibody (Santa Cruz, Cat#sc-25617) or a Rabbit IgG control (Abcam, Cat#ab27478) at a dilution of 2 μg/mL. To visualise the distribution of ferritin, sections were incubated with ImmPRESSTM HRP anti-rabbit IgG (Vector Laboratories, Cat#MP-7401) and 3, 3’-diaminobenzidine (DAKO, Cat# K3468) according to the manufacturer's instructions. Sections were counterstained in haematoxylin before coverslipping.
Immpresstmhrp anti rabbit igg
Manufactured by Vector Laboratories
Sourced in United States
The ImmPRESSTMHRP anti-rabbit IgG is a laboratory equipment product from Vector Laboratories. It is a secondary antibody conjugated with horseradish peroxidase (HRP) that specifically binds to rabbit immunoglobulin G (IgG).
Automatically generated - may contain errors
Lab products found in correlation
Bloxall, by Vector Laboratories (3 mentions)
Immpact sg peroxidase kits, by Vector Laboratories (3 mentions)
Immpact dab peroxidase kit, by Vector Laboratories (2 mentions)
Immpresstmhrp anti mouse igg clone 238446, by Vector Laboratories (2 mentions)
Fp1020, by PerkinElmer (1 mentions)
Control rabbit igg, by Abcam (1 mentions)
3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions)
Ab52632, by Abcam (1 mentions)
Keap1, by Proteintech (1 mentions)
Goat anti rabbit igg secondary antibody, by Boster Bio (1 mentions)
Smurf1, by Abcam (1 mentions)
Bcl 2, by Abmart (1 mentions)
Alexa fluor 555 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alpha tubulin, by Abcam (1 mentions)
Histone h2b, by Santa Cruz Biotechnology (1 mentions)
Smurf1, by Santa Cruz Biotechnology (1 mentions)
Myc tag, by Proteintech (1 mentions)
Gfp tag, by Proteintech (1 mentions)
Eif2α, by ABclonal (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
5 protocols using immpresstmhrp anti rabbit igg
1
Immunohistochemical Detection of Ferritin
2
Dual Immunohistochemical Staining of ITGAX and ITGAM
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After following the hydration protocol described above, the tissues were subjected to heated antigen retrieval in Tris/EDTA buffer (pH 9.0), followed by blocking in BLOXALL (30 min) and 10% goat serum in PBS (20 min) obtained from Vector Labs (Burlingame, CA) and detection of rabbit anti-ITGAX (1:100, 4°C, overnight) (Abcam, ab52632; Cambridge, MA) using ImmPRESSTMHRP anti-rabbit IgG and ImmPACT DAB Peroxidase kits from Vector Labs. The process was repeated for the detection of mouse anti-ITGAM (1:500, 4°C, overnight) (R&D Systems, MAB16991; Minneapolis, MN), employing ImmPRESSTMHRP anti-mouse IgG (clone #238446) and ImmPACT SG Peroxidase kits from Vector Labs. Tissues were also stained with and without primary and secondary antibodies for both antigens to serve as staining controls.
3
Comprehensive Immunoblotting Antibody Panel
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Primary antibodies used against proteins were as follow: SMURF1 (Santa Cruz, sc-100616), SMURF1 (Abcam, ab57573), BiP/GRP78 (ABclonal, A0241); Actin (Sigma, A1978), p62 (MBL, PM045); LC3B (Sigma, L7543); Phospho-IRE1(S724) (ABclonal, AP0878); IRE1(ABclonal, A17940); Phospho-JNK1/2/3 (T183+T183+T221) (Abmart, T55541); JNK (ABclonal, A4867); Phospho-eIF2α (Ser51) (ABclonal, AP0692); eIF2α (ABclonal, A0764); XBP1 (ABclonal, A1731); ATF4 (Santa Cruz, sc-390063); CHOP (ABclonal, A6504); BCL-2 (ABmart, T40056); Flag M2 (Sigma, F3165); GFP-tag (Proteintech, 66002-1-lg); HA-tag (MBL, M180-3); Myc-Tag (Proteintech, 16286-1-AP); NRF2 (Proteintech, 16396-1-AP); KEAP1 (Proteintech, 10503-2-AP); Ubiquitin (MBL, D058-3); alpha Tubulin (Abcam, ab7291), Histone H2B (Santa Cruz, sc-515808); Caspase3 (Santa Cruz, sc-7272); Ki67 (Abcam, ab16667).
Secondary antibodies used were as follow: goat anti-mouse IgG secondary antibody (BOSTER, BA1050); goat anti-rabbit IgG secondary antibody (BOSTER, BA1054); Alexa Fluor® 555 goat anti-mouse IgG (Life Technologies, A21425); ImmPRESSTM HRP anti-Rabbit IgG (VECTOR, MP-7401); ImmPRESSTM HRP anti-Mouse IgG (VECTOR, MP-7402); Rabbit anti-mouse IgG (CST, 58802) and Mouse anti-Rabbit IgG (CST, 93702) were used to avoid interference of the IgG heavy chain.
Secondary antibodies used were as follow: goat anti-mouse IgG secondary antibody (BOSTER, BA1050); goat anti-rabbit IgG secondary antibody (BOSTER, BA1054); Alexa Fluor® 555 goat anti-mouse IgG (Life Technologies, A21425); ImmPRESSTM HRP anti-Rabbit IgG (VECTOR, MP-7401); ImmPRESSTM HRP anti-Mouse IgG (VECTOR, MP-7402); Rabbit anti-mouse IgG (CST, 58802) and Mouse anti-Rabbit IgG (CST, 93702) were used to avoid interference of the IgG heavy chain.
4
Immunohistochemical Detection of ITGB2 and ICAM-3
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After following the hydration protocol described above, the tissues were subjected to heated antigen retrieval in citrate buffer (pH 6.0), blocking in BLOXALL (30 min) and 10% goat serum in PBS (20 min) obtained from Vector Labs (Burlingame, CA), and detection of rabbit anti-ITGB2 (1:100, 4°C, overnight) (Abcam, ab131044; Cambridge, MA) using ImmPRESSTMHRP anti-rabbit IgG and ImmPACT DAB Peroxidase kits from Vector Labs. The process was repeated for the detection of mouse anti-ICAM-3 (1:1000, 4°C, overnight) (Abcam, ab10804; Cambridge, MA), employing ImmPRESSTMHRP anti-mouse IgG (clone ab10804) and ImmPACT SG Peroxidase kits from Vector Labs. Tissues were also stained with and without primary and secondary antibodies for both antigens to serve as staining controls.
5
Immunohistochemical Analysis of CCR7 and CCL19
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Slide-mounted anterior ethmoid tissues were sequentially deparaffinized in xylenes and rehydrated in ethanol (Thermo Fisher Scientific; Pittsburg, PA) and ddH2O, as previously described.30 (link) Tissues were then subjected to heated-antigen retrieval in citrate-based antigen unmasking solution (pH 6.0), followed by antigen blocking with BLOXALL (20 min) and 2.5% goat serum (20 min), all of which were obtained from Vector Laboratories (Burlingame, CA, USA). The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESSTMHRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA). This staining protocol was repeated for monoclonal mouse anti-human CCL19 (1:150, 4°C, overnight) (Invitrogen, OTI2A12; Carlsbad, CA, USA) detection, employing heated antigen retrieval with Tris-based antigen unmasking solution, ImmPRESSTMHRP anti-mouse IgG (clone #238446), and ImmPACT SG Peroxidase kits from Vector Laboratories.
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