Prism v 4

All data are reported as means±standard error of the mean (SEM). Data were analyzed using GraphPad Prism v.4 and SPSS v.16.0 software (Chicago, IL). For plasma corticosterone, body temperature, and c-Fos analyses, 2-way ANOVAs were utilized with drug treatment (MA, saline) and treatment period as between-subject factors. Bonferroni corrected post hoc comparisons were performed when statistically appropriate.

All data are reported as means ± standard error of the mean (SEM). Data were analyzed using GraphPad Prism v.4 and SPSS v.16.0 software (Chicago, IL). For the immunohistochemical analyses, data are presented as the number and percentage of c-Fos positive cells expressing GR or AVP. For corticosterone and body temperature analysis, 3-way ANOVA was utilized with treatment (MA, saline), sex (male, female), and time as between subject factors (0, 30, 70, 120 minutes). Immunohistochemical data (c-Fos, c-Fos/AVP, and c-Fos/GR) were analyzed using two-way ANOVA with sex and treatment as factors as well as separated by sex and analyzed as fold change compared to saline injection. Correlations between corticosterone and body temperature were performed using Spearman’s Rank Correlation Coefficient. Bonferroni corrected post hoc comparisons were performed when statistically appropriate. Student’s T-tests were utilized for comparison of fold increases in c-Fos and c-Fos/GR dual-labeled cells in MA compared to Saline treated mice.