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Simoa nf light advantage sr x kit

Manufactured by Quanterix
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Sourced in United States

The Simoa® NF-light™ Advantage (SR-X) Kit is a laboratory equipment product designed for the measurement of NF-light protein concentration in biological samples. The kit utilizes Simoa® technology, a highly sensitive and quantitative detection method, to enable accurate and precise measurement of the target protein.

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Lab products found in correlation

Sr x platform, by Quanterix (2 mentions) Microtainer blood collection tube, by BD (2 mentions) Sr x instrument, by Quanterix (2 mentions) Simoa sr x analyzer, by Quanterix (1 mentions)

5 protocols using simoa nf light advantage sr x kit

1

Plasma NFL Quantification in Mice

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Plasma samples were collected from PPIA+/+ and PPIA−/− mice in K2-EDTA BD Microtainer blood collection tube and centrifuged at 5000g for 5 min to isolate plasma sample. Plasma neurofilament light chain (NFL) concentration was measured using the Simoa® NF-light™ Advantage (SR-X) Kit (no. 103400) on the Quanterix SR-X™ platform with reagents from a single lot, according to the protocol issued by the manufacturer (Quanterix Corp).
Pasetto L., Grassano M., Pozzi S., Luotti S., Sammali E., Migazzi A., Basso M., Spagnolli G., Biasini E., Micotti E., Cerovic M., Carli M., Forloni G., De Marco G., Manera U., Moglia C., Mora G., Traynor B.J., Chiò A., Calvo A, & Bonetto V. (2021). Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia. Brain, 144(12), 3710-3726.
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2

Quantification of Neurofilament Light in CSF and Serum

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NfL levels in CSF and serum were measured using a Simoa SR-X analyzer (Quanterix Corporation, Massachusetts) with a Simoa NF-light Advantage (SR-X) Kit (Quanterix, No. 103400) according to the manufacturer’s instructions.
Sano T., Masuda Y., Yasuno H., Shinozawa T., Watanabe T, & Kakehi M. (2021). Blood Neurofilament Light Chain as a Potential Biomarker for Central and Peripheral Nervous Toxicity in Rats. Toxicological Sciences, 185(1), 10-18.
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3

Plasma NF-light Quantification using Simoa

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Plasma samples were measured in duplicates with commercially available Simoa NF-light Advantage (SR-X) kit (Quanterix, cat. 103400). Samples were diluted 4:1 and incubated with 25 µl anti-NF-light immunocapture beads and 20 µl biotinylated detector antibody at 30 °C and 800 rpm for 30 min. Following the incubation, the bead-immunocomplexes were washed and resuspended before being incubated with 100 µl streptavidin-labelled β-galactosidase at 30 °C and 800 rpm for 10 min. After a second washing step, the bead-immunocomplexes and resorufin β-d-galactopyranoside were loaded onto an SR-X instrument (Quanterix) for processing and analysis.
Eldjarn G.H., Ferkingstad E., Lund S.H., Helgason H., Magnusson O.T., Gunnarsdottir K., Olafsdottir T.A., Halldorsson B.V., Olason P.I., Zink F., Gudjonsson S.A., Sveinbjornsson G., Magnusson M.I., Helgason A., Oddsson A., Halldorsson G.H., Magnusson M.K., Saevarsdottir S., Eiriksdottir T., Masson G., Stefansson H., Jonsdottir I., Holm H., Rafnar T., Melsted P., Saemundsdottir J., Norddahl G.L., Thorleifsson G., Ulfarsson M.O., Gudbjartsson D.F., Thorsteinsdottir U., Sulem P, & Stefansson K. (2023). Large-scale plasma proteomics comparisons through genetics and disease associations. Nature, 622(7982), 348-358.
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4

Plasma NF-light Quantification using Simoa

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Plasma samples were measured in duplicates with commercially available Simoa NF-light Advantage (SR-X) kit (Quanterix, cat. 103400). Samples were diluted 4:1 and incubated with 25 µl anti-NF-light immunocapture beads and 20 µl biotinylated detector antibody at 30 °C and 800 rpm for 30 min. Following the incubation, the bead-immunocomplexes were washed and resuspended before being incubated with 100 µl streptavidin-labelled β-galactosidase at 30 °C and 800 rpm for 10 min. After a second washing step, the bead-immunocomplexes and resorufin β-d-galactopyranoside were loaded onto an SR-X instrument (Quanterix) for processing and analysis.
Eldjarn G.H., Ferkingstad E., Lund S.H., Helgason H., Magnusson O.T., Gunnarsdottir K., Olafsdottir T.A., Halldorsson B.V., Olason P.I., Zink F., Gudjonsson S.A., Sveinbjornsson G., Magnusson M.I., Helgason A., Oddsson A., Halldorsson G.H., Magnusson M.K., Saevarsdottir S., Eiriksdottir T., Masson G., Stefansson H., Jonsdottir I., Holm H., Rafnar T., Melsted P., Saemundsdottir J., Norddahl G.L., Thorleifsson G., Ulfarsson M.O., Gudbjartsson D.F., Thorsteinsdottir U., Sulem P, & Stefansson K. (2023). Large-scale plasma proteomics comparisons through genetics and disease associations. Nature, 622(7982), 348-358.
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5

Plasma NFL Concentration Measurement

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Plasma samples were collected from PPIA+/+ and PPIA-/-mice in K2-EDTA BD Microtainer blood collection tube and centrifuged at 5000 xg for 5 minutes to isolate plasma sample. The plasma NFL concentration was measured using the Simoa® NF-light™ Advantage (SR-X) Kit (#103400) on the Quanterix SR-X™ platform with reagents from a single lot, according to the protocol issued by the manufacturer (Quanterix Corp, Boston, MA, USA).
Pasetto L., Grassano M., Pozzi S., Luotti S., Sammali E., Migazzi A., Basso M., Spagnolli G., Biasini E., Micotti E., Cerovic M., Carli M., Forloni G., Marco G.D., Manera U., Moglia C., Mora G., Traynor B.J., Chiò A., Calvo A., & Bonetto V. (2020). Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia.
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