Cary model 100 spectrometer

Manufactured by Agilent Technologies

The Cary Model 100 spectrometer is a UV-Vis absorption spectrophotometer designed for routine analytical applications. It measures the absorbance or transmittance of a sample across the ultraviolet and visible light spectrum.

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Lab products found in correlation

Cary eclipse fluorometer, by Agilent Technologies (6 mentions) Quartz cuvette, by Starna Cells (6 mentions) Quartz microcuvette, by Hellma (2 mentions) Cary eclipse, by Agilent Technologies (2 mentions) Quantaurus qy spectrometer, by Hamamatsu Photonics (2 mentions) Quartz microcuvettes, by Starna Cells (2 mentions)

Spelling variants of this product

Cary 100 (244 citations) Cary 100 spectrometer (12 citations) Spectrometers (10 citations)

8 protocols using cary model 100 spectrometer

Spectroscopy and Imaging Methodology

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Example 14

All measurements were taken at ambient temperature (23±2° C.). Fluorescent molecules were prepared as stock solutions in DMSO and diluted such that the DMSO concentration did not exceed 1% v/v. Spectroscopy was performed using 1-cm path length quartz cuvettes (Starna). Absorption measurements were recorded on a Cary Model 100 spectrometer (Varian). Fluorescence measurements spectra were recorded on a Cary Eclipse fluorometer (Varian). Images were processed in ImageJ/Fiji. Data was analyzed and graphs were plotted using Prism (GraphPad). Movie renderings were done using Imaris (Bitplane)

US11498932B2. Bright targetable red CA²⁺ indicators (2022-11-15). HOWARD HUGHES MEDICAL INSTITUTE [US]. Inventors: Luke D. Lavis [US], Claire Deo [DE].

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Spectroscopic Characterization of Biomolecules

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Spectroscopy was performed using 1-cm path length, 3.5-mL quartz cuvettes from Starna Cells or 1-cm path length, 1.0-mL quartz microcuvettes from Hellma. All measurements were taken at ambient temperature (22 ± 2 °C) in 10 mM HEPES, pH 7.3 buffer unless otherwise noted. Absorption spectra were recorded on a Cary Model 100 spectrometer (Varian); reported values for extinction coefficients (ε) are averages (n = 3). Fluorescence spectra were recorded on a Cary Eclipse fluorometer (Varian). Normalized spectra are shown for clarity.

Grimm J.B., English B.P., Chen J., Slaughter J.P., Zhang Z., Revyakin A., Patel R., Macklin J.J., Normanno D., Singer R.H., Lionnet T, & Lavis L.D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature methods, 12(3), 244-250.

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Spectroscopic Characterization of Compounds

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Spectroscopy was performed using 1-cm path length, 3.5-mL quartz cuvettes or 100-µL quartz microcuvettes Starna Cells. All measurements were taken at ambient temperature (~ 22 °C). Absorption spectra were recorded on a Cary Model 100 spectrometer (Varian), and fluorescence spectra were recorded on a Cary Eclipse fluorometer (Varian). The pK_a values for compounds 1, 2, and 3 were determined in buffers containing 150 mM NaCl and 10 mM buffer. The following buffer systems were used: citrate (pH 4.0–6.2); phosphate (pH 5.8–8.0); tris (pH 7.8–9.0); carbonate (pH 9.2–10.0). Fluorescence values were read on 500 nM samples (n = 3) and fitted to a sigmoidal dose response curve (variable slope) using GraphPad Prism software. Samples for visual inspection containined 5 µM 1 or 2 in citrate (pH 5.6) or phosphate (pH 7.4) buffer.

Martineau M., Somasundaram A., Grimm J.B., Gruber T.D., Choquet D., Taraska J.W., Lavis L.D, & Perrais D. (2017). Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis. Nature Communications, 8, 1412.

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Spectroscopic Characterization of Biomolecules

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Spectroscopy was performed using 1-cm path length, 3.5-mL quartz cuvettes from Starna Cells or 1-cm path length, 1.0-mL quartz microcuvettes from Hellma. All measurements were taken at ambient temperature (22 ± 2 °C) in 10 mM HEPES, pH 7.3 buffer unless otherwise noted. Absorption spectra were recorded on a Cary Model 100 spectrometer (Varian); reported values for extinction coefficients (ε) are averages (n = 3). Fluorescence spectra were recorded on a Cary Eclipse fluorometer (Varian). Normalized spectra are shown for clarity.

