Blood was recovered in Vacutainer glass collection tubes with heparin (BD Biosciences). PBMC were isolated by gradient centrifugation with Histopaque-1077. Plasma was recovered for cytokine quantification by cytokine bead array (BD Biosciences) and resistin ELISA (Peprotech). Cell aliquots were frozen in liquid nitrogen. Following blood draw, PBMC isolation was performed within 24 hours through density gradient centrifugation, and cells were stored immediately in liquid nitrogen. Flow cytometry characterization of PBMC involved incubation with Human TruStain FcX™(Biolegend) and staining with primary antibodies: CD14(HCD14, Biolegend), CD16(3G8, Biolegend), CD66b(G10F5, eBioscience), CD3(OKT3, eBioScience). Samples were acquired on a BD LSRII and analyzed on FlowJo (v10).
Vacutainer glass collection tubes with heparin
Manufactured by BD
Vacutainer glass collection tubes with heparin are a type of medical laboratory equipment used for blood sample collection. The primary function of these tubes is to collect and store blood samples for various medical tests and analyses. The tubes contain heparin, an anticoagulant, which helps prevent the blood from clotting during the collection and storage process.
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Lab products found in correlation
3 protocols using vacutainer glass collection tubes with heparin
1
PBMC Isolation and Characterization
2
Comprehensive PBMC Isolation and Characterization
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Blood was recovered in Vacutainer glass collection tubes with heparin (BD Biosciences). PBMC were isolated by gradient centrifugation with Histopaque-1077. Plasma was recovered for cytokine quantification by cytokine bead array (BD Biosciences) and resistin ELISA (Peprotech). Cell aliquots were frozen in liquid nitrogen. Following blood draw, PBMC isolation was performed within 24 h through density gradient centrifugation, and cells were stored immediately in liquid nitrogen. Flow cytometry characterization of PBMC involved incubation with Human TruStain FcX™ (Biolegend) and staining with primary Abs: CD14 (HCD14, Biolegend), CD16 (3G8, Biolegend), CD66b (G10F5, eBioscience), CD3 (OKT3, eBioScience). Samples were acquired on a BD LSRII and analyzed on FlowJo (v10).
3
Cytokine and Resistin Quantification
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All peripheral venous blood samples were recovered in Vacutainer glass collection tubes with heparin (BD Biosciences), transported at room temperature and processed within 24 hours. Gradient centrifugation with Histopaque-1077 was performed and plasma was recovered for cytokine quantification by cytokine bead array (BD Biosciences), and resistin ELISA (Peprotech). All reagents and plastics used were endotoxin-free.
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