Ms qqq mass spectrometer

The analysis was performed on a MS QQQ mass spectrometer coupled to Aglient 6420 series UPLC system (Agilent Technologies, Waldbronn, Germany), equipped with a 1290 ultra-performance auto sampler, 1290 infinity Quad pump, and 6420 triple quadrupole detector. Chromatographic separation was performed on a Acquity UPLC® HSS T3 (150 mm × 2.1 mm i.d.; 1.8 μm) column (Waters, USA).

To analyze the cycloalliin, sample (or cycloalliin hydrochloride monohydrate as a standard, Fujifilm Wako Pure Chemical Co., Osaka, Japan) was dissolved in methanol at 0.1 g/mL. Agilent 6410 Triple Quad LC/MS (Agilent Technologies, Santa Clara, CA, USA) coupled to a MS QQQ mass spectrometer equipped with an electrospray ionization sources (Agilent Technologies) was used. Chromatographic separations were performed on a reversed phase C18 with polar end-capping (15 mm × 2 mm, Synergi^TM 4 μm Hydro-RP 80Å; phenomenex, Torrance, CA, USA). The operating temperature was set at 30 °C and the flow rate was 0.2 mL/min. Mobile phase A and B consisted of 0.1% formic acid in water and 0.1% formic acid in acetonitrile, respectively. The gradient system was as follows: 0 min, 5% B; 1 min, 5% B; 11 min, 100% B; 12 min, 100% B; 15 min, 5% B; and 20 min, 5% B. The mass spectrometer was operated using MS QQQ mass spectrometer for electrospray ionization (ESI). The general settings used were as follows: gas temperature, 300 °C; gas flow, 11 L/min; nebulizer, 15 psi; capillary, 4000 V. Regression equation (y) is 233.26x + 259.29 and correlation coefficient (R2) is 1.