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Ha nh2

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HA-NH2 is a chemical reagent used in laboratory applications. It consists of hyaluronic acid (HA) that has been modified to include amino (NH2) functional groups. The core function of HA-NH2 is to provide a reactive platform for further chemical modifications or conjugations. This product is intended for use in research and development settings.

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Lab products found in correlation

Caspase 9 substrate, by Merck Group (2 mentions) Propidium iodide, by Thermo Fisher Scientific (2 mentions) Cyt c from equine heart, by Merck Group (2 mentions) Nucblue fixed cell readyprobes reagent, by Thermo Fisher Scientific (2 mentions) Caspase colorimetric assay kit, by Abcam (2 mentions) Celltiter 96 aqueous non radioactive cell proliferation assay, by Promega (2 mentions) Atcc ccl 185, by American Type Culture Collection (2 mentions) Cos 7, by American Type Culture Collection (2 mentions)

2 protocols using ha nh2

1

Cyt c-HA-NH2 Conjugate for Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyt c from equine heart was from Sigma-Aldrich® (St. Louis, MO) and HA-NH2 (10 kDa) from Creative PEGWorks (Winston-Salem, NC). The dithiobis(succinimidyl propionate) (DSP) cross linker was obtained from ProteoChem™ (Loves Park, IL). The cell lines A549 (human lung adenocarcinoma; ATCC® CCL185 ™) and COS-7 (African green monkey kidney fibroblast) cells were from the American Type Culture Collection (Manassas, VA). CellTiter 96 aqueous non-radioactive cell proliferation assay was from Promega Corporation (Madison, WI). NucBlue® Fixed Cell ReadyProbes® Reagent (4′,6-diamidino-2-phenylindole, dihydrochloride, DAPI), fluorescein (FITC), Annexin Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and propidium iodide (PI) were purchased from Invitrogen (Carlsbad, CA). Caspase colorimetric assay kit was purchased from BioVision, Inc., San Francisco, CA and the caspase 9 substrate was from Sigma Aldrich (St. Louis, MO). All other chemicals (reagentor analytical grade) were purchased from various suppliers and used without further purification.
Figueroa C., Suárez B., Molina A., Fernández J., Torres Z, & Griebenow K. (2017). Smart Release Nano-formulation of Cytochrome C and Hyaluronic Acid Induces Apoptosis in Cancer Cells. Journal of nanomedicine & nanotechnology, 8(1), 427.
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2

Cyt c-HA-NH2 Conjugate for Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyt c from equine heart was from Sigma-Aldrich® (St. Louis, MO) and HA-NH2 (10 kDa) from Creative PEGWorks (Winston-Salem, NC). The dithiobis(succinimidyl propionate) (DSP) cross linker was obtained from ProteoChem™ (Loves Park, IL). The cell lines A549 (human lung adenocarcinoma; ATCC® CCL185 ™) and COS-7 (African green monkey kidney fibroblast) cells were from the American Type Culture Collection (Manassas, VA). CellTiter 96 aqueous non-radioactive cell proliferation assay was from Promega Corporation (Madison, WI). NucBlue® Fixed Cell ReadyProbes® Reagent (4′,6-diamidino-2-phenylindole, dihydrochloride, DAPI), fluorescein (FITC), Annexin Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and propidium iodide (PI) were purchased from Invitrogen (Carlsbad, CA). Caspase colorimetric assay kit was purchased from BioVision, Inc., San Francisco, CA and the caspase 9 substrate was from Sigma Aldrich (St. Louis, MO). All other chemicals (reagentor analytical grade) were purchased from various suppliers and used without further purification.
Figueroa C., Suárez B., Molina A., Fernández J., Torres Z, & Griebenow K. (2017). Smart Release Nano-formulation of Cytochrome C and Hyaluronic Acid Induces Apoptosis in Cancer Cells. Journal of nanomedicine & nanotechnology, 8(1), 427.
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+ Expand

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