Ch200

Manufactured by Randox

Sourced in United Kingdom

The CH200 is a clinical chemistry analyzer designed for in-vitro diagnostic testing. It is capable of performing a variety of biochemical assays to measure different analytes in biological samples. The CH200 is engineered to provide accurate and reliable results, supporting healthcare professionals in their diagnostic decision-making processes.

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Lab products found in correlation

Tr0100, by Merck Group (2 mentions) Tr 1697, by Randox (2 mentions) Tr210, by Randox (2 mentions) Autokit 3 hb, by Fujifilm (1 mentions) Nefa hr, by Fujifilm (1 mentions) 80 insms e01, by ALPCO (1 mentions) Onetouch, by LifeScan (1 mentions) Gpo pap, by Randox (1 mentions) Gl364, by Randox (1 mentions) Mithras microplate reader, by Berthold Technologies (1 mentions) Adviaxpt 2400, by Siemens (1 mentions) Sircol soluble collagen assay kit, by Biocolor (1 mentions) Ch203, by Randox (1 mentions)

7 protocols using ch200

Comprehensive Metabolic Profiling Assay

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Blood glucose levels were determined using an automatic glucose monitor (One Touch, LifeScan). In addition, commercially available kits were used to measure serum non‐esterified fatty acids (NEFA; NEFA‐HR, Wako), glycerol/triglyceride (TG; TR‐0100; Sigma‐Aldrich), ketone bodies (KB; Autokit 3‐HB, Wako), cholesterol (CH200, Randox) and insulin (80‐INSMS‐E01, Alpco) essentially according to manufacturer's instructions. All samples were loaded in order to fit within the assay range of the reagents supplied. Acylcarnitines were determined in serum by electrospray ionisation tandem mass spectrometry (ESI‐MS/MS) according to a modified method as previously described (Sauer et al, 2006), using a Quattro Ultima triple quadrupole mass spectrometer (Micromass, Manchester, UK) equipped with an electrospray ion source and a Micromass MassLynx data system.

Fuhrmeister J., Zota A., Sijmonsma T.P., Seibert O., Cıngır Ş., Schmidt K., Vallon N., de Guia R.M., Niopek K., Berriel Diaz M., Maida A., Blüher M., Okun J.G., Herzig S, & Rose A.J. (2016). Fasting‐induced liver GADD45β restrains hepatic fatty acid uptake and improves metabolic health. EMBO Molecular Medicine, 8(6), 654-669.

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Enzymatic Determination of Cholesterol and Triglycerides

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Total cholesterol and triglyceride levels were determined with an enzymatic colorimetric test from commercially available kits (CHOL, Randox, CH200; GPO-PAP, Randox, No. Cat. TR210), in accordance with the manufacturer's instructions [26 (link), 31 (link)].

Alemán-González-Duhart D., Álvarez-Almazán S., Valdes M., Tamay-Cach F, & Mendieta-Wejebe J.E. (2021). In Vivo and Ex Vivo Evaluation of 1,3-Thiazolidine-2,4-Dione Derivatives as Euglycemic Agents. PPAR Research, 2021, 5100531.

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Spectrophotometric Analysis of Serum Analytes

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Concentrations of glucose, cholesterol and triacylglycerol were measured spectrophotometrically using a commercially available enzymes assay kits (GL 364, Randox, Crumlin, UK, for glucose; CH 200, Randox, Crumlin, UK for cholesterol and TR 1697, Randox, Crumlin, UK for triacylglycerol). Concentrations of calcium and phosphate in serum were measured spectrophotometrically (using Arsenazo III method for calcium and UV for phosphate) with authomatic biochemical analyzer (Biosystem A15; Biosystems, Spain).

Nikolić T., Petronijević M., Sopta J., Velimirović M., Stojković T., Jevtić Dožudić G., Aksić M., Radonjić N.V, & Petronijević N. (2017). Haloperidol affects bones while clozapine alters metabolic parameters - sex specific effects in rats perinatally treated with phencyclidine. BMC Pharmacology & Toxicology, 18, 65.

