Mouse anti cyclin a2

Western blotting was performed as already described^{55 (link)}. The following antibodies were used: mouse anti-GLI1 (#2643), mouse anti-cyclin A2 (#4656), rabbit anti-BCL2 (#2876), rabbit anti-BAX (#2772), rabbit anti-cyclin B1 (#12231), rabbit anti-PARP-1 (#9532), rabbit anti-phospho-ATR (Ser428) (#2853), rabbit anti-phospho-CHK1 (Ser345) (#2348), rabbit anti-phospho-CDC2 (Tyr15) (#4539), rabbit anti-phospho-H2A.X (Ser139) (#9718), rabbit anti-phospho-Histone H3 (Ser10) (#3377), rabbit anti-phospho-WEE1 (Ser642) (#4910) (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-CDC2 (sc-954), mouse anti-Myc (sc-40), mouse anti-HSP90 (sc-13119) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and rabbit anti-SMO (ST1718) (Merck Millipore, Burlington, MA, USA). Chemiluminescent detection was used. Cell fractionation was performed as previously described^{56 (link)}. The following antibodies were used: mouse anti-GLI1 (#2643) (Cell Signaling Technology), goat anti-fibrillarin (D-14), and goat anti-GAPDH (V-18) (Santa Cruz Biotechnology).

Immunoblotting and RT-qPCR were performed as described before [24 (link)]. The antibodies used for immunoblotting were rabbit anti-A3B, 1:1000 (5210-87-13 [26 (link)]); rabbit anti-Cyclin E2, 1:1000 (Cell Signaling, Danvers, MA, USA, #4132); rabbit anti-Cyclin B1, 1:1000 (Cell Signaling, D5C10); mouse anti-Cyclin A2, 1:2000 (Cell Signaling, BF683); rabbit anti-FoxM1, 1:1000 (Cell Signaling, D12D5); and mouse anti-Tubulin, 1:20,000 (Sigma-Aldrich, T5168). Primers used for RT-qPCR were as follows: A3A: FWD 5′-GAGAAGGGACAAGCACATGG-3′ and REV 5′-TGGATCCATCAAGTGTCTGG-3′; A3B: FWD 5′-GCCACAGAGAAGATTCTTAGCC-3′ and REV 5′-CGCCAGACCTACTTGTGCTA-3′; CCNE2: FWD 5′-TCCAAGAGTTTGCTTACGTCAC-3′ and REV 5′-GCCAGGAGATGATTGTTACAGG-3′; CCNB1: FWD 5′-TCTTGCAGTAAATGATGTGG-3′ and REV 5′-CAGTCAATTAGGATGGCTCT-3′; B2M: FWD 5′-CTTTGTCACAGCCCAAGATAG-3′ and REV 5′-CAATCCAAATGCGGCATCTTC-3′; HPRT: FWD 5′-TGACACTGGCAAAACAATGCA-3′ and REV 5′-GGTCCTTTTCACCAGCAAGCT-3′; TBP: FWD 5′-CCCATGACTCCCATGACC-3′ and REV 5′-TTTACAACCAAGATTCACTGTGG-3′.