5 hydroxyectoine

Ectoine was a kind gift from bitop AG (Witten, Germany). 5-Hydroxyectoine and glycine betaine were purchased from Merck KGaA (Darmstadt, Germany). Homoectoine was synthesized according to previously reported procedures (Koichi et al., 1991 ; Schnoor et al., 2004 (link)). Dithiothreitol (DTT) was purchased from AppliChem (Darmstadt, Germany) and phenylmethylsulfonyl fluoride (PMSF) from Roche (Basel, Switzerland). Desthiobiotin, Strep-TactinSuperflow chromatography material for the purification of proteins fused to a Strep-tag II affinity peptide and anhydrotetracycline hydrochloride (AHT) for the induction of the transcriptional activity of the TetR-regulated tet promoter present on the ectD expression plasmids pMP40 (ectD from Sphingopyxis alaskensis) and pMP41 (ectD from Pseudomonas stutzeri) (Widderich et al., 2014a (link)) were purchased from IBA GmbH (Göttingen, Germany). All other chemicals were purchased from Karl Roth GmbH (Karlsruhe, Germany), Merck KGaA (Darmstadt, Germany), and Sigma-Aldrich (Steinheim, Germany).

The antibiotics gentamycin, rifampicin, and kanamycin were obtained from Serva (Heidelberg, Germany); ampicillin was purchased from Carl Roth GmbH (Karlsruhe, Germany). Anhydrotetracycline hydrochloride, desthiobiotin, and Strep-Tactin Superflow chromatography material were obtained from IBA GmbH (Göttingen, Germany). Marker proteins for size exclusion chromatography experiments were purchased from Sigma-Aldrich (Taufkirchen, Germany). Restriction endonucleases and DNA ligase were obtained from ThermoScientific (St. Leon-Rot, Germany) and used as suggested by the manufacturer. Ectoine was a kind gift from the bitop AG (Witten, Germany) and 5-hydroxyectoine was purchased from Merck (Darmstadt, Germany). γ-ADABA was purchased from abcr GmbH (Karlsruhe, Germany).