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Biomarkers of Metabolic Homeostasis

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Circulating levels of triglycerides, total cholesterol and free fatty acids were determined with TR210, CH200 and FA115 Randox kits, respectively, following standard protocols according to manufacturer’s recommendations (Laboratoires Randox, Roissy, France). Leptin levels were determined using a commercial OKAA00022 ELISA kit, according to manufacturer’s instructions (Aviva Systems Biology, San Diego, CA).

Henriques A., Blasco H., Fleury M.C., Corcia P., Echaniz-Laguna A., Robelin L., Rudolf G., Lequeu T., Bergaentzle M., Gachet C., Pradat P.F., Marchioni E., Andres C.R., Tranchant C., Gonzalez De Aguilar J.L, & Loeffler J.P. (2015). Blood Cell Palmitoleate-Palmitate Ratio Is an Independent Prognostic Factor for Amyotrophic Lateral Sclerosis. PLoS ONE, 10(7), e0131512.

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Quantification of Liver Lipids and Collagen

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Triglycerides (TR0100, Sigma Aldrich) and total cholesterol (CH200, Randox, Crumlin, UK) were measured in serum and liver lipid lysates using the Mithras Microplate Reader (Berthold Technologies GmbH & Co, Bad Wildbad, Germany) at 550 nm. Liver lipids were extracted as described previously [15 ]. Serum levels of HDL-cholesterol were analyzed with an AdviaXPT® 2400 clinical chemistry automation (Siemens Healthineers) according to the standard operating protocol. Sircol soluble collagen assay kit (Biocolor life science assays) was used to quantify collagen in mouse liver tissue according to the manufacturer's instructions. Briefly, approximately 25–50 mg of liver material was digested with pepsin overnight at 4 °C. Neutralized samples were concentrated and mixed with Sirius red dye reagent for 30 min before washing and centrifugation. Alkali reagent was added to release collagen bound dye into solution. Samples were measured on the Mithras Microplate Reader at 555 nm.

Wolff G., Sakurai M., Mhamane A., Troullinaki M., Maida A., Deligiannis I.K., Yin K., Weber P., Morgenstern J., Wieder A., Kwon Y., Sekar R., Zeigerer A., Roden M., Blüher M., Volk N., Poth T., Hackert T., Wiedmann L., De Angelis Rigotti F., Rodriguez-Vita J., Fischer A., Mukthavaram R., Limphong P., Tachikawa K., Karmali P., Payne J., Chivukula P., Ekim-Üstünel B., Martinez-Jimenez C.P., Szendrödi J., Nawroth P, & Herzig S. (2022). Hepatocyte-specific activity of TSC22D4 triggers progressive NAFLD by impairing mitochondrial function. Molecular Metabolism, 60, 101487.

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Plasma Lipid and Enzyme Profiling

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Plasma triacylglycerol (cat. no. TR210, Randox, Crumlin, UK), total cholesterol (cat. no. CH200, Randox), aspartate aminotransferase (AST, cat. no. MAK055, Sigma-Aldrich, Milan, Italy), and alanine aminotransferase (ALT, cat. no. MAKO52, Sigma-Aldrich) analyses were performed using commercially available kits, according to the manufacturer’s instructions.

Giudetti A.M., Vergara D., Longo S., Friuli M., Eramo B., Tacconi S., Fidaleo M., Dini L., Romano A, & Gaetani S. (2021). Oleoylethanolamide Reduces Hepatic Oxidative Stress and Endoplasmic Reticulum Stress in High-Fat Diet-Fed Rats. Antioxidants, 10(8), 1289.

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Enzymatic Determination of Plasma Lipids

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Plasma total cholesterol (TC), HDL-C, and triglycerides (TG) were determined using commercially available enzymatic kits (CH 200, CH 203, TR 1697 respectively, Randox Laboratories Ltd., Crumlin, UK). HDL-C was measured in the supernatant, after the treatment of plasma with a phosphotungstate reagent, as previously described [15 (link)]. Non-HDL-C, which includes LDL-C, was calculated as the difference between TC and HDL-C.

Romain C., Piemontese A., Battista S., Bernini F., Ossoli A., Strazzella A., Gaillet S., Rouanet J.M., Cases J, & Zanotti I. (2018). Anti-Atherosclerotic Effect of a Polyphenol-Rich Ingredient, Oleactiv®, in a Hypercholesterolemia-Induced Golden Syrian Hamster Model. Nutrients, 10(10), 1511.

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