Kc junior programme

Manufactured by Agilent Technologies

Sourced in United States

The KC Junior programme is a laboratory equipment offering from Agilent Technologies. It provides core functionalities for laboratory operations without further interpretation or extrapolation.

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Lab products found in correlation

Elx800 microplate reader, by Agilent Technologies (1 mentions) Il 10, by Cloud-Clone (1 mentions) Tnf α, by Cloud-Clone (1 mentions) Interleukin 2 (il 2), by Cloud-Clone (1 mentions)

2 protocols using kc junior programme

Quantifying Bovine Immune Biomarkers

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The blood samples were examined using commercial ELISA kits according to the manufacturer’s instructions for the following parameters: a concentration of acute phase proteins (APPs) including serum amyloid A (SAA; Tridelta Development Ltd.), haptoglobin (Hp; Tridelta Development Ltd.), alpha-2-Macroglobulin (A2M; Cusabio) and albumin (Alb, BSA; Cloud-Clone Corp.) as well as cytokine activity such as IL-1, IL-2, IL-10 and TNF-α (Cloud-Clone Corp.).
The optical densities in the microwells for APP and cytokine concentrations as well as for the antibodies to M. bovis and M. bovis antigen detection were read by an automated plate reader (Elx800 Microplate Reader, BioTek Instruments, Inc., USA) using the KC Junior programme (BioTek Instruments, Inc., USA).

Dudek K., Bednarek D., Ayling R.D., Kycko A, & Reichert M. (2019). Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia. BMC Veterinary Research, 15, 371.

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Salivary Lactoferrin Quantification by ELISA

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An enzyme-linked immunosorbent assay (ELISA) method was used to determine the salivary lactoferrin (Lf) concentration using MaxiSorp^TM microplates (Nunc Brand cat. no 449824, Roskilde, Denmark) coated with rabbit anti human lactoferrin (primary) antibody 1:500 (Sigma, L3262, St Louis, MO) and blocked with 2% bovine serum albumin (BSA; Sigma, A7030) according to Waszkiewicz et al. [32 (link)]. The results were analyzed by the KC Junior programme (Bio-Tek software applications for MS-DOS), all manual and automated readers.

Kobus A., Kierklo A., Zalewska A., Kuźmiuk A., Szajda S.D., Ławicki S, & Bagińska J. (2017). Unstimulated salivary flow, pH, proteins and oral health in patients with Juvenile Idiopathic Arthritis. BMC Oral Health, 17, 94.

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