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Spectroscopic Characterization of Biomolecules

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Spectroscopy was performed using 1-cm path length, 3.5-mL quartz
cuvettes from Starna Cells or 1-cm path length, 1.0-mL quartz microcuvettes from
Hellma. All measurements were taken at ambient temperature (22 ± 2
°C). Absorption spectra were recorded on a Cary Model 100 spectrometer
(Agilent). Fluorescence emission spectra were recorded on a Cary Eclipse
(Varian). Absolute fluorescence quantum yields were recorded on a Quantaurus-QY
spectrometer (model C11374, Hamamatsu). All spectroscopy measurements were
performed in phosphate-buffered saline (PBS), pH 7.4 and the values of maximum
absorption wavelength (λ_max), extinction
coefficient at λ_max(ε), maximum absorption wavelength
(λ_em), and fluorescence quantum yield
(Φ_f) are averages (n=3; Supplementary Fig.
1k).

Banala S., Arvin M.C., Bannon N.M., Jin X.T., Macklin J.J., Wang Y., Peng C., Zhao G., Marshall J.J., Gee K.R., Wokosin D.L., Kim V.J., McIntosh J.M., Contractor A., Lester H.A., Kozorovitskiy Y., Drenan R.M, & Lavis L.D. (2018). Photoactivatable drugs for nicotinic optopharmacology. Nature methods, 15(5), 347-350.

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Fluorescent Molecule Spectroscopy Protocol

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Fluorescent and fluorogenic molecules for spectroscopy were prepared as stock solutions in DMSO and diluted such that the DMSO concentration did not exceed 1% v/v. Spectroscopy was performed using 1-cm path length, 3.5-mL quartz cuvettes or 1-cm path length, 1.0-mL quartz microcuvettes from Starna Cells. All measurements were taken at ambient temperature (22 ± 2 °C). Absorption spectra were recorded on a Cary Model 100 spectrometer (Agilent). Fluorescence spectra were recorded on a Cary Eclipse fluorometer (Varian). Maximum absorption wavelength (λ_abs), extinction coefficient (ε), and maximum emission wavelength (λ_em) were taken in 10 mM HEPES, pH 7.3 buffer unless otherwise noted; reported values for ε are averages (n = 3). Normalized spectra are shown for clarity.

Grimm J.B., Muthusamy A.K., Liang Y., Brown T.A., Lemon W.C., Patel R., Lu R., Macklin J.J., Keller P.J., Ji N, & Lavis L.D. (2017). A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nature methods, 14(10), 987-994.

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Fluorescent Molecules Spectroscopy Protocol

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Fluorescent and fluorogenic molecules for spectroscopy were prepared as stock solutions in DMSO and diluted such that the DMSO concentration did not exceed 1% v/v. Spectroscopy was performed using 1-cm path length, 3.5-mL quartz cuvettes or 1-cm path length, 1.0-mL quartz microcuvettes from Starna Cells. All measurements were taken at ambient temperature (22 ± 2 °C). Absorption spectra were recorded on a Cary Model 100 spectrometer (Agilent). Fluorescence spectra were recorded on a Cary Eclipse fluorometer (Varian). Maximum absorption wavelength (λ_abs), extinction coefficient (ε), and maximum emission wavelength (λ_em) were measured in 10 mM HEPES, pH 7.3 buffer; reported values for ε are averages (n = 3). Normalized spectra are shown for clarity. For prototype ion indicators 32 and 33 (Extended Data Fig. 5d–e) the compounds were dissolved in 10 mM HEPES, pH 7.3 buffer alone or with either 100 mM KCl or 10 μM ZnCl₂; the fluorescence emission spectra of these solutions were recorded using λ_ex = 575 nm and λ_em = 625–825 nm.

Grimm J.B., Tkachuk A.N., Xie L., Choi H., Mohar B., Falco N., Schaefer K., Patel R., Zheng Q., Liu Z., Lippincott-Schwartz J., Brown T.A, & Lavis L.D. (2020). A general method to optimize and functionalize red-shifted rhodamine dyes. Nature methods, 17(8), 815-821.

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Spectroscopic Characterization of Biomolecules

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Spectroscopy was performed using 1-cm path length, 3.5-mL quartz
cuvettes from Starna Cells or 1-cm path length, 1.0-mL quartz microcuvettes from
Hellma. All measurements were taken at ambient temperature (22 ± 2
°C). Absorption spectra were recorded on a Cary Model 100 spectrometer
(Agilent). Fluorescence emission spectra were recorded on a Cary Eclipse
(Varian). Absolute fluorescence quantum yields were recorded on a Quantaurus-QY
spectrometer (model C11374, Hamamatsu). All spectroscopy measurements were
performed in phosphate-buffered saline (PBS), pH 7.4 and the values of maximum
absorption wavelength (λ_max), extinction
coefficient at λ_max(ε), maximum absorption wavelength
(λ_em), and fluorescence quantum yield
(Φ_f) are averages (n=3; Supplementary Fig.
1k).